Abstract
Transformed roots ofCucumis sativus were obtained from cotyledon tissues that had been bombarded with gold particles coated with plasmid pE7.4 using a pneumatic particle gun. This plasmid containsrolA, rolB, rolC genes and ORF 13 of the 7.4 kbEco RI fragment of T-DNA of pRi 1724 isolated fromAgrobacterium rhizogenes MAF 03-01724. The nature of the tissue and the composition of the culture media used greatly influenced the recovery of transformed roots. The transgenic nature of the derived roots was confirmed by the vigorous. highly-branched growth seen on a phytohormone-free medium. The stable integration ofrol genes into the cucumber genome was confirmed by Southern blot analysis.
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Chilton, M.-D., Tepfer, D.A., Petit, A., David, C., Casse-Delbart, F. and Tempé, J. (1982)Agrobacterium rhizogenes inserts T-DNA into the genomes of the host plant root cells.Nature 295, 432–4.
De Cleene, M. and De Ley, J. (1981) The host range of infectious hairy-root.Bot. Rev. 47, 147–94.
Graves, A.E., Goldman, S.L., Banks, S.W. and Graves, A.C.F. (1988) Scanning electron microscope studies ofAgrobacterium tumefaciens attachment toZea mays, Gladiolus sp., andTriticum aestivum.J. Bacteriol. 170, 2395–400.
Handa, T. (1992) Regeneration and characterization of prairie gentian (Eustoma grandiflorum) plants transformed byAgrobacterium rhizogenes.Pl. Tissue Culture Lett. 9, 10–4.
Iida, A., Seki, M., Kamada, M., Yamada, Y. and Morikawa, H. (1990) Gene delivery into cultured plant cells by DNA-coated gold particles accelerated by a pneumatic particle gun.Theor. Appl. Genet. 80, 813–6.
Iida, A., Yamashita, T., Yamada, Y. and Morikawa, H. (1991) Efficiency of particle-bombardment-mediated transformation is influenced by cell cycle stage in synchronized cultured cells of tobacco.Pl. Physiol. 97 1585–7.
Morikawa, H., Iida, A. and Yamada, Y. (1989) Transient expression of foreign genes in plant cells and tissues obtained by a simple biolistic device (particle-gun).Appl. Microbiol. Biotechnol. 31, 320–2.
Murashige, T. and Skoog, F. (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture.Physiol. Plant. 15, 473–97.
Murray, M.G. and Thompson, W.F. (1980) Rapid isolation of high molecular weight DNA.Nucl. Acids Res. 8, 4321–5.
Seki, M., Komeda, Y., Iida, A., Yamada, Y. and Morikawa, H. (1991) Transient expression of β-glucuronidase inArabidopsis thaliana leaves and roots andBrassica napus stems using a pneumatic particle gun.Pl. Mol. Biol. 17, 259–63.
Spéna, A., Schmülling, T., Konez, C. and Schell, J.S. (1987) Independent and synergistic activity ofrolA, B andC loci in stimulating abnormal growth in plants.EMBO J. 6, 3891–9.
Tepfer, D. (1984) Transformation of several species of higher plants byAgrobacterium rhizogenes: sexual transmission of the transformed genotype and phenotype.Cell 37, 959–67.
Visser, R.G.F., Jacobsen, E., Witholt, B. and Feenstra, W.J. (1989) Efficient transformation of potato (Solanum tuberosum L.) using a binary vector inAgrobacterium rhizogenes.Theor. Appl. Genet. 78, 594–600.
White, P.R. (1963)The cultivation of animal and plant cells. New York, USA: Ronald Press.
White, F.F., Taylor, B.H., Huffman, G.A., Gordon, M.P. and Nester, E.W. (1985) Molecular and genetic analysis of the transferred DNA regions of the root-inducing plasmid ofAgrobacterium rhizogenes.J. Bacteriol. 164, 33–44.
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Kodama, H., Irifune, K., Kamada, H. et al. Transgenic roots produced by introducing Ri-rol genes into cucumber cotyledons by particle bombardment. Transgenic Research 2, 147–152 (1993). https://doi.org/10.1007/BF01972608
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DOI: https://doi.org/10.1007/BF01972608