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Transient expression of foreign genes in plant cells and tissues obtained by a simple biolistic device (particle-gun)

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Summary

Successful transient expression of genes (luciferase and β-glucuronidase) in cultured tobacco cells and adventitious shoots of egg-plant hypocotyls can be obtained by use of a simple biolistic device. This device is driven by controlled gas pressure from a cylinder of nitrogen gas. It has the advantages of being free of explosive heat and of avoiding cell damage caused by expanding gas, due to the “self-sealing” effect of the projectile.

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Authors and Affiliations

  1. Research Center for Cell and Tissue Culture, Faculty of Agriculture, Kyoto University, 606, Kyoto, Japan

    Hiromichi Morikawa, Asako Iida & Yasuyuki Yamada

Authors
  1. Hiromichi Morikawa
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  2. Asako Iida
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  3. Yasuyuki Yamada
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Morikawa, H., Iida, A. & Yamada, Y. Transient expression of foreign genes in plant cells and tissues obtained by a simple biolistic device (particle-gun). Appl Microbiol Biotechnol 31, 320–322 (1989). https://doi.org/10.1007/BF00258417

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  • DOI: https://doi.org/10.1007/BF00258417

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