Chloride movement across the basolateral membrane of proximal tubule cells

Summary

Electrophysiologic and tracer experiments have shown that Cl⁻ entersNecturus proximal tubule cells from the tubule lumen by a process coupled to the flow of Na⁺, and that Cl⁻ entry is electrically silent. The mechanism of Cl⁻ exit from the cell across the basolateral membrane has not been directly studied. To evaluate the importance of the movement of Cl⁻ ions across the basolateral membrane, the relative conductance of Cl⁻ to K⁺ was determined by a new method. Single-barrel ion-selective microelectrodes were used to measure intracellular Cl⁻ and K⁺ as a function of basolateral membrane PD as it varied normally from tubule to tubule. Basolateral membrane Cl⁻ conductance was about 10% of K⁺ conductance by this method. A second approach was to voltage clamp the basolateral PD to 20 mV above and below the spontaneous PD, while sensing intracellular Cl⁻ activity with the second barrel of a double-barrel microelectrode. An axial wire electrode in the tubule lumen was used to pass current across the tubular wall and thereby vary the basolateral membrane PD. Cell Cl⁻ activity was virtually unaffected by the PD changes. We conclude that Cl⁻ leavesNecturus proximal tubule cells by a neutral mechanism, possibly coupled to the efflux of Na⁺ or K⁺.