Summary
In order to study how immune-inflammatory responses are involved in the pathogenesis of cerebral vasospasm after subarachnoid haemorrhage (SAH), the kinetics of expression of the intercellular adhesion molecule 1 (ICAM-1), a ligand for the leucocyte adhesion receptor, were studied on the cerebral arteries following SAH in rats.
The SAH was induced by intracisternal injection of arterial blood. The rats were sacrificed at specified times: immediately after induction of SAH to seven days after SAH. Cryostat sections of the basilar artery (BA) were prepared and incubated with anti-rat ICAM-1 antibody. Morphometric analysis of the BA revealed a significant narrowing of the luminal diameter on Day 2 following SAH. While in the non-treated normal animals, no nor only weak expression of ICAM-1 was observed on the endothelial layer of the BA, there was greater expression of ICAM-1 on the endothelial layer of the BA in SAH rats, and the expression was observed also in the medial layer of the artery from Day 2 to Day 5 following SAH.
The present results indicate that SAH really causes responses in the cellular immunity not only in the endothelial layer, but also in the medial layer of the artery as a target of immune damage, which is presumed to be one of the important steps in the development of cerebral vasospasm.
Similar content being viewed by others
References
Adams DH, Hubscher SG, Shaw J, Rothlein R, Neuberger JM (1989) Intercellular adhesion molecule 1 on liver allografts during rejection. Lancet 11: 1122–1125
Altmann DM, Hogg N, Trowsdale J, Wilkinson D (1989) Cotransfection of ICAM-1 and HLA-DR reconstitutes human antigen-presenting cell function in mouse L cells. Nature 338: 512–514
Bierer BA, Burakoff SJ (1988) T-cell adhesion molecules. FASEB J 2: 2584–2590
Boyd AW, Wawryk SO, Burns GF, Fecondo JV (1988) Intercellular adhesion molecule 1 (ICAM-1) has a central role in cellcell contact-mediated immune mechanisms. Proc Natl Acad Sci USA 85: 3095–3099
Cosimi AB, Conti D, Delmonico FL, Preffer FI, Wee SL, Rothlein R, Faanes R, Colvin RB (1990) In vivo effects of monoclonal antibody to ICAM-1 (CD 54) in nonhuman primates with renal allografts. J Immunol 144: 4604–4612
Crompton MR (1964) The pathogenesis of cerebral infarction following the rupture of cerebral artery aneurysm. Brain 87: 491–510
Delgado TJ, Brismar J, Svendgaard NAa (1985) Subarachnoid hemorrhage in the rat: angiography and fluorescence microscopy of the major crebral arteries. Stroke 16: 595–602
Dustin ML, Rothlein R, Bhan AK, Dinarello CA, Springer TA (1986) Induction by IL-1 and interferon-r, tissue distribution, biochemistry, and function of a natural adherence molecule (ICAM-1). J Immunol 137: 245–254
Handa Y, Hayashi M, Takeuchi H, Kobayashi H, Kawano H, Kabuto M (1991) Effect of cyclosporine on the development of cerebral vasospasm in a primate model. Neurosurgery 28: 380–386
Handa Y, Kabuto M, Kobayashi H, Kawano H, Takeuchi H, Hayashi M (1991) The correlation between immunological reaction in the arterial wall and the time course of the development of cerebral vasospasm in a primate model. Neurosurgery 28: 542–549
Harlan JM (1985) Leukocyte-endothelial interactions. Blood 65: 513–523
Hoshi T, Shimizu T, Kito K, Yamasaki N, Takahashi K, Takahashi M, Okada T, Kasuya H, Kitamura K (1984) Immunological study of late cerebral vasospasm in subarachnoid hemorrhage: detection of immunoglobulins, C 3 and fibrinogen in cerebral arterial walls by immunofluorescence methods. Neurol Med Chir 24: 647–654
Hughes JT, Schianchi PM (1978) Cerebral arterial spasm. A histological study at necropsy of the blood vessels in cases of subarachnoid hemorrhage. J Neurosurg 48: 515–525
Iigo Y, Takashi T, Tamatam T, Miyasaka M, Higashida T, Yagita H, Okumura K, Tsukuda W (1991) ICAM-1-dependent pathways is critically involved in the pathogenesis of adjuvant arthritis in rats. J Immunol 147: 4167–4171
Inaba K, Steinman RM (1984) Resting and sensitized lymphocytes exhibit distinct stimulatory (antigen-presenting cell) requirements for growth and lymphokine release. J Exp Med 160: 1717–1735
Kubota Te, Handa Y, Tsuchida A, Kaneko A, Kobayashi H, Kubota T (1993) The kinetics of lymphocytes subsets and macrophage in subarachnoid space after subarachnoid hemorrhage in rats. Stroke 24: 1993–2001
Maio M, Tessitori G, Pinto A, Temponi M, Colombatti A, Ferone S (1989) Differential role of distinct determinants of intercellular adhesion molecule-1 in immunologic phenomena. J Immunol 143: 181–188
Østergaard JR, Kreistensen BO, Svehag SE, Teisner B, Miletic T (1987) Immune complexes and complement activation following rupture of intracranial saccular aneurysm. J Neurosurg 66: 891–897
Peerless SJ, Kassell NF, Komatsu K, Hunter IG (1980) Cerebral vasospasm. Acute proliferative vasculopahty? II. morphology. In: Wilkins RH (ed) Cerebral arterial spasm. Williams and Wilkins, Baltimore, pp 88–96
Pellettieri L, Carlsson C-A, Lindholm L (1981) Is the vasospasm following subarachnoid hemorrhage an immunoreactive disease? Experientia 37: 1170–1171
Peterson JW, Nishizawa S, Hackett JD, Bun T, Teramura A, Zervas NT (1990) Cyclosporine A reduces cerebral vasospasm after subarachnoid hemorrhage in dogs. Stroke 21: 133–137
Poston RN, Haskard DO, Coucher JR, Gall NP, Jhonson-Tidey RR (1992) Expression of intercellular adhesion molecule-1 in atherosclerotic plaques. Am J Pathol 140: 665–672
Rothlein R, Czajkowski M, O'Neill MM, Marlin SD, Mainolfi E, Merluzzi VJ (1988) Induction of ICAM on primary and continuous cell lines by proinfiammatory cytokines. J Immunol 141: 1665–1669
Ryba M, Iwanska K, Walski M, Pastuzko M (1991) Immunomodulators interfere with angiopathy but not vasospasm after subarachnoid hemorrhage in rabbits. Acta Neurochir (Wien) 108: 81–84
Ryba M, Pastuszko M, Bidzínski J, Iwanska K, Dziewiecki C (1991) Cyclosporine A prevents neurological deterioration of patients with SAH — a preliminary report. Acta Neurochir (Wien) 112: 25–27
Sasaki T, Kassell NF, Yamashita M, Fujiwara S, Zuccarello M (1985) Barrier disruption in the major cerebral arteries following experimental subarachnoid hemorrhage. J Neurosurg 63: 433–440
Smith CW, Rothlein R, Hughes BJ, Mariscalo MM, Salmoksteig KC, Anderson D (1988) Recognition of an endothelial determinant for CD-18 dependent human neutrophil adherence and transendothelial migration. J Clin Invest 82: 1746–1756
Vejlsgaard CL, Ralfkiaer E, Avnstorp C, Czajkowski M, Marlin SD, Rothlein R (1989) Kinetics and characterization of intercellular adhesion molecule-1 (ICAM-1) expression on keratinocytes in various inflammatory skin lesions and malignant cutaneous lymphoma. J Am Acad Dermatol 20: 782–790
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Handa, Y., Kubota, T., Kaneko, M. et al. Expression of intercellular adhesion molecule 1 (ICAM-1) on the cerebral artery following subarachnoid haemorrhage in rats. Acta neurochir 132, 92–97 (1995). https://doi.org/10.1007/BF01404854
Issue Date:
DOI: https://doi.org/10.1007/BF01404854