Summary
Quantities of disencrusted sub-elementary cellulose fibrils from the cell wall of rose cells culturedin vitro were prepared. Following an X-ray and electron diffraction analysis, these fibrils gave a cellulose diffraction pattern which presented only two strong equatorial diffraction spacings at 0.409 and 0.572 nm indicating that the fibrils have a crystalline structure resembling that of cellulose IVI. This observation is best explained in terms of a lateral disorganization of the cellulose chains within the fibrils. This disorganization cannot be eliminated and is connected with the small width of the fibrils which contain from 12 to 25 cellulose chains only. In these fibrils, most of the cellulose chains are superficial and not locked with neighboring chains in a tight hydrogen bond system as in thicker cellulose microfibrils.
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Bjorndal, H., Lindberg, B., Svensson, S., 1967: Mass spectrometry of partially methylated acetates. Carbohydr. Res.5, 433.
—,Hellerqvist, C. G., Lindberg, B., Svensson, S., 1970: Gas liquid chromatography and mass spectrometry in methylation analysis of polysaccharides. Angew. Chem., Intern. edit.9, 610.
Blackwell, J., Kolpak, F. J., 1975: The cellulose microfibril as an imperfect array of elementary fibrils. Macromolecules8, 322.
Caulfield, D. F., 1971: Crystallite sizes in wet and dryValonia ventricosa. Textile Res. J.41, 267.
Chanzy, H., Imada, K., Vuong, R., 1978: Electron diffraction from the primary wall of cotton fibers. Protoplasma94, 299.
Colvin, J. R., 1972: The structure and biosynthesis of cellulose. CRC critical Reviews in Macromolecular Science1, 47.
Franke, W. W., Ermen, B., 1969: Negative staining of plant slime cellulose: an examination of the elementary fibril concept. Z. Naturforsch.24 b, 918.
Frey-Wyssling, A., Mühlethaler, K., 1951: The fine structure of cellulose. Fortschritte der Chemie organischer Naturstoffe8, 1. Wien: Springer.
Frey-Wyssling, A., 1969: The ultrastructure and biogenesis of native cellulose. Fortschritte der Chemie organischer Naturstoffe27, 1. Wien-New York: Springer.
Hanna, R. B., Côté, W. A., Jr., 1974: The sub-elementary fibril of plant cell wall cellulose. Cytobiol.10, 102.
Herth, W., Meyer, Y., 1977: Ultrastructural and chemical analysis of the wall fibrils synthesized by tobacco mesophyll protoplasts. Biol. Cellulaire30, 33.
Hess, K., Wergin, W., Kiessig, H., Engel, W., Philippoff, W., 1939 a: Untersuchungen über die Ontogenese und den chemischen Aufbau der pflanzlichen Zellwand. Naturwiss.37, 622.
—,Kiessig, H., Wergin, W., Engel, W., 1939 b: Zur Kenntnis der Bildung von Cellulose in der Zellwand. Ber. dtsch. chem. Ges.72 B, 642.
Heyn, A. N., 1969: The elementary fibril and supermolecular structure of cellulose in softwood fiber. J. Ultrastruc. Res.26, 52.
Howson, J. A., Sisson, W. A., 1954: Structure and properties of cellulose fibers. Submicroscopic structure. I. Cellulose and cellulose derivatives (Ott, E., Spurlin, H. M., Grafflin, M. W., eds.), Pt I, p. 231. New York: Wiley Interscience Publishers.
Hustache, G., Mollard, A., Barnoud, F., 1975: Culture illimitée d'une souche anergiée deRosa glauca par la technique des suspensions cellulaires, C. R. Acad. Sci. (Paris)281, 1381.
Imada, K.,Chanzy, H., 1976: Crystallographic features of cellulose in primary cell wall of higher plants. Eighth International Symposium on Carbohydrate Chemistry, Kyoto.
Kuniak, L., 1969: The polymolecularity of microcrystalline cellulose. Cellulose Chem. and Technol.3, 555.
Mackie, W., Preston, R. D., 1968: The occurrence of Mannan microfibrils in the green AlgaeCodium fragile andAcetabularia crenulata. Planta79, 249.
Marx-Figini, M., 1969: On the biosynthesis of cellulose in higher and lower plants. J. Polym. Sci., part C28, 57.
Mollard, A., Vuong, R., Chanzy, H., Barnoud, F., 1973 a: Ultrastructure de la cellulose dans les tissus de rosier cultivésin vitro. Physiol. Vég.11, 407.
—,Hustache, G., Barnoud, F., 1973 b: Les polysaccharides de la paroi cellulaire dans les tissus de Rosier cultivésin vitro. Importance des formes polymères du galactose dans quatre souches deRosa glauca. Comparaison avec le tissu cambial initial. Physiol. Vég.11, 539.
—,Barnoud, F., Dutton, G. G. S., 1976 a: Les galactanes des tissus deRosa glauca cultivésin vitro. Physiol. Vég.14, 101.
— —, 1976 b: Une glucane hémicellulosique β1 → 3 dans les parois des cellules de Rosier cultivéesin vitro. Physiol. Vég.14, 233.
— —, 1976 c: Une xyloglucane hémicellulosique β1 → 6 dans les cellules deRosa New Dawn cultivéesin vitro. Physiol. Vég.14, 241.
Morehead, F. F., 1950: Ultrasonic disintegration of cellulose fibers before and after acid hydrolysis. Textile Res. J.20, 549.
Mühlethaler, K., 1960: Die Feinstruktur der Zellulosemikrofibrillen. Z. Schweiz. Forstr.30, 55.
Nieduszynski, I., Preston, R. D., 1970: Crystallite size in natural cellulose. Nature225, 273.
Nobécourt, P., 1946: Culture prolongée de tissus végétaux en l'absence de facteurs de croissance. C. R. Acad. Sci. (Paris)222, 817.
Nowak-Ossorio, M., Gruber, E., Schurz, J., 1976: Untersuchungen zur Cellulose-Bildung in Baumwollsamen. Protoplasma88, 255.
Ohad, I., Danon, D., 1964: On the dimensions of cellulose microfibrils. J. Cell Biol.22, 302.
Preston, R. D., 1971: Negative staining and cellulose microfibril size. J. Microsc.93, part I, 7.
Roland, J. C., Vian, B., Reis, D., 1975: Observation with cytochemistry and ultracryotomy on the fine structure of the expanding walls in actively elongating plant cells. J. Cell Sci.19, 239.
Ruel, K., Comtat, J., Barnoud, F., 1977: Localisation histologique et ultrastructurale des xylanes dans les parois primaires des tissus d'Arundo donax. C. R. Acad. Sci. D284, 1421.
Sandford, P. A., Conrad, H. E., 1966: The structure of theAerobacter aerogenes A 3 (S1) polysaccharide. I. A reexamination using improved procedures for methylation analysis. Biochem.5, 1508.
Sawardeker, J. S., Sloneker, J. H., Jeanes, A., 1965: Quantitative determination of monosaccharides and their acetates by gas liquid chromatography. Anal. Chem.37, 1602.
Seaman, J. F., Moore, W. E., Mitchell, L., Millet, M. A., 1954: Techniques for the determination of pulp constituents by quantitative paper chromatography. TAPPI37, 336.
— —,Millet, M. A., 1963: Sugar units present, hydrolysis and quantitative paper chromatography. In: Methods in Carbohydrate Chemistry, Vol. 3, p. 54 (Whistler, R. L., ed.). New York-London: Academic Press.
Sisson, W. A., 1937: Identification of crystalline cellulose in young cotton fibers by X-ray diffraction analysis. Contrib. Boyce Thompson Inst.8, 389.
Sprague, B. S., Riley, J. L., Noether, H. D., 1958: Factors influencing the crystal structure of cellulose triacetate. Textile Res. J.28, 275.
StJohn Manley, R., 1964: Fine structure of native cellulose microfibrils. Nature204, 1155.
Sueoka, A.,Hayashi, J.,Watanabe, S., 1973: Differences between native cellulose I and regenerated cellulose I (I′). Nippon Kagaku Kaishi, 594.
Talmadge, K. W., Bauer, W. D., Keegstra, K., Albersheim, P., 1973: The macromolecular components of the walls of suspension cultured Sycamore cells (the pectic polysaccharides). Plant Physiol.51, 158.
Wardrop, A. B., 1949: Micellar organisation in primary cell walls. Nature164, 366.
Warwicker, J. O., 1967: Effect of chemical reagents on the fine structure of cellulose. Part IV: Action of caustic soda on the fine structure of cotton and ramie. J. Polymer Sci.A 1, 5, 2579.
Wellard, H. J., 1954: Variations in the lattice spacing of cellulose. J. Polymer Sci.13, 471.
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Chanzy, H., Imada, K., Mollard, A. et al. Crystallographic aspects of sub-elementary cellulose fibrils occurring in the wall of rose cells culturedin vitro . Protoplasma 100, 303–316 (1979). https://doi.org/10.1007/BF01279318
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DOI: https://doi.org/10.1007/BF01279318