Abstract
Experiments on short-term photosynthesis in H14CO3 - (2–5 s) using various species of different algal classes resulted in predominant 14C-labelling (>90% of total 14C-incorporation) of phosphorylated compounds. The percentage of malate and aspartate usually accounts for distinctly less than 10% of the total 14C-labelling. These findings are consistent with data from enzymatic analyses, since 97–100% of the carboxylation capacity is due to ribulose-1.5-biphosphate carboxylase (EC 4.1.1.39) in Rhodophyceae and Chlorophyceae. Phaeophyceae are generally characterized by considerable activity of phosphoenolpyruvate carboxykinase (EC 4.1.1.32): at least 10% of carboxylation is confined to this enzyme. Similar ratios are obtained when rates of photosynthesis and of light-independent CO2-fixation are compared. Activity of phosphoenolpyruvate carboxylase (EC 4.1.1.31) could not be detected in the species investigated. The results are discussed with emphasis on the pathway of photosynthetic carbon assimilation in marine algae.
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Abbreviations
- PEP-CK:
-
phosphoenolpyruvate carboxykinase (EC 4.1.1.32)
- PEP-C:
-
phosphoenolpyruvate carboxylase (EC 4.1.1.31)
- RubP-C:
-
ribulose-1.5-biphosphate carboxylase (EC 4.1.1.39)
References
Akagawa, H., Ikawa, T., Nisizawa, K.: Initial pathway of dark 14CO2-fixation in brown algae. Bot. Marina 15, 119–125 (1972a)
Akagawa, H., Ikawa, T., Nisizawa, K.: 14CO2-fixation in marine algae with special reference to the dark fixation in brown algae. Bot. Marina 15, 126–132 (1972b)
Akagawa, H., Ikawa, T., Nisizawa, K.: The enzyme system for the entrance of 14CO2 in the dark fixation of brown algae. Plant Cell Physiol. 13, 999–1016 (1972c)
Andrews, T.J., Hatch, M.D.: Activity and properties of ribulosediphosphate carboxylase from plants with the C4-dicarboxylic acid pathway of photosynthesis. Phytochemistry 10, 9–15 (1971)
Bassham, J.A.: Kinetic studies of the photosynthetic carbon reduction cycle. Ann. Rev. Plant Physiol. 15, 101–120 (1964)
Black, C.C.: Photosynthetic carbon fixation in relation to net CO2-uptake. Ann. Rev. Plant Physiol. 24, 253–286 (1973)
Craigie, J.S.: Dark fixation of 14C-bicarbonate by marine algae. Canad. J. Bot. 41, 317–325 (1963)
Hatch, M.D., Osmond, C.B., Slatver, R.O. (Eds.): Photosynthesis and Photorespiration. 565 pp. New York: Wiley Interscience 1971
Joshi, G.V., Karekar, M.D.: Pathway of 14CO2-fixation in marine algae. Proc. Ind. Natl. Sci. Acad. B 39, 489–493 (1973)
Joshi, G.V., Karekar, M.D., Gowda, C.A., Bhosale, L.: Photosynthetic carbon metabolism and carboxylating enzymes in algae and mangrove under saline conditions. Photosynthetica 8, 51–52 (1974)
Karekar, M.D., Joshi, G.V.: Photosynthetic carbon metabolism in marine algae. Bot. Marina 16, 216–220 (1973)
Kremer, B.P., Willenbrink, J.: CO2-Fixierung und Stofftransport in benthischen marinen Algen. I. Zur Kinetik der 14CO2-Assimilation bei Laminaria saccharina. Planta 103, 55–64 (1972)
Lobban, C.S.: A simple, rapid method of solubilizing algal tissue for scintillation counting. Limnol. Oceanogr. 19, 356–359 (1974)
Morris, I., Farrell, K.: Photosynthetic rates, gross patterns of carbon dioxide fixation and activities of ribulose diphosphate carboxylase in marine algae grown at different temperatures. Physiol. Plant. 25, 372–377 (1971)
Patil, B.A., Joshi, G.V.: Photosynthetic studies in Ulva lactuca. Ethanol soluble fraction. Bot. Marina 14, 22–23 (1971)
Raven, J.A.: Carbon dioxide fixation. In: Algal Physiology and Biochemistry, pp. 434–455, Stewart, W.D.P. (Ed.). Oxford: Blackwell 1974
Tyskiewicz, E.: An improved solvent system for the paper chromatography of phosphate esters. Anal. Biochem. 3, 164–172 (1962)
Weidner, M., Küppers, U.: Phosphoenolpyruvat-Carboxykinase und Ribulose-1.5-diphosphat-Carboxylase von Laminaria hyperborea (Gunn.) Fosl.: Das Verteilungsmuster der Enzymaktivitäten im Thallus. Planta 114, 365–372 (1973)
Willenbrink, J., Rangoni-Kübbeler, M., Tersky, B.: Frond development and CO2-fixation in Laminaria hyperborea. Planta 125, 161–170 (1975)
Yamaguchi, T., Ikawa, T., Nisizawa, K.: Pathway of mannitol formation during photosynthesis in brown algae. Plant Cell Physiol. 10, 425–440 (1969)
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Dedicated to Professor H. Fischer, Bonn, on his 65th birthday
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Kremer, B.P., Küppers, U. Carboxylating enzymes and pathway of photosynthetic carbon assmilation in different marine algae—Evidence for the C4-pathway?. Planta 133, 191–196 (1977). https://doi.org/10.1007/BF00391918
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DOI: https://doi.org/10.1007/BF00391918