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Crustacean cardioactive peptide in the nervous system of the locust, Locusta migratoria: an immunocytochemical study on the ventral nerve cord and peripheral innervation

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Summary

Crustacean cardioactive peptide-immunoreactive neurons occur in the entire central nervous system of Locusta migratoria. The present paper focuses on mapping studies in the ventral nerve cord and on peripheral projection sites. Two types of contralaterally projecting neurons occur in all neuromers from the subesophageal to the seventh abdominal ganglia. One type forms terminals at the surface of the thoracic nerves 6 and 1, the distal perisympathetic organs, the lateral heart nerves, and on ventral and dorsal diaphragm muscles. Two large neurons in the anterior part and several neurons of a different type in the posterior part of the terminal ganglion project into the last tergal nerves. In the abdominal neuromers 1–7, two types of ipsilaterally projecting neurons occur, one of which gives rise to neurosecretory terminals in the distal perisympathetic organs, in peripheral areas of the transverse, stigmata and lateral heart nerves. Four subesophageal neurons have putative terminals in the neurilemma of the nervus corporis allati II, and in the corpora allata and cardiaca. In addition, several immunoreactive putative interneurons and other neurons were mapped in the ventral nerve cord. A new in situ whole-mount technique was essential for elucidation of the peripheral pathways and targets of the identified neurons, which suggest a role of the peptide in the control of heartbeat, abdominal ventilatory and visceral muscle activity.

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Abbreviations

AG :

abdominal ganglia

AM :

alary muscle

AMN :

alary muscle nerve

CA :

corpus allatum

CC :

corpus cardiacum

dPSO :

distal perisympathetic organ

LHN :

lateral heart nerve

LT :

CCAP-immunoreactive lateral tract

NCA :

nervus corporis allati

NCC :

nervus corporis cardiaci

NM :

neuromer

PMN :

paramedian nerve

PSO :

perisympathetic organ

SOG :

subesophageal ganglion

VDM :

ventral diaphragm muscles

VNC :

ventral nerve cord

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Authors and Affiliations

  1. Institut für Zoophysiologie, Rheinische Friedrich-Wilhelms-Universität, Endenicher Allee 11-13, W-5300, Bonn 1, Germany

    Heinrich Dircksen & Rainer Keller

  2. Abteilung Zellbiologie, Max Planck Institut für Entwicklungsbiologie, W-7900, Tübingen, Germany

    Arno Müller

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  1. Heinrich Dircksen
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  2. Arno Müller
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  3. Rainer Keller
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Dircksen, H., Müller, A. & Keller, R. Crustacean cardioactive peptide in the nervous system of the locust, Locusta migratoria: an immunocytochemical study on the ventral nerve cord and peripheral innervation. Cell Tissue Res 263, 439–457 (1991). https://doi.org/10.1007/BF00327278

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