Skip to main content
Log in

Modulation of somatic embryogenesis in early and late-stage embryos of wheat (Triticum aestivum L.) under the influence of (±)-abscisic acid and its analogs

  • Published:
Plant Cell, Tissue and Organ Culture Aims and scope Submit manuscript

Abstract

Zygotic embryos from ten spring wheat (Triticum aestivum L.) genotypes were tested for embryogenic callus induction in the presence or absence of externally supplied (±)-abscisic acid (ABA) and two of its analogs, methyl abscisate and methyl epoxy-beta-ionylideneacetate. (±)-ABA and its analogs suppressed precocious germination of cultured late-stage embryos and promoted embryogenic callus induction. A significantly greater number of plants was regenerated from calli induced in the presence of ABA and ABA analogs. Early-stage embryos when cultured in the presence of (±)-ABA showed a negative response. Possible roles of ABA with respect to the expression of somatic embryogenesis are discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. Ahloowalia BS (1982) Plant regeneration from callus culture in wheat. Crop Sci 22: 405–410

    Google Scholar 

  2. Higgins P, Mathias RJ (1987) The effect of the 4B chromosomes of hexaploid wheat on the growth and regeneration of callus cultures. Theor Appl Genet 74: 439–444

    Article  Google Scholar 

  3. Lazar MD, Chen THH, Scoles GJ, Kartha KK (1987) Immature embryo and anther culture of chromosome addition lines of rye in chinese spring wheat. Plant Sci 51: 77–81

    Article  Google Scholar 

  4. Maddock SE, Lancaster VA, Risiott R, Franklin J (1983) Plant regeneration from cultured immature embryos and inflorescences of 25 cultivars of wheat (Triticum aestivum). J Exp Bot 34: 915–926

    Google Scholar 

  5. Mathias RJ, Fukui K (1986) The effect of specific chromosome and cytoplasm substitutions on the tissue culture response of wheat (Triticum aestivum) callus. Theor Appl Genet 71: 797–800

    Article  Google Scholar 

  6. Mathias RJ, Fukui K, Law CN (1986) Cytoplasmic effects on the tissue culture response of wheat (Triticum aestivum) callus. Theor Appl Genet 72: 70–75

    Article  Google Scholar 

  7. Milborrow BV (1974) The chemistry and physiology of abscisic acid. Ann Rev Plant Physiol 25: 259–307

    Article  Google Scholar 

  8. Milborrow BV, Robinson DR (1973) Factors affecting the biosynthesis of abscisic acid. J Exp Bot 24: 537–548

    Google Scholar 

  9. Morris PC, Weiler EW, Maddock SE, Jones MGK, Lenton JR, Bowles DJ (1988) Determination of endogenous abscisic acid levels in immature cereal embryos during in vitro culture. Planta 173: 110–116

    Google Scholar 

  10. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473–479

    Google Scholar 

  11. Oritani T, Yamashita K (1983) Synthesis and plant growth inhibitory activities of (±)-and (−)-(2Z,4E)-5-(1′,2′-epoxy-2′,6′, 6′-trimethylcyclohexyl)-3-methyl-2,4-pentadienoic acid. Phytochemistry 22: 1909–1912

    Article  Google Scholar 

  12. Quatrano RS (1987) The role of hormones during seed development. In: Davies PJ (Ed) Plant Hormones and their Role in Plant Growth and Development (494–514) Martinus Nijhoff Publishers, Lancaster

    Google Scholar 

  13. Quatrano RS, Ballo BL, Williamson JD, Hamblin MT, Mansfield M (1983) ABA-controlled expression of embryo-specific genes during wheat grain development. In: Goldberg R (Ed) Plant Molecular Biology (pp 343–353) Liss, New York

    Google Scholar 

  14. Rajasekaran K, Hein MB, Vasil IK (1987) Endogenous abscisic acid and indole-3-acetic acid and somatic embryogenesis in cultured leaf explants of Pennisetum purpureum Schum. J Plant Physiol 84: 47–51

    Google Scholar 

  15. Rajasekaran K, Hein MB, Davis GC, Carnes MG, Vasil IK (1987) Endogenous growth regulators in leaves and tissue cultures of Pennisetum purpureum Schum. J Plant Physiol 130: 13–25

    Google Scholar 

  16. Reddy VD, Reddy GM (1987) Effect of abscisic acid and gibberellic acid on morphogenesis in callus cultures of hexaploid triticale. J Plant Physiol 128: 303–306

    Google Scholar 

  17. Sears RG, Deckard EL (1982) Tissue culture variability in wheat: callus induction and plant regeneration. Crop Sci 22: 546–550

    Google Scholar 

  18. Shimida T (1978) Plant regeneration from callus induced from wheat embryos. Jap J Genet 53: 371–374

    Google Scholar 

  19. Tamura S, Nagao M (1970) Synthesis and biological activities on new plant growth inhibitors structurally related to abscisic acid. Agric Biol Chem 34: 1393–1401

    Google Scholar 

  20. Triplett BA, Quatrano RS (1982) Timing, localization, and control of wheat germ agglutinin synthesis in developing wheat embryos. Devel Biol 91: 491–496

    Article  Google Scholar 

  21. Vasil V, Vasil IK (1981) Somatic embryogenesis and plant regeneration from suspension cultures of pearl millet (Pennisetum americanum). Ann Bot 47: 669–678

    Google Scholar 

  22. Walton DC (1983) Structure-activity relationships of abscisic acid analogs and metabolites. In: Addicott FT (Ed) Abscisic Acid (pp 113–146) Praeger, New York

    Google Scholar 

  23. Walton DC, Sondheimer E (1972) Activity and metabolism of 14C-(±)-abscisic acid derivatives. Plant Physiol 49: 290–292

    Google Scholar 

  24. William RMJr, Bayley CC, Quatrano RS (1988) Regulation of a wheat promoter by abscisic acid in rice protoplasts. Nature (London) 335: 454–457

    Google Scholar 

  25. Williamson JD, Quatrano RS (1988) ABA regulation of two classes of embryo-specific sequences in mature wheat embryos. Plant Physiol 86: 208–215

    Google Scholar 

  26. Williamson JD, Quatrano RS, Cuming AC (1985) Em polypeptide and its messenger RNA levels are modulated by abscisic acid during embryogenesis in wheat. Eur J Biochem 152: 501–507

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Additional information

Dedicated to Dr. Friedrich Constabel on the occasion of his 60th birthday

Rights and permissions

Reprints and permissions

About this article

Cite this article

Qureshi, J.A., Kartha, K.K., Abrams, S.R. et al. Modulation of somatic embryogenesis in early and late-stage embryos of wheat (Triticum aestivum L.) under the influence of (±)-abscisic acid and its analogs. Plant Cell Tiss Organ Cult 18, 55–69 (1989). https://doi.org/10.1007/BF00033465

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00033465

Key words

Navigation