Abstract
Comparative measurements of nuclear DNA (Feulgen) after hydrolysis with 1 n HC1 at 60°C and 5 n HC1 at 20°C revealed that only the latter gives accurate results. Therefore, the problem of heterochromatin underreplication in Drosophila polytene chromosomes should be re-investigated using coldhydrolysed material, because older data may be the result of a non-suitable method and, therefore, erroneous.
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References
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Dennhöfer, L. (1979). Cytophotometric Measurements of DNA in Salivary Gland Nuclei of Drosophila melanogaster . In: Nagl, W., Hemleben, V., Ehrendorfer, F. (eds) Genome and Chromatin: Organization, Evolution, Function. Plant Systematics and Evolution, vol 2. Springer, Vienna. https://doi.org/10.1007/978-3-7091-8556-8_6
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DOI: https://doi.org/10.1007/978-3-7091-8556-8_6
Publisher Name: Springer, Vienna
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