Skip to main content
Log in

Differential replication of satellite DNA in polyploid tissues of Drosophila virilis

  • Published:
Chromosoma Aims and scope Submit manuscript

Abstract

Satellite DNA amounts were examined in adult tissues of Drosophila virilis, a species whose DNA contains three prominent satellites. Satellite amounts in DNA from six of the seven tissues were lower than in DNA from diploid (adult brain) tissue. Satellite amounts in adult ovary DNA, however, were equivalent to or greater than diploid levels. When DNA from pupal ovaries was examined, a 30% increase in satellite amounts over diploid levels was found. An RNA-DNA hybridization experiment showed that the ribosomal RNA genes in pupal ovary DNA were under-replicated relative to diploid DNA levels.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Ashburner, M.: Function and structure of polytene chromosomes during insect development. Advanc Insect Physiol. 7, 1–96 (1970)

    Google Scholar 

  • Atherton, D., Gall, J.: Salivary gland squashes for in situ nucleic acid hybridization studies. Dros. Inform. Service 49, 131–133 (1972)

    Google Scholar 

  • Beermann, W.: Riesenchromosomen. Protoplasmatologia VI D. Wien: Springer 1962

    Google Scholar 

  • Berendes, H., Keyl, H.: Distribution of DNA in heterochromatin and euchromatin of polytene nuclei of Drosophila hydei. Genetics 57, 1–13 (1967)

    Google Scholar 

  • Blumenfeld, M., Forrest, H.: Differential under-replication of satellite DNAs during Drosophila development. Nature (Lond.) New Biol. 239, 170–172 (1972)

    Google Scholar 

  • Bodenstein, D.: The post-embryonic development of Drosophila. In: Biology of Drosophila (M. Demerec, ed.), 275–367, 1950

  • Brady, T.: Feulgen cytophotometric determination of the DNA content of the embryo proper and suspensor cells of Phaseolus coccineus. Cell Differentiation 2, 65–75 (1973)

    Google Scholar 

  • Dawid, I. B.: Deoxyribonucleic acid in amphibian eggs. J. molec. Biol. 12, 581–599 (1965)

    Google Scholar 

  • Dickson, E., Boyd, J., Laird, C. D.: Sequence diversity of polytene chromosome DNA from Drosophila hydei. J. molec. Biol. 61, 615–627 (1971)

    Google Scholar 

  • Gall, J. G., Cohen, E. H., Polan, M. L.: Repetitive DNA sequences in Drosophila Chromosoma (Berl.) 33, 319–344 (1971)

    Google Scholar 

  • Gall, J.G., Macgregor, H. C., Kidston, M. E.: Gene amplification in the oocytes of Dytiscid water beetles. Chromosoma (Berl.) 26, 169–187 (1969)

    Google Scholar 

  • Gillespie, D., Spiegelman, S.: A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membrane. J. molec. Biol. 12, 829–842 (1965)

    Google Scholar 

  • Heitz, E.: Über α- und β-Heterochromatin sowie Konstanz und Bau der Chromomeren bei Drosophila. Biol. Zbl. 54, 588–609 (1934)

    Google Scholar 

  • Hennig, W., Meer, B.: Reduced polyteny of ribosomal RNA cistrons in giant chromosomes of Drosophila hydei. Nature (Lond.) New Biol. 233, 70–72 (1971)

    Google Scholar 

  • Ifft, J. B., Voet, D. H., Vinograd, J.: The determination of density distributions and density gradients in binary solutions at equilibrium in the ultracentrifuge. J. phys. Chem. 65, 1138–1145 (1961)

    Google Scholar 

  • Jacob, J., Sirlin, J. L.: Cell function in the ovary of Drosophila I. DNA classes in nurse cells as determined by autoradiography. Chromosoma (Berl.) 10, 210–228 (1959)

    Google Scholar 

  • Kavenoff, R., Zimm, B. H.: Chromosome-sized DNA molecules from Drosophila. Chromosoma (Berl.) 41, 1–27 (1973)

    Google Scholar 

  • King, R. C.: Ovarian development in Drosophila melanogaster. New York: Academic Press Inc. 1970

    Google Scholar 

  • King, R. C.: The cytology of the irradiated ovary of Drosophila melanogaster. Exp. Cell Res. 13, 545–552 (1957)

    Google Scholar 

  • King, R. C., Sang, J. H., Leth, C. B.: The hereditary ovarian tumors of the fes mutant of Drosophila melanogaster. Exp. Cell Res. 23, 108–117 (1961)

    Google Scholar 

  • King, R. C., Wolfsberg, M. F.: Oogenesis in adult D. melanogaster. VI. A comparison of oogenesis among D. melanogaster, virilis, pseudoobscura and gibberosa. Growth 21, 281–285 (1957)

    Google Scholar 

  • Kunz, W., Eckhardt, R. A.: Chromosomal distribution of satellite DNA in germ-line and somatic tissues of the gall midge, Heteropeza pygmaea. Chromosoma (Berl.) 47, 1–19 (1974)

    Google Scholar 

  • Makino, S.: A morphological study of the nucleus in various kinds of somatic cells of D. virilis. Cytologia (Tokyo) 9, 272–282 (1938)

    Google Scholar 

  • Mulder, M. P., Duijn, P. van, Gloor, H. J.: The replicative organization of DNA in polytene chromosomes of Drosophila hydei. Genetica ('s Gravenhage) 39, 385–428 (1968)

    Google Scholar 

  • Painter, T. S., Reindorp, E. C.: Endomitosis in the nurse cells of the ovary of D. melanogaster. Chromosoma (Berl.) 1, 276–283 (1939)

    Google Scholar 

  • Pearson, G. G., Timmis, J. N., Ingle, J.: The differential replication of DNA during plant development. Chromosoma (Berl.) 45, 281–294 (1974)

    Google Scholar 

  • Rasch, E.M.: DNA cytophotometry of salivary gland nuclei and other tissue systems in Dipteran larvae. In: Introduction to quantitative cytochemistry II (G. L. Wied and G. F. Bahr, eds.), p. 357–397 (1970)

  • Rasch, E. M., Barr, H. J., Rasch, R. W.: The DNA content of sperm of Drosophila melanogaster. Chromosoma (Berl.) 33, 1–18 (1971)

    Google Scholar 

  • Renkawitz-Pohl, R., Kunz, W.: Under-replication of satellite DNAs in polyploid ovarian tissue in Drosophila virilis. Chromosoma (Berl.), 49, 375–382 (1975)

    Google Scholar 

  • Ritossa, F., Spiegelman, S.: Localization of DNA complementary to ribosomal RNA in the nucleolus organizer region of Drosophila melanogaster. Proc. nat. Acad. Sci. (Wash.) 53, 737–745 (1965)

    Google Scholar 

  • Robb, J. A.: Maintenance of imaginal discs of Drosophila melanogaster in chemically defined media. J. Cell Biol. 41, 876–885 (1969)

    Google Scholar 

  • Rudkin, G. T.: Non-replicating DNA in Drosophila. Genetics 61, Suppl. 227–238 (1969)

    Google Scholar 

  • Rudkin, G. T.: Non-replicating DNA in giant chromosomes. Genetics 52, 470 (1965)

    Google Scholar 

  • Schweber, M. S.: The satellite bands of the DNA of Drosophila virilis. Chromosoma (Berl.) 44, 371–382 (1974)

    Google Scholar 

  • Sherman, M. I., McLaren, A., Walker, P. M. B.: Mechanism of accumulation of DNA in giant cells of mouse trophoblast. Nature (Lond.) New Biol. 238, 175–176 (1972)

    Google Scholar 

  • Spear, B. B., Gall, J. G.: Independent control of ribosomal gene replication in polytene chromosomes of D. melanogaster. Proc. nat. Acad. Sci. (Wash.) 70, 1359–1363 (1973)

    Google Scholar 

  • Swift, H.: Cytochemical techniques for nucleic acids. In: The nucleic acids, II (E. Chargaff and J. Davidson, eds.). New York: Academic Press, Inc. 1955

    Google Scholar 

  • Swift, H.: Nucleic acids and cell morphology in Dipteran salivary glands. In: The molecular control of cellular activity (J. Allen, ed.), p. 73–125 (1962)

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Endow, S.A., Gall, J.G. Differential replication of satellite DNA in polyploid tissues of Drosophila virilis . Chromosoma 50, 175–192 (1975). https://doi.org/10.1007/BF00283238

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00283238

Keywords

Navigation