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Molecular Genetics of Chloroplast Ribosomes In Chlamydomonas

  • Conference paper
The Translational Apparatus of Photosynthetic Organelles

Part of the book series: NATO ASI Series ((ASIH,volume 55))

Abstract

The green alga Chlamydomonas reinhardtii is an ideal model organism in which to investigate the cooperation of nuclear and chloroplast genomes in chloroplast ribosome formation from both developmental and evolutionary perspectives. Mutations affecting a variety of ribosomal components have been isolated and mapped genetically (see Boynton et al., 1990a and Harris, 1989 for reviews), and both the chloroplast (Boynton et al., 1988, 1990b; Blowers et al., 1989) and nuclear genomes (Debuchy et al., 1989; Kindle et al., 1989; Mayfield and Kindle, 1990; Diener et al., 1990) can be manipulated using newly developed transformation methods. In this paper we review our recent work on 1) the genetics and molecular biology of mutations to antibiotic resistance affecting the rRNA genes; 2) identification of chloroplast and nuclear genes encoding chloroplast ribosomal proteins (r-proteins); 3) expression of chloroplast genes encoding r-proteins; and 4) the transformation of chloroplast rRNA genes. We also outline the direction our current research is taking to define more precisely the rRNA-r-protein interactions involved in chloroplast ribosome biogenesis and function, as well as the molecular mechanisms involved in regulating expression of chloroplast r-protein genes.

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References

  • Andersson DI, Andersson SGE, Kurland CG (1986) Functional interactions between mutated forms of ribosomal proteins S4, S5 and S12. Biochimie 68:705–713

    Article  PubMed  CAS  Google Scholar 

  • Bartlett SG, Harris EH, Grabowy CT, Gillham NW, Boynton JE (1979) Ribosomal subunits affected by antibiotic resistance mutations at seven chloroplast loci in Chlamydomonas reinhardtii. Mol Gen Genet 176: 199–208

    CAS  Google Scholar 

  • Bennoun P, Delepelaire P, Delosme M (1981) A uniparental mutant of Chlamydomonas reinhardtii resistant to chloramphenicol. Curr Genet 3:251–253

    Article  CAS  Google Scholar 

  • Blowers AD, Bogorad L, Shark KB, Sanford JC (1989) Studies on Chlamydomonas transformation. Foreign DNA can be stably maintained in the chromosome. Plant Cell 1: 123–132

    Article  PubMed  CAS  Google Scholar 

  • Boynton JE, Gillham NW, Harris EH, Hosier JP, Johnson AM, Jones AR, Randolph Anderson BL, Robertson D, Klein TM, Shark KB, Sanford JC (1988) Chloroplast transformation in Chlamydomonas with high velocity microprojectiles. Science 240: 1534–1538

    Article  PubMed  CAS  Google Scholar 

  • Boynton JE, Gillham NW, Harris EH, Newman SM, Randolph-Anderson BL, Johnson AM, Jones AR (1990a) Manipulating the chloroplast genome of Chlamydomonas — molecular genetics and transformation. Current Research in Photosynthesis III: 509–516

    Google Scholar 

  • Boynton JE, Gillham NW, Newman SM, Harris EH (1990b) Organelle genetics and transformation in Chlamydomonas. In Hohn T (ed) Cell Organelles, Advances in Plant Gene Research, Vol 6. Springer-Verlag, in press

    Google Scholar 

  • Brimacombe R (1988) The emerging three-dimensional structure and function of 16S ribosomal RNA. Biochemistry 27: 4207–4214

    Article  PubMed  CAS  Google Scholar 

  • Bryant DA, Stirewalt VL (1990) The cyanelle genome of Cyanophora paradoxa encodes ribosomal proteins not encoded by the chloroplast genomes of higher plants. FEBS Lett 259: 273–280

    Article  PubMed  CAS  Google Scholar 

  • Choquet Y, Goldschmidt-Clermont M, Girard-Bascou J, Kiick U, Bennoun P, Rochaix J-D (1988) Mutant phenotypes support a trans-splicing mechanism for the expression of the tripartite psaA gene in the C. reinhardtii chloroplast. Cell 52:903–913

    Article  PubMed  CAS  Google Scholar 

  • Christopher DA, Hallick RB (1989) Euglena gracilis chloroplast ribosomal protein operon: a new chloroplast gene for ribosomal protein L5 and description of a novel organelle intron category designated group III. Nucl Acids Res 17: 7591–7608

    Article  PubMed  CAS  Google Scholar 

  • Christopher DA, Cushman JC, Price CA, Hallick RB (1988) Organization of ribosomal protein genes rpl23, rpl2, rpsl9, rpl22 and rps3 on the Euglena gracilis chloroplast genome. Curr Genet 14: 275–286

    Article  PubMed  CAS  Google Scholar 

  • Conde MF, Boynton JE, Gillham NW, Harris EH, Tingle CL, Wang WL (1975) Chloroplast genes in Chlamydomonas affecting organelle ribosomes. Genetic and biochemical analysis of antibiotic-resistant mutants at several gene loci. Mol Gen Genet 140: 183–220

    Article  PubMed  CAS  Google Scholar 

  • Cseplö A, Etzold T, Schell J, Schreier PH (1988) Point mutations in the 23S rRNA genes of four lincomycin resistant Nicotiana plwnbaginifolia mutants could provide new selectable markers for chloroplast transformation. Mol Gen Genet 214: 295–299

    Article  PubMed  Google Scholar 

  • Cundliffe E (1986) Involvement of specific portions of ribosomal RNA in defined ribosomal functions: a study using antibiotics. In Hardesty, B, Kramer G (eds) Structure, Function, and Genetics of Ribosomes. Springer-Verlag, pp. 586–604

    Chapter  Google Scholar 

  • Cundliffe E (1987) On the nature of antibiotic binding sites in ribosomes. Biochimie 69: 863–869

    Article  PubMed  CAS  Google Scholar 

  • Davidson JN, Hanson MR, Bogorad L (1974) An altered chloroplast ribosomal protein in ery-M1 mutants of Chlamydomonas reinhardi. Mol Gen Genet 132: 119–129

    PubMed  CAS  Google Scholar 

  • Davidson JN, Hanson MR, Bogorad L (1978) Erythromycin resistance and the chloroplast ribosome in Chlamydomonas reinhardi. Genetics 89: 281–297

    PubMed  CAS  Google Scholar 

  • Debuchy R, Purton S, Rochaix J-D (1989) The argininosuccinate lyase gene of Chlamydomonas reinhardtii: an important tool for nuclear transformation and for correlating the genetic and molecular maps of the ARG7 locus. EMBO J 8: 2803–2809

    PubMed  CAS  Google Scholar 

  • De Stasio EA, Dahlberg AE (1990) Effects of mutagenesis of a conserved base-paired site near the decoding region of Escherichia coli 16S ribosomal RNA. J Mol Biol 212:127-133

    Article  PubMed  Google Scholar 

  • Diener DR, Curry AM, Johnson KA, Williams BD, Lefebvre PA, Kindle KL, Rosenbaum JL (1990) Rescue of a paralyzed-flagella mutant of Chlamydomonas by transformation. Proc Nad Acad Sci USA 87: 5739–5743

    Article  CAS  Google Scholar 

  • Dron M, Rahire M, Rochaix J-D (1982a) Sequence of the chloroplast DNA region of Chlamydomonas reinhardii containing the gene of the large subunit of ribulose bisphosphate carboxylase and parts of its flanking genes. J Mol Biol 162:775–793

    Article  PubMed  CAS  Google Scholar 

  • Dron M, Rahire M, Rochaix J-D (1982b) Sequence of the chloroplast 16S rRNA gene and its surrounding regions of Chlamydomonas reinhardii. Nucl Acids Res 10:7609–7620

    Article  PubMed  CAS  Google Scholar 

  • Etzold T, Fritz CC, Schell J, Schreier PH (1987) A point mutation in the chloroplast 16S rRNA gene of a streptomycin resistant Nicotiana tabacum. FEBS Lett 219:343–346

    Google Scholar 

  • Fox TD, Costanzo MC, Strick CA, Marykwas DL, Seaver EC, Rosenthal JK (1988) Translational regulation of mitochondrial gene expression by nuclear genes of Saccharomyces cerevisiae. Phil Trans Royal Soc London B 319: 97–105

    Article  CAS  Google Scholar 

  • Fromm H, Edelman M, Aviv D, Galun E (1987) The molecular basis for rRNA-dependent spectinomycin resistance in Nicotiana chloroplasts. EMBO J 6: 3233–3237

    PubMed  CAS  Google Scholar 

  • Fromm H, Galun E, Edelman M (1989) A novel site for streptomycin resistance in the “530 loop” of chloroplast 16S ribosomal RNA. Plant Mol Biol 12: 499–505

    Article  CAS  Google Scholar 

  • Funatsu G, Wittmann HG (1972) Ribosomal proteins XXXIII. Location of amino acid replacements in protein S12 isolated from Escherichia coli mutants resistant to streptomycin. J Mol Biol 68: 547–550

    Article  PubMed  CAS  Google Scholar 

  • Galili S, Fromm H, Aviv D, Edelman M, Galun E (1989) Ribosomal protein S12 as a site for streptomycin resistance in Nicotiana chloroplasts. Mol Gen Genet 218:289–292

    Article  PubMed  CAS  Google Scholar 

  • Gauthier A, Turmel M, Lemieux C (1988) Mapping of chloroplast mutations conferring resistance to antibiotics in Chlamydomonas: Evidence for a novel site of streptomycin resistance in the small subunit rRNA. Mol Gen Genet 214:192–197

    Article  PubMed  CAS  Google Scholar 

  • Gutell RR, Weiser B, Woese CR, Noller HF (1985) Comparative anatomy of 16-S-like ribosomal RNA. Prog Nucl Acid Res Mol Biol 32: 155–216

    Article  CAS  Google Scholar 

  • Hallick RB (1984) Identification and partial DNA sequence of the gene for the a-subunit of the ATP synthase complex of Chlamydomonas reinhardii chloroplasts. FEBS Lett 177: 274–276

    Article  CAS  Google Scholar 

  • Harris EH (1989) The Chlamydomonas Sourcebook. Academic Press, San Diego, 780 pp.

    Google Scholar 

  • Harris EH, Boynton JE, Gillham NW, Tingle CL, Fox SB (1977) Mapping of chloroplast genes involved in chloroplast ribosome biogenesis in Chlamydomonas reinhardtii. Mol Gen Genet 155: 249–265

    Article  CAS  Google Scholar 

  • Harris EH, Burkhart BD, Gillham NW, Boynton JE (1989) Antibiotic resistance mutations in the chloroplast 16S and 23S rRNA genes of Chlamydomonas reinhardtii: Correlation of genetic and physical maps of the chloroplast genome. Genetics 123: 281–292

    PubMed  CAS  Google Scholar 

  • Harris EH, Boynton JE, Gillham NW, Burkhart BD, Newman SM (1990) Chloroplast genome organization in Chlamydomonas. Arch Protistenk, in press

    Google Scholar 

  • Hasenbank R, Guthrie C, Stöffler G, Wittmann HG, Rosen L, Apirion D (1973) Electrophoretic and immunological studies on ribosomal proteins of 100 Escherichia coli revenants from streptomycin dependence. Mol Gen Genet 127: 1–18

    Article  PubMed  CAS  Google Scholar 

  • Herrin DL, Schmidt GW (1989) Self-splicing of the chloroplast 23S intron of Chlamydomonas. Plant Physiol 89: 192s

    Article  Google Scholar 

  • Hildebrand M, Hallick RB, Passavant CW, Bourque DP (1988) Trans-splicing in chloroplasts: The rps12 loci of Nicotiana tabacum. Proc Natl Acad Sci USA 85: 372–376

    Article  PubMed  CAS  Google Scholar 

  • Hiratsuka J, Shimada H, Whittier R, Ishibashi T, Sakamoto M, Mori M, Kondo C, Honji Y, Sun C-R, Meng B-Y, Li Y-Q, Kanno A, Nishizawa Y, Hirai A, Shinozaki K, Sugiura M (1989) The complete sequence of the rice (Oryza sativa) chloroplast genome: intermolecular recombination between distinct tRNA genes accounts for a major plastid DNA inversion during the evolution of the cereals. Mol Gen Genet 217:185–194

    Article  PubMed  CAS  Google Scholar 

  • Hosier JP, Wurtz EA, Harris EH, Gillham NW, Boynton JE (1989) The relationship between plastid gene dosage and gene expression in the chloroplast of Chlamydomonas reinhardtii. Plant Physiol 91: 648–655

    Article  Google Scholar 

  • Keegstra K, Olsen LJ, Theg SM (1989) Chloroplastic precursors and their transport across the envelope membranes. Ann Rev Plant Physiol Plant Mol Biol 40: 471–501

    Article  CAS  Google Scholar 

  • Kindle KL, Schnell RA, Fernandez E, Lefebvre PA (1989) Stable nuclear transformation of Chlamydomonas using the Chlamydomonas gene for nitrate reductase. J Cell Biol 109: 2589–2601

    Article  PubMed  CAS  Google Scholar 

  • Kuchka MR, Mayfield SP, Rochaix J-D (1988) Nuclear mutations specifically affect the synthesis and/or degradation of the chloroplast-encoded D2 polypeptide of photosystem II in Chlamydomonas reinhardtii. EMBO J 7: 319–324

    PubMed  CAS  Google Scholar 

  • Kuchka MR, Goldschmidt-Clermont M, van Dillewijn J, Rochaix J-D (1989) Mutation at the Chlamydomonas nuclear NAC2 locus specifically affects stability of the chloroplast psbD transcript encoding polypeptide D2 of PS II. Cell 58: 869–876

    Article  PubMed  CAS  Google Scholar 

  • Lansman RA, Clayton DA (1975) Mitochondrial protein synthesis in mouse L-cells: Effect of selective nicking of mitochondrial DNA. J Mol Biol 99:777–793

    Article  PubMed  CAS  Google Scholar 

  • Lemieux C, Boulanger J, Otis C, Turmel M (1989) Nucleotide sequence of the chloroplast large subunit rRNA gene from Chlamydomonas reinhardtii. Nucl Acids Res 17:7997

    Article  PubMed  CAS  Google Scholar 

  • Liu X-Q, Gillham NW, Boynton JE (1988) Chloroplast ribosomal protein L-18 in Chlamydomonas reinhardtii is processed during ribosome assembly. Mol Gen Genet 214: 588–591

    Article  PubMed  CAS  Google Scholar 

  • Liu X-Q, Gillham NW, Boynton JE (1989a) Chloroplast ribosomal protein gene rpsll of Chlamydomonas reinhardtii. Wild-type sequence, mutation to streptomycin resistance and dependence, and function in Escherichia coli. J Biol Chem 264: 16100–16108

    PubMed  CAS  Google Scholar 

  • Liu X-Q, Hosier JP, Boynton JE, Gillham NW (1989b) mRNAs for two ribosomal proteins are preferentially translated in the chloroplast of Chlamydomonas reinhardtii under conditions of reduced protein synthesis. Plant Mol Biol 12: 385–394

    Article  CAS  Google Scholar 

  • Lou JK, Wu M, Chang CH, Cuticchia AJ (1987) Localization of a r-protein gene within the chloroplast DNA replication origin of Chlamydomonas. Curr Genet 11:537–541

    Article  PubMed  CAS  Google Scholar 

  • Lou JK, Cruz FD, Wu M (1989) Nucleotide sequence of the chloroplast ribosomal protein gene L14 in Chlamydomonas reinhardtii. Nucl Acids Res 17: 3587

    Article  PubMed  CAS  Google Scholar 

  • Makosky PC, Dahlberg AE (1987) Spectinomycin resistance at site 1192 in 16S ribosomal RNA of E. coli: an analysis of three mutants. Biochimie 69: 885–889

    Article  PubMed  CAS  Google Scholar 

  • Mayfield SP, Kindle KL (1990) Stable nuclear transformation of Chlamydomonas reinhardtii by using a C. reinhardtii gene as the selectable marker. Proc Natl Acad Sci USA 87: 2087–2091

    Article  PubMed  CAS  Google Scholar 

  • Melançon P, Lemieux C, Brakier-Gingras L (1988) A mutation in the 530 loop of Escherichia coli 16S ribosomal RNA causes resistance to streptomycin. Nucl Acids Res 16:9631–9639

    Article  PubMed  Google Scholar 

  • Moazed D, Noller HF (1987) Interaction of antibiotics with functional sites in 16S ribosomal RNA. Nature 327: 389–394

    Article  PubMed  CAS  Google Scholar 

  • Montandon P-E, Nicolas P, Schürmann P, Stutz E (1985) Streptomycin-resistance of Euglena gracilis chloroplasts: identification of a point mutation in the 16S rRNA gene in an invariant position. Nucl Acids Res 13: 4299–4310

    Article  PubMed  CAS  Google Scholar 

  • Montandon P-E, Wagner R, Stutz E (1986) E. coli ribosomes with a C912 to U base change in the 16S rRNA are streptomycin resistant. EMBO J 5: 3705–3708

    PubMed  CAS  Google Scholar 

  • Myers AM, Harris EH, Gillham NW, Boynton JE (1984) Mutations in a nuclear gene of Chlamydomonas cause the loss of two chloroplast ribosomal proteins, one synthesized in the chloroplast and the other in the cytoplasm. Curr Genet 8: 369–378

    Article  CAS  Google Scholar 

  • Newman SM, Boynton JE, Gillham NW, Randolph-Anderson BL, Johnson AM, Harris EH (1990) Transformation of chloroplast ribosomal RNA genes in Chlamydomonas: Molecular and genetic characterization of integration events. Genetics, in press.

    Google Scholar 

  • Ghyama K, Fukuzawa H, Kohchi T, Shirai H, Sano T, Sano S, Umesono K, Shiki Y, Takeuchi M, Chang Z, Aota S, Inokuchi H, Ozeki H (1986) Chloroplast gene organization deduced from complete sequence of liverwort Marchanda polymorpha chloroplast DNA. Nature 322: 572–574

    Article  Google Scholar 

  • Randolph-Anderson BL, Boynton JE, Gillham NW (1989) Electfophoretic and immunological comparisons of chloroplast and prokaryotic ribosomal proteins reveal that certain families of large subunit proteins are evolutionarily conserved. J Mol Evol 29: 68–88

    Article  PubMed  CAS  Google Scholar 

  • Raué HA, Klootwijk J, Musters W (1988) Evolutionary conservation of structure and function of high molecular weight ribosomal RNA. Prog Biophys Mol Biol 51:77–129

    Article  PubMed  Google Scholar 

  • Robertson D, Boynton JE, Gillham NW (1990) Cotranscription of the wild type chloroplast atpE gene encoding the CF1/CF0 epsilon subunit with the 3’ half of the rps7 gene in Chlamydomonas reinhardtii and characterization of frameshift mutations in atpE. Mol Gen Genet 221:155–163

    Article  PubMed  CAS  Google Scholar 

  • Rochaix J-D, Rahire M, Michel F (1985) The chloroplast ribosomal intron of Chlamydomonas reinhardii codes for a polypeptide related to mitochondrial maturases. Nucl Acids Res 13:975–984

    Article  PubMed  CAS  Google Scholar 

  • Rochaix J-D, Kuchka M, Mayfield S, Schirmer-Rahire M, Girard-Bascou J, Bennoun P (1989) Nuclear and chloroplast mutations affect the synthesis or stability of the chloroplast psbC gene product in Chlamydomonas reinhardtii. EMBO J 8:1013–1021

    PubMed  CAS  Google Scholar 

  • Schmidt RJ, Richardson CB, Gillham NW, Boynton JE (1983) Sites of synthesis of chloroplast ribosomal proteins in Chlamydomonas, J Cell Biol 96:1451–1463

    Article  PubMed  CAS  Google Scholar 

  • Schmidt RJ, Myers AM, Gillham NW, Boynton JE (1984a) Chloroplast ribosomal proteins of Chlamydomonas synthesized in the cytoplasm are made as precursors. J Cell Biol 98: 2011–2018

    Article  PubMed  CAS  Google Scholar 

  • Schmidt RJ, Myers AM, Gillham NW, Boynton JE (1984b) Immunological similarities between specific chloroplast ribosomal proteins from Chlamydomonas reinhardtii and ribosomal proteins from E. coli. Mol Biol Evol 1: 317–334

    PubMed  CAS  Google Scholar 

  • Schmidt RJ, Hosier JP, Gillham NW, Boynton, JE (1985a) Biogenesis and evolution of chloroplast ribosomes: cooperation of nuclear and chloroplast genes. In Steinback KE, Bonitz S, Arntzen CJ, Bogorad L, (eds) Molecular Biology of the Photosynthetic Apparatus, Cold Spring Harbor Laboratory, pp. 417–427

    Google Scholar 

  • Schmidt RJ, Gillham NW, Boynton JE (1985b) Processing of the precursor to a chloroplast ribosomal protein made in the cytosol occurs in two steps, one of which depends on a protein made in the chloroplast. Mol Cell Biol 5: 1093–1099

    PubMed  CAS  Google Scholar 

  • Shinozaki K, Ohme M, Tanaka M, Wakasugi T, Hayashida N, Matsubayashi T, Zaita N, Chunwongse J, Obokata J, Yamaguchi-Shinozaki K, Ohto C, Torazawa K, Meng BY, Sugita M, Deno H, Kamogashira T, Yamada K, Kusuda J, Takaiwa F, Kato A, Tohdoh N, Shimada H, Sugiura M (1986) The complete nucleotide sequence of the tobacco chloroplast genome: its gene organization and expression. EMBO J 5: 2043–2049

    PubMed  CAS  Google Scholar 

  • Sigmund CD, Ettayebi M, Morgan EA (1984) Antibiotic resistance mutations in 16S and 23S ribosomal RNA genes of Escherichia coli. Nucl Acids Res 12:4653–4663

    Article  PubMed  CAS  Google Scholar 

  • Sor F, Fukuhara H (1982) Identification of two erythromycin resistance mutations in the mitochondrial gene coding for the large ribosomal RNA in yeast. Nucl Acids Res 10: 6571–6577

    Article  PubMed  CAS  Google Scholar 

  • Sor F, Fukuhara H (1984) Erythromycin and spiramycin resistance mutations of yeast mitochondria: nature of the rib2 locus in the large ribosomal RNA gene. Nucl Acids Res 12: 8313–8318

    Article  PubMed  CAS  Google Scholar 

  • Stern DB, Gruissem W (1987) Control of plastid gene expression: 3’ inverted repeats act as mRNA processing and stabilizing elements, but do not terminate transcription. Cell 51: 1145–1157

    Article  PubMed  CAS  Google Scholar 

  • Stern DB, Jones H, Gruissem W (1989) Function of plastid mRNA 3’ inverted repeats. RNA stabilization and gene-specific protein binding. J Biol Chem 264: 18742–18750

    PubMed  CAS  Google Scholar 

  • Stern S, Weiser B, Noller HF (1988) Model for the three-dimensional folding of 16 S ribosomal RNA. J Mol Biol 204: 447–481

    Article  PubMed  CAS  Google Scholar 

  • Tanaka M, Wakasugi T, Sugita M, Shinozaki K, Sugiura M (1986) Genes for the eight ribosomal proteins are clustered on the chloroplast genome of tobacco (Nicotiana tabacwn): Similarity to the S10 and spc operons of Escherichia coli. Proc Natl Acad Sci USA 83: 6030–6034

    Article  PubMed  CAS  Google Scholar 

  • van Acken U (1975) Protein chemical studies on ribosomal proteins S4 and S12 from ram (ribosomal ambiguity) mutants of Escherichia coli. Mol Gen Genet 140:61–68

    Article  PubMed  Google Scholar 

  • Walbot V, Coe EH Jr (1979) Nuclear gene iojap conditions a programmed change to ribosome-less plastids in Zea mays. Proc Natl Acad Sci USA 76: 2760–2764

    Article  PubMed  CAS  Google Scholar 

  • Watson JC, Surzycki SJ (1983) Both the chloroplast and nuclear genomes of Chlamydomonas reinhardi share homology with Escherichia coli genes for transcriptional and translations components. Curr Genet 7: 201–210

    Article  CAS  Google Scholar 

  • Wurtz EA, Boynton JE, Gillham NW (1977) Perturbation of chloroplast DNA amounts and chloroplast gene transmission in Chlamydomonas reinhardtii by 5-fluorodeoxyuridine. Proc Nati Acad Sci USA 74: 4552–4556

    Article  CAS  Google Scholar 

  • Zaita N, Torazawa K, Shinozaki K, Sugiura M (1987) Trans splicing in vivo: joining of transcripts from the “divided” gene for ribosomal protein S12 in the chloroplasts of tobacco. FEBS Lett 210:153–156

    Article  CAS  Google Scholar 

  • Zhou D-X, Quigley F, Massenet O, Mache R (1989) Cotranscription of the S10- and spc- like operons in spinach chloroplasts and identification of three of their gene products. Mol Gen Genet 216:439–445

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Departments of Zoology, Duke University, Durham, NC, 27706, USA

    N. W. Gillham & K. B. McElwain

  2. Departments of Botany, Duke University, Durham, NC, 27706, USA

    E. H. Harris, B. L. Randolph-Anderson, J. E. Boynton, C. R. Hauser & S. M. Newman

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  1. N. W. Gillham
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  2. E. H. Harris
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  3. B. L. Randolph-Anderson
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  4. J. E. Boynton
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  5. C. R. Hauser
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  6. K. B. McElwain
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  7. S. M. Newman
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Editors and Affiliations

  1. Laboratoire de Biologie Moléculaire Végétale, Université Joseph Fourier, B.P.53X, 38041, Grenoble cedex, France

    R. Mache

  2. Laboratoire de Biochimie Végétale, Université de Neuchâtel, CH-2007, Neuchâtel, Switzerland

    E. Stutz

  3. Max-Planck-Institut für Molekulare Genetik, Ihnestrasse 73, W-1000, Berlin 33, Germany

    A. R. Subramanian

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© 1991 Springer-Verlag Berlin Heidelberg

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Gillham, N.W. et al. (1991). Molecular Genetics of Chloroplast Ribosomes In Chlamydomonas . In: Mache, R., Stutz, E., Subramanian, A.R. (eds) The Translational Apparatus of Photosynthetic Organelles. NATO ASI Series, vol 55. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-75145-5_11

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  • DOI: https://doi.org/10.1007/978-3-642-75145-5_11

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