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Somatic Cell Hybridization by Protoplast Fusion and Morphogenesis

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Plant Tissue Culture and Its Bio-technological Application

Part of the book series: Proceedings in Life Sciences ((LIFE SCIENCES))

Abstract

Classic genetics began with higher plants. The experiments by Gregor Mendel with garden peas before the turn of the century led to the formulations of the laws of genetic inheritance (78). Basic research in biology was performed with microorganisms, which provided mono-ploids, and have a short generation time. The research has been extremely fruitful and has produced the modern concepts of molecular genetics and most recently a methodology of molecular cloning for gene analysis (13, 78, 82).

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References

  1. Anonymous: Isolation and culture of rice protoplasts. Vol. XVIII No. 6 in Scientia Sinica 18 (6), 779–784

    Google Scholar 

  2. Ahkong, Q.F., Fisher, D., Tompion, W., Lucy, J.A.: Mechanisms of cell fusion. Nature (London) 253, 194–195 (1975)

    Article  CAS  Google Scholar 

  3. Bates, L.S., Deyoe, C.W.: Wide hybridization and cereal improvement. Econ. Botany 27, 401–412 (1973)

    Article  Google Scholar 

  4. Bonnett, H.T., Eriksson, T.: Transfer of algal chloroplasts into protoplasts of higher plants. Planta (Berl.) 120, 71–79 (1974)

    Article  CAS  Google Scholar 

  5. Borlaug, N.E.: The green revolution, peace and humanity. Cereal Sci. Today 16, 401–411 (1971)

    Google Scholar 

  6. Broertjies, C., Roest, S., Bokelmann, G.S.: Mutation breeding of Chrysanthemum morifolium. Ram. using in vivo and in vitro adventitious bud techniques. Euphytica 25, 11–19 (1976)

    Article  Google Scholar 

  7. Brown, A.W.A., Byerly, T.C., Gibbs, M., San Pietro, A.: Crop Productivity -Research Imperatives. Yellow Springs, Ohio: C.F. Kettering Foundation, 1975

    Google Scholar 

  8. Bui-Dang, Ha, D., MacKenzie, I.A.: Division of protoplasts from Asparagus officinalis L and their growth and differentiation. Protoplasma 78, 215–221 (1973)

    Article  Google Scholar 

  9. Burgess, J., Fleming, E.N.: Ultrastructural studies of aggregation and fusion of plant protoplasts. Planta (Berl.) 118, 183–193 (1974)

    Article  CAS  Google Scholar 

  10. Chaleff, R.S., Carlson, P.S.: Somatic cell genetics of higher plants. Annu. Rev. Genet. 8, 267–278 (1974)

    Article  PubMed  CAS  Google Scholar 

  11. Cocking, E.C.: Plant cell protoplasts — isolation and development. Ann. Rev. Plant Physiol. 23, 29–50 (1972)

    Article  CAS  Google Scholar 

  12. Cocking, E.C.: A new procedure for the selection of somatic hybrids in plants. In: Proc. Cell Genetics in Higher Plants. Szeged, Hungary: Biological Res. Cr., 1976

    Google Scholar 

  13. Cohen, S.N., Chang, C.Y.: A method for selective cloning of Eukaryotic DNA fragments in E. coli by repeated transformation. Mol. Gen. Genet. 134, 133–141 (1974)

    Article  PubMed  CAS  Google Scholar 

  14. Constabel, F., Kirkpatrick, J.W., Kao, K.N., Kartha, K.K.: The effect of can-avanine on the growth of cells from suspension cultures and on the intergen-eric heterokaryocytes of canavanine sensitive and tolerant plants. Biochem. Physiol. Pflanzen 168, 319–325 (1975)

    CAS  Google Scholar 

  15. Constabel, F., Dudits, D., Gamborg, O.L., Kao, K.N.: Nuclear fusion in inter-generic heterokaryons. Can. J. Botany 53, 2092–2095 (1975)

    Article  Google Scholar 

  16. Constabel, F.: Isolation and culture of plant protoplasts. In: Plant Tissue Culture Methods. Gamborg, O.L., Wetter, L.R. (eds.). Ottawa: Nat. Res. Coun. Can., 1975, pp. 11–21

    Google Scholar 

  17. Constabel, F., Weber, G., Kirkpatrick, J.W., Pahl, K.: Cell division of inter-generic protoplast fusion products. Z. Pflanzenphysiol. 79, 1–7 (1976)

    Google Scholar 

  18. Coutts, R.H.A., Wood, K.R.: Isolation and culture of cucumber mesophyll protoplasts. Plant Sci. Lett. 4, 189–193 (1975)

    Article  Google Scholar 

  19. Davey, M.R., Bush, E., Power, J.B.: Cultural studies of a dividing legume leaf protoplast system. Plant Sci. Lett. 3, 127–133 (1974)

    Article  Google Scholar 

  20. Davidson, R.L.: Regulation of gene expression in somatic cell hybrids a Review. In Vitro 6, 411–426 (1971)

    Article  PubMed  CAS  Google Scholar 

  21. Davidson, R.L., Gerald, P.S.: Improved techniques for the induction of mammalian cell hybridization by polyethylene glycol. Somatic Cell Gen. 2, 165–176 (1976)

    Article  CAS  Google Scholar 

  22. Dorion, N., Chupeau, Y., Bourgin, J.P.: Isolation, culture and regeneration into plants of Ranunculus scelevatus L leaf protoplasts. Plant Sci. Lett. 5, 325–311 (1975)

    Article  Google Scholar 

  23. Dudits, D., Kao, K.N., Constabel, F., Gamborg, O.L.: Embryogenesis and formation of tetraploid and hexaploid plants from carrot protoplasts. Can. J. Botany 54, 1063–1064 (1976)

    Article  CAS  Google Scholar 

  24. Dudits, D., Rasko, I., Kadlaczky, Gy., Lima-de-Faria, A.: Fusion of human cells with Daucus carota protoplasts by polyethylene glycol. Hereditas 82, 121–214 (1976)

    Article  PubMed  CAS  Google Scholar 

  25. Dudits, D., Kao, K.N., Constabel, F., Gamborg, O.L.: Fusion of carrot and barley protoplasts and division of heterokaryocytes. Can. J. Genet. Cytol. 18, 263–269 (1976)

    Google Scholar 

  26. Durand, J., Potrykus, I., Donn, G.: Plants from protoplasts of Petunia. Z. Pflanzenphysiol. 69, 26–34 (1973)

    CAS  Google Scholar 

  27. Eriksson, T.: Isolation and Fusion of Plant Protoplasts. Colloq. Inter. C. N. R. S. (Paris) 193, 297–302 (1969)

    Google Scholar 

  28. Eriksson, T., Bonnett, H., Glimelius, K., Wallin, A.: Technical advances in protoplast isolation, culture and fusion. In: Tissue Culture and Plant Science. Street, H.E. (ed.). New York: Academic Press, 1974, pp. 213–231

    Google Scholar 

  29. Ferenczy, L., Kevei, F., Szegedi, M.: High-frequency fusion of fungal protoplasts. Experientia 31, 1028–1030 (1975)

    Article  CAS  Google Scholar 

  30. Fowke, L.C., Bech-Hansen, C.W., Gamborg, O.L., Shyluk, J.P.: Electron microscopic observations of cultured cells and protoplasts of Ammi visnaga. Am. J. Botany 60, 304–312 (1973)

    Article  Google Scholar 

  31. Fowke, L.C., Bech-Hansen, C.W., Gamborg, O.L., Constabel, F.: Electron microscopic observations of mitosis and cytokinesis in multinucleate protoplasts of soybean. J. Cell. Sci. 18, 491–507 (1975)

    PubMed  CAS  Google Scholar 

  32. Fowke, L.C., Rennie, P.J., Kirkpatrick, J.W., Constabel, F.: Ultrastructural characteristics of intergeneric protoplast fusion. Can. J. Botany 52, 272–278 (1975)

    Google Scholar 

  33. Fowke, L.C., Rennie, P.J., Kirkpatrick, J.W., Constabel, F.: Ultrastructural characterization of soybean x sweet clover fusion products. Planta (Berl.) 130, 39–45 (1976)

    Article  Google Scholar 

  34. Gamborg, O.L.: Advances in somatic cell hybridization in higher plants. In: Stadler Symposium. Columbia: Univ. Missouri, 1975, Vol. VII, pp. 34–46

    Google Scholar 

  35. Gamborg, O.L.: Culture media for plant protoplasts. Contribution to: CRC Handbook of Nutrition and Food. Cleveland: CRC Press, Inc., 1976

    Google Scholar 

  36. Gamborg, O.L.: Plant Protoplasts, isolation, culture and fusion. In: Proc. Workshop on Cell Genetics in Higher Plants. Szeged, Hungary: (ICRO, UNESCO/ UNDP) Biological Research Center, 1976 pp. 107–127

    Google Scholar 

  37. Gamborg, O.L., Miller, R.A.: Isolation, culture, and uses of plant protoplasts. Can. J. Botany 51, 1795–1799 (1973)

    Article  Google Scholar 

  38. Gamborg, O.L., Shyluk, J.P.: Tissue Culture, protoplasts and morphogenesis in Flax. Botan. Gaz. 137, 301–306 (1976)

    Article  Google Scholar 

  39. Gamborg, O.L., Wetter, L.R. (eds.): Plant Tissue Culture Methods. Saskatoon: Nat. Res. Coun. Canada, 1975, pp. 109

    Google Scholar 

  40. Gamborg, O.L., Shyluk, J., Kartha, K.K.: Factors affecting the isolation and callus formation in protoplasts from the shoot apices of Pisum sativum. Plant Sci. Lett. 4, 285–292 (1975)

    Article  CAS  Google Scholar 

  41. Gamborg, O.L., Constabel, F., Kao, K.N., Ohyama, K.: Plant protoplasts in genetic modification and production of intergeneric hybrids. In: Modification of the Information Content of Plant Cells. Proc. 2nd John Innes Sympos. R. Markham, D.R. Davies, D.A. Hapwood, R.W. Home (eds.). Amsterdam: North Holland, 1974, pp. 181–196

    Google Scholar 

  42. Gamborg, O.L., Kartha, K.K., Dudits, D.: Morphogenesis in somatic plant cells and protoplasts. In: Physiol. Biochem. Crop Plants. USSR, From Symposium on Growth and Development Int. Bot. Congress, Leningrad, 1975

    Google Scholar 

  43. Gamborg, O.L., Constabel, F., Fowke, L.C., Kao, K.N., Ohyama, K., Kartha, K.K., Pelcher, L.E.: Protoplast and cell culture methods in somatic hybridization in higher plants. Can. J. Genet. Cytol. 16, 737–750 (1974)

    Google Scholar 

  44. Gleba, Y.Y., Butenko, R.G., Sytnik, K.M.: Fusion of protoplasts and parasexual hybridization in Nicotiana tabacum. Dokl. Akad. Nauk USSR. 221, 1196–1198 (1975)

    Google Scholar 

  45. Glimelius, K., Wallin, A., Eriksson, T.: Agglutinating effects of concanavalin A on isolated protoplasts of Daucus carota. Physiol. Plantarum 31, 225–230 (1974)

    Article  CAS  Google Scholar 

  46. Gosch, G., Bajaj, Y.P.S., Reinert, J.: Isolation, culture and induction of embryogenesis in protoplasts from cell suspensions of Atropa belladonna. Protoplasma 86, 405–410 (1975)

    Article  CAS  Google Scholar 

  47. Grambow, H.J., Kao, K.N., Miller, R.A., Gamborg, O.L.: Cell division and plant development from protoplasts of carrot cell suspension cultures. Planta (Berlin.) 103, 348–355 (1972)

    Article  Google Scholar 

  48. Hartmann, J.X., Kao, K.N., Gamborg, O.L., Miller, R.A.: Immunological methods for the agglutination of protoplasts from cell suspension cultures of different genera. Planta (Berl.) 112, 45–56 (1973)

    Article  CAS  Google Scholar 

  49. Hess, D.: Transformation an höheren Organismen. Naturwissenschaften 59, 348–355 (1972)

    Article  PubMed  CAS  Google Scholar 

  50. Holl, F.B.: Innovative approaches to genetics in agriculture. Can. J. Genet. Cytol. 17, 517–524 (1975)

    Google Scholar 

  51. Holl, F.B.: Molecular genetic modification of legumes. In: Molecular Genetic Modification of Eukaryotes. Rubenstein, I. (ed.). New York: Academic Press, 1976

    Google Scholar 

  52. Holl, F.B., Gamborg, O.L., Ohyama, K., Pelcher, L.E.: Genetic transformation in plants. In: Tissue Culand Plant Science. Street, H.E. (ed.). New York: Academic Press, 1974, pp. 310–327

    Google Scholar 

  53. Ito, M., Maeda, M.: Meiotic division and fusion of nuclei in multinucleate cells from induced fusion of meiotic protoplasts of Liliaceous plants. Botan. Mag. (Tokyo) 87, 219–228 (1974)

    Article  Google Scholar 

  54. Jones, L.H.: Plant cell culture and biochemistry: studies for improved vegetable oil production. In: Industrial Aspects of Biochemistry. Spencer, B. (ed.). North-Holland/American Elsevier, Amsterdam-New York (1974), Vol. 30, Part II, pp. 813–833

    Google Scholar 

  55. Keller, W.A., Melchers, G.: Effect of high pH and calcium on tobacco leaf protoplast fusion. Z. Naturforsch. 28C, 737–741 (1973)

    Google Scholar 

  56. Kao, K.N.: Cytological studies on plant heterokaryocytes — nuclear behavior. In: Workshop on Cell Genetics in Higher Plants. Hungary: Szeged, 1976

    Google Scholar 

  57. Kao, K.N., Michayluk, M.R.: A method for high frequency intergeneric fusion of plant protoplasts. Planta (Berl.) 115, 355–367 (1974)

    Article  CAS  Google Scholar 

  58. Kao, K.N., Michayluk, M.R.: Nutritional requirements for growth of Vicia hajastana cells and protoplast at a very low population density in liquid media. Planta (Berl.) 126, 105–110 (1975)

    Article  CAS  Google Scholar 

  59. Kao, K.N., Constabel, F., Michayluk, M.R., Gamborg, O.L.: Plant protoplast fusion and growth of intergeneric hybrid cells. Planta (Berl.) 120, 215–227 (1974)

    Article  CAS  Google Scholar 

  60. Kao, K.N., Gamborg, O.L., Michayluk, M.R., Keller, W.A.: Effects of sugars and inorganic salts on cell regeneration and sustained division in plant protoplasts. Colloq. Intern. C.N.R.S. 212, 207–213 (1973)

    Google Scholar 

  61. Kao, K.N., Keller, W.A., Miller, R.A.: Cell division in newly formed cells from protoplasts of soybean. Exp. Cell Res. 62, 338–340 (1970)

    Article  PubMed  CAS  Google Scholar 

  62. Kartha, K.K., Gamborg, O.L.: Potential value of a tissue culture technique for producing mosaic disease-free cassava plants. IDRC Monograph -049e, “The international exchange and testing of cassava germ plasm: proceedings of an interdisciplinary workshop held at CIAT, Palmira, Columbia, 4–6 February 1975.” Nestel, B., Maclntyre, R. (eds.). Ottawa: Intern. Devel. Rese. Ctr., 1975

    Google Scholar 

  63. Kartha, K.K., Gamborg, O.L., Constabel, F., Kao, K.N.: Fusion of rapeseed and soybean protoplasts and subsequent division of heterokaryocytes. Can. J. Botany 52, 2435–2436 (1974)

    Article  Google Scholar 

  64. Kartha, K.K., Michayluk, M.R., Kao, K.N., Gamborg, O.L.: Callus formation and plant regeneration from mesophyll protoplasts of rape plants (Brassica napus L. cv. Zephyr). Plant Sci. Lett. 3, 265–271 (1974)

    Article  CAS  Google Scholar 

  65. Kasha, K.J.: Haploids in Higher Plants. Guelph: Univ. Guelph, 1974, pp.421

    Google Scholar 

  66. Krishnamurthi, M.: Isolation, fusion and multiplication of sugarcane protoplasts and comparison of sexual and parasexual hybridization. Euphytica 25, 145–150 (1976)

    Article  Google Scholar 

  67. Küster, E.: Über die Verschmelzung nackter Protoplasten. Ber. Deut. Botan. Ges. 27, 589–598 (1909)

    Google Scholar 

  68. Ledoux, L.: Genetic Manipulations with Plant Material. NATO Advanced Study Institutes. New York: Plenum Press, 1975, Vol. III

    Google Scholar 

  69. Melchers, G., Labib, G.: Somatic hybridization of plants by fusion of protoplasts. Mol. Gen. Genet. 135, 277–294 (1974)

    Article  Google Scholar 

  70. Miller, R.A., Gamborg, O.L., Keller, W.A., Kao, K.N.: Fusion and division of nuclei in multinucleated soybean protoplasts. Can. J. Genet. Cytol. 13, 347–353 (1971)

    Google Scholar 

  71. Misawa, M., Sakato, K., Tanaka, H., Hayaski, M., Samejima, H.: Production of physiologically active substances by plant cell suspension cultures. In: Tissue Culture and Plant Science. Street, H.E. (ed.). New York: Academic Press, 1974, pp. 405–432

    Google Scholar 

  72. Murashige, T.: Plant propagation through tissue cultures. Ann. Rev. Plant Physiol. 25, 135–166 (1974)

    Article  CAS  Google Scholar 

  73. Nawa, S., Yamada, M., Ohta, Y.: Hereditary changes in Capsicum annum L. induced by DNA treatment. Japan J. Genet. 50, 341–344 (1975)

    Article  Google Scholar 

  74. Nickell, L.G., Torrey, J.G.: Crop improvement through plant cell and tissue culture. Science 166, 1068–1070 (1969)

    Article  PubMed  CAS  Google Scholar 

  75. Ohyama, K., Gamborg, O.L., Miller, R.A.: Uptake of exogenous DNA by plant protoplasts. Can. J. Botany 50, 2077–2080 (1972)

    Article  CAS  Google Scholar 

  76. Pelcher, L.E., Kool, A.: Unpubl.

    Google Scholar 

  77. Pelcher, L.E., Gamborg, O.L., Kao, K.N.: Bean mesophyll protoplasts — production, culture and callus formation. Plant Sci. Lett. 3, 107–111 (1974)

    Article  Google Scholar 

  78. Peters, J.A.: Classic Papers in Genetics. New Jersey: Prentice-Hall, 1959

    Google Scholar 

  79. Potrykus, I.: Uptake of cell organelles into isolated protoplasts. In: Modification of the information content of plant cells. Markham, R. (ed.). Amsterdam: North Holland, 1975, pp. 169–179

    Google Scholar 

  80. Puck, T.: Hybridization and somatic cell genetics. In: Somatic Cell Hybridization. Davidson, R.L., de La Cruz, F. (eds.). New York: Raven Press, 1974, pp. 27–34

    Google Scholar 

  81. Radsak, K., Sawicki, W., Koprowsk, H.: Fusion of isolated mitochondria with tissue culture cells. Z. Naturforsch. 27, 419 (1972)

    CAS  Google Scholar 

  82. Raspe, G.: Workshop on mechanisms and prospects of genetic exchange. Advan. Biosci. 8. New York: Pergamon Press (1972)

    Google Scholar 

  83. Reinhard, E.: Biotransformation by plant tissue cultures. In: Tissue Culture and Plant Science. Street, H.E. (ed.). New York: Academic Press, 1974, pp. 433–459

    Google Scholar 

  84. Ruddle, F.H., Kucherlapati, R.S.: Hybrid cells and human genes. Sci. Am. 23, 36–41 (1974)

    Article  Google Scholar 

  85. Schieder, O.: Selection of a somatic hybrid between auxotrophic mutants of Sphaerocarpos donnellii Aust. using the method of protoplast fusion. Z. Pflanzenphysiol. 74, 357–365 (1974)

    Google Scholar 

  86. Schieder, O.: Regeneration of haploid and diploid Datura innoxia mill — mesophyll protoplasts to plants. Z. Pflanzenphysiol. 76, 462–466 (1975)

    Google Scholar 

  87. Street, H.E.: Plant Tissue and Cell Culture. Botanical Monographs. London: Blackwell Sci., 1973, Vol. II

    Google Scholar 

  88. Strobel, G.A.: Biochemical basis of the resistance of sugarcane to eyespot disease. Proc. Natl. Acad. Sci. 70, 1693–1696 (1973)

    Article  PubMed  CAS  Google Scholar 

  89. Stumm, I., Meyer, Y., Abel, W.O.: Regeneration of the moss Physcomitrella patens (Hedw.) from isolated protoplasts. Plant Sci. Lett. 5, 113–118 (1975)

    Article  Google Scholar 

  90. Takebe, I., Labib, G., Melchers, G.: Regeneration of whole plants from isolation mesophyll protoplasts of tobacco. Naturwissenschaften 58, 318–320 (1971)

    Article  Google Scholar 

  91. Takebe, I.: The use of protoplasts in plant virology. Ann. Rev. Phytopath. 13, 105–125 (1975)

    Article  Google Scholar 

  92. Thomas, E., Hoffmann, F., Potrykus, I., Wenzel, G.: Protoplast regeneration and stem embryogenesis of haploid androgenetic rape. Mol. Gen. Genet. 154, 245–247 (1976)

    Article  Google Scholar 

  93. Tomasz, A.: DNA uptake by bacteria and enkaryotic cells. In: Stadler Genetics Symposia. Vol. 5. Kimber G., Redei, G.P. (eds.). Columbia: 1973, Vol. V, pp. 161–178

    Google Scholar 

  94. Turbin, N.V., Soyfer, V.N., Kartei, N.A., Chekalin, N.M., Dorohov, Y.L., Titov, Y.B., Cieminis, K.K.: Genetic modification of the waxy character in barley under the action of exogenous DNA of the wild variety. Mutation Res. 27, 59–68 (1975)

    Article  CAS  Google Scholar 

  95. Upadhya, M.D.: Isolation and culture of mesophyll protoplasts of potato (Solanum tuberosum L.). Potato Res. 18, 438–445 (1975)

    Article  Google Scholar 

  96. Vardi, A., Spiegelroy, P., Galun, E.: Citrus cell culture — isolation of protoplasts, plating densities, effect of mutagens and regeneration of embryos. Plant Sci. Lett. 4, 231–236 (1975)

    Article  Google Scholar 

  97. Vasil, I.K., Vasil, V.: Totipotency and Embryogenesis in Plant cell and tissue cultures. In Vitro 8, 117–127 (1972)

    Article  PubMed  CAS  Google Scholar 

  98. Vasil, I.K., Giles, K.L.: Induced transfer of higher plant chloroplasts into fungal protoplasts. Science 190, 680 (1975)

    Article  PubMed  CAS  Google Scholar 

  99. Vasil, I.K., Nitsch, C.: Experimental production of pollen haploids and their use. Z. Pflanzenphysiol. 76, 191–212 (1975)

    Google Scholar 

  100. Vasil, I.K.: The progress, problems, and prospects of plant protoplast research. Advan. Agron. 28, 121–160 (1976)

    Google Scholar 

  101. Wallin, A., Glimelius, K.G., Eriksson, T.: The induction of aggregation and fusion of Daucus carota protoplasts by polyethylene glycol. Z. Pflanzenphysiol. 74, 64–80 (1974)

    CAS  Google Scholar 

  102. Wetter, L.R., Kao, K.N.: The use of isoenzymes of distinguishing the sexual and somatic hybrids in callus cultures derived from Nicotiana. Z. Pflanzenphysiol. (1976)

    Google Scholar 

  103. Willecke, K., Ruddle, F.H.: Transfer of the human gene for hypoxanthine -guanine phosphoribosyltransferase via isolated human metaphase chromosomes into mouse L — cells. Proc. Natl. Acad. Sci. 72, 1792–1796 (1975)

    Article  PubMed  CAS  Google Scholar 

  104. Williamson, F.G., Fowke, L.C., Gamborg, O.L., Constabel, F.: Labelling of concanavalin-A sites on the plasma membrane of soybean protoplasts. Protoplasma 89, 305–316 (1976)

    Article  CAS  Google Scholar 

  105. Withers, L.A., Cocking, E.C.: Fine structural studies on spontaneous and induced fusion of higher plant protoplasts. J. Cell Sci. 11, 59–75 (1972)

    PubMed  CAS  Google Scholar 

  106. Zenk, M.H.: Haploids in physiological and biochemical research. In: Haploids in Higher Plants, Advances and Potential. Kasha, K.J. (ed.). Guelph: Univ. Guelph, 1974, pp. 339–353

    Google Scholar 

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Gamborg, O.L. (1977). Somatic Cell Hybridization by Protoplast Fusion and Morphogenesis. In: Barz, W., Reinhard, E., Zenk, M.H. (eds) Plant Tissue Culture and Its Bio-technological Application. Proceedings in Life Sciences. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-66646-9_24

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