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Improved techniques for the induction of mammalian cell hybridization by polyethylene glycol

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Somatic Cell Genetics

Abstract

Modifications in the techniques for the induction of mammalian somatic cell hybridization by polyethylene glycol (PEG) have led to procedures that are rapid, simple, and effective. The basic improvements, for both monolayer and suspension fusions, are a short exposure to PEG and a rapid dilution of PEG following treatment. There is a marked effect of PEG concentration on cell hybridization, and there seem to be inherent differences between cells in terms of the extent of cell fusion induced by PEG.

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Authors and Affiliations

  1. Division of Human Genetics, Children's Hospital Medical Center, 02115, Boston, Massachusetts

    Richard L. Davidson & Park S. Gerald

  2. Department of Microbiology and Molecular Genetics, Harvard Medical School, 02115, Boston, Massachusetts

    Richard L. Davidson

  3. Department of Pediatrics, Harvard Medical School, 02115, Boston, Massachusetts

    Park S. Gerald

Authors
  1. Richard L. Davidson
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  2. Park S. Gerald
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Davidson, R.L., Gerald, P.S. Improved techniques for the induction of mammalian cell hybridization by polyethylene glycol. Somat Cell Mol Genet 2, 165–176 (1976). https://doi.org/10.1007/BF01542629

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  • DOI: https://doi.org/10.1007/BF01542629

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