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Somatic hybridisation of plants by fusion of protoplasts

I. Selection of light resistant hybrids of “haploid” light sensitive varieties of tobacco

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Summary

Mesophyll protoplasts of “haploids” produced by “anther culture” of two chlorophyll deficient, light sensitive varieties of Nicotiana tabacum, sublethal (s) and virescent (v) are fused with the method of Keller and Melchers (1973). The double heterozygote hybrid of the two recessive genes for light sensitivity complements to normal leaf colour and resistance to high light intensity. By using this complementation obviously 12, possibly 21 independently arisen somatic hybrid plants are selected. Since leaf colour mutations are frequent and light sensitivity of leaf colour mutants is not too rare in higher plants, it is possible to use generally this selection method.

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Authors and Affiliations

  1. Max-Planck-Institut für Biologie, Tübingen

    Georg Melchers & Gudrun Labib

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  1. Georg Melchers
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  2. Gudrun Labib
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Communicated by H. Stubbe

This paper contains unusually rich illustrations. We thank the Springer-Verlag for this donation. Each coeditor of MGG should receive the same donation after 40 years of cooperation.—We dedicate this paper to the memory of the teachers of one of the authors (M), editors of this Journal in a hard time: Alfred Kühn † to his 90. birthday (22. 4. 75) and Fritz v. Wettstein † to his 80. birthday (24. 6. 75).—We hope that somatic genetics of animals and plants may deliver many interesting contributions to MGG in the future.

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Melchers, G., Labib, G. Somatic hybridisation of plants by fusion of protoplasts. Molec. Gen. Genet. 135, 277–294 (1974). https://doi.org/10.1007/BF00271144

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