Summary
The problems associated with the isolation of leaf protoplasts in sugarcanes have been overcome by the choice of spindle tissue as source material. It is now possible to isolate, fuse, regenerate and multiply sugarcane protoplasts. However, the usefulness is limited due to the shortlived chromosomal stability during the callus stage. Nevertheless, preliminary results are promising and the method should prove feasible with refinement in technique. Two breeding systems for using parasexual hybridization are discussed.
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References
Cocking, E. C., 1972. Plant cell protoplasts-isolation development. A. Rev. Pl. Physiol. 23: 29–50.
Carlson, P. S., H. H. Smith & R. D. Dearing, 1972. Parasexual interspecific plant hybridization. Proc. natn. Acad. Sci. USA 69: 2292–2294.
Gamborg, O. L., K. N. Kao & R. A. Miller, 1973. Cell regeneration, division and plant development from protoplasts. Colloques int. Cent. natn. Rech. scient. 212: 155–173.
Gleba, Y. Y., R. G. Butenko & L. G. Shvidleay, 1974. Plating of mesophyll protoplast, isolated from different photosynthetic mutants of Nicotiana tabacum L. (95). 3rd Int. Tissue Cult. Congr., Leicester, U.K.
Kao, K. N., O. L. Gamborg & R. A. Miller, 1973. The effects of sugar and inorganic salts on cell regeneration and sustained division in protoplasts. Coloques int. Cent. natn. Rech. scient 212: 207–213.
Kao, K. N., W. A. Keller, R. A. Miller, 1970. Cell division in newly formed cells from protoplasts of soyabean. Expl. Cell Res. 62: 338–340.
Kao, K. N. & M. R. Michayluk, 1974. A method for high frequency intergenetric fusion of plant protoplasts. Planta (Berl.) 115: 335–367.
Krishnamurthi, M. & J. Tlaskal, 1974. Fiji disease resistant Saccharum officinarum var. Pindar subclones from tissue culture. Proc. int. Soc. Sug. Cane Technol. 15: Plant Breeding Section 1–8.
Melchers, G. W. Keller & G. Labib, 1974. The consequences of the fusion of mesophyll protoplasts. 3rd Int. Tissue Cult. Congr., Leicester, UK.
Maretzki, A. & L. G. Nickell, 1973. Formation of protoplasts from sugarcane cell suspension and the regeneration of cell cultures from protoplasts. Colloques int. Cent. natn. Rech. scient. 212: 51–63.
Nagata, T. & I. Takebe, 1970. Cell wall regeneration and cell division in isolated tobacco mesophyll protoplasts. Planta (Berl.) 92: 300–308.
Power, J. B., S. E. Cumming & E. C. Cocking, 1970. Fusion of isolated protoplasts. Nature 225: 1016–1018.
Price, S., 1967. Interspecific hybridization in sugarcane breeding. Proc. int. Soc. Sug. Cane Technol. 12: 1021–1026.
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Krishnamurthi, M. Isolation, fusion and multiplication of sugarcane protoplasts and comparison of sexual and parasexual hybridization. Euphytica 25, 145–150 (1976). https://doi.org/10.1007/BF00041539
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DOI: https://doi.org/10.1007/BF00041539