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Strategies for Optimization of the Production of Rosmarinic Acid in Salvia officinalis L. and Salvia dolomitica Codd Biomass with Several Biotechnological Approaches

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Salvia Biotechnology

Abstract

Rosmarinic acid (RA) is a caffeic acid ester widely used by pharmaceutical and food industry. Its presence in several medicinal plant species and herb has been correlated with their biological activities and health beneficial effects. RA is widely used as additive to preserve foods, and because the chemical synthesis, even possible, remains time consuming and cost inefficient, there is a growing interest in development of biotechnological strategies to produce this compound. RA is abundant within the Lamiaceae family, particularly in plants belonging to genus Salvia. The use of plant tissues and cell cultures may represent an alternative strategy for the production of highly valuable plant metabolites and could be even adopted by pharmaceutical industries to develop new drugs and formulations. Protocols for shoots and calli regeneration, as well as cell cultures have been already established for Salvia officinalis and Salvia dolomitica with the aim to evaluate the content of RA in various tissues at different growth conditions. In particular, S. dolomitica Codd has been shown to be a good model for tissue culture studies due to its flexible propagation under in vitro conditions. The hydroxyphenylpyruvate reductase, SoHPPR, gene encoding a hydroxyphenylpyruvate reductase, a key biosynthetic enzyme of RA pathway, has been characterized in cell cultures of S. officinalis, representing a good target for metabolic engineering strategies.

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Abbreviations

2,4-d :

2,4-dichlorophenoxyacetic acid

4CL:

4-coumarate CoA ligase

BA:

6-benzyladenine

C4H:

Cinnamic acid 4-hydroxylase

CA:

Carnosic acid

CAR:

Carnosol

DW:

Dry weight

EO:

Essential oils

FW:

Fresh weight

HPPR:

Hydroxyphenylpyruvate reductase

HS:

Headspace

IAA:

Indol acetic acid

Kin:

Kinetin

MeJA:

Methyl jasmonate

PAL:

Phenylalanine ammonia lyase

PGR:

Plant growth regulators

RA:

Rosmarinic acid

RAS:

Rosmarinic acid synthase

SA:

Salicylic acid

SM:

Secondary metabolites

SoHPPR :

Salvia officinalis hydroxyphenylpyruvate reductase gene

TAT:

Tyrosine aminotransferase

VOCs:

Volatile organic compounds

ZEA:

Zeatin

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Authors and Affiliations

  1. CREA Research Centre for Vegetable and Ornamental Crops, Sanremo, Italy

    Marco Savona, Sara Barberini, Eric Mozzanini & Barbara Ruffoni

  2. CREA Research, Centre for Genomics and Bioinformatics, Montanaso Lombardo, Italy

    Laura Bassolino

  3. Department of Agrarian, Food and Agro-environmental Sciences, University of Pisa, Pisa, Italy

    Laura Pistelli

  4. Department of Pharmacy, University of Pisa, Pisa, Italy

    Luisa Pistelli

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  1. Marco Savona
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  2. Sara Barberini
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  3. Laura Bassolino
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  4. Eric Mozzanini
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  5. Laura Pistelli
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  6. Luisa Pistelli
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  7. Barbara Ruffoni
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Correspondence to Barbara Ruffoni .

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  1. Laboratory of Applied Biotechnology, The Stephan Angeloff Institute of Microbiology-BAS, Plovdiv, Bulgaria

    Vasil Georgiev

  2. Department of Analytical Chemistry, University of Food Technologies, Plovdiv, Bulgaria

    Atanas Pavlov

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Savona, M. et al. (2017). Strategies for Optimization of the Production of Rosmarinic Acid in Salvia officinalis L. and Salvia dolomitica Codd Biomass with Several Biotechnological Approaches. In: Georgiev, V., Pavlov, A. (eds) Salvia Biotechnology. Springer, Cham. https://doi.org/10.1007/978-3-319-73900-7_6

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