Abstract
Cell suspension cultures of Coleus blumei Benth. have been found to accumulate 8–11% of their dry weight as rosmarinic acid (α-O-caffeoyl-3,4-dihydroxyphenyl-lactic acid). Actively-growing tissue converts >20% of exogenously supplied phenylalanine and tyrosine to the caffeoyl ester and this high rate of synthesis coincides with an increase in phenylalanine ammonia-lyase specific activity. Administration to the cultures of known phenylpropanoid precursors of rosmarinic acid failed to enhance the latter's production and in some cases inhibited it.
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Abbreviations
- RA:
-
rosmarinic acid (α-O-caffeoyl-3,4-dihydroxyphenyllactic acid
- DOPA:
-
dihydroxyphenylalanine
- PAL:
-
phenylalanine ammonialyase
- DOPL:
-
dihydroxyphenyl-lactic acid
References
Braun, G., Seitz, U.: Verlauf der Akkumulation von Kaffee-, Ferula-und Chlorogensäure in Beziehung zur Cyanodinakkumulation bei 2 Zellinien von Daucus carota. Biochem. Physiol. Pflanzen 168, 93–100 (1975)
Camm, E.L., Towers, G.H.N.: Phenylalanine ammonia lyase, a review. Phytochemistry 12, 961–973 (1973)
Constabel, F., Gamborg, O.L., Kurz, W.G.W., Steck, W.: Production of secondary metabolites in plant cell cultures. Planta Med. 25, 158–165 (1974)
Danks, M.L., Fletcher, J.S., Rice, E.L.: Effects of phenolic inhibitors on growth and metabolism of glucose-UL-14C in Paul's Scarlet rose cell-suspension cultures. Amer. J. Bot. 62, 311–317 (1975)
Davies, M.E.: Effects of auxin on polyphenol accumulation and the development of phenylalanine ammonia lyase activity in dark grown suspension cultures of Paul's Scarlet rose. Planta (Berl.) 104, 66–77 (1972)
Döller, G., Alfermann, A.W. and Reinhard, E.: Produktion von Indolalkaloiden in Calluskulturen von Catharanthus roseus. Planta Med. 30, 14–20 (1976)
Ellis, B.E., Towers, G.H.N., Biogenesis of rosmarinic acid in Mentha. Biochem. J. 118, 291–297 (1970)
Ellis, B.E., Major, G., Zenk, M.H.: Preparation of L-tyrosine-ring-14C, L-DOPA-ring-14C and related metabolites. Anal. Biochem. 53, 470–477 (1973)
Gamborg, O.L.: Aromatic metabolism in plants V. The biosynthesis of chlorogenic acid and lignin in potato cell cultures. Can. J. Biochem. 45, 1451–1457 (1967)
Gamborg, O.L., Eveleigh, D.E.: Culture methods and detection of glucanases in suspension cultures of wheat and barley. Can. J. Biochem. 46, 417–421 (1968)
Hahlbrock, K., Wellmann, E.: Light-induced flavone biosynthesis and activity of phenylalanine ammonia-lyase and UDP-apiose synthetase in cell suspension cultures of Petroselinum hortense. Planta (Berl.) 94, 236–239 (1970)
Hahlbrock, K., Kuhlen, E., Lindl, T.: Änderungen von Enzymaktivitäten während des Wachstums von Zellsuspensionskulturen von Glycine max: Phenylalanin Ammonium-Lyase und p-Cumarate: CoA Ligase. Planta (Berl.) 99, 311–318 (1971)
Harborne, J.B.: Caffeic acid ester distribution in higher plants. Z. Naturforsch. 21b, 604–605 (1966)
Hiller, K., Kothe, N.: Chlorogen- und Rosmarinsäure—Vorkommen und quantitative Verteilung in Pflanzen der Saniculoideae. Pharmazie 22, 220–221 (1967)
Hirotani, M., Furuya, T.: Cardenolide synthesis in redifferentiated shoots from callus cultures of Digitalis purpurea. Phytochemistry 16, 610–611 (1977)
Li, H.C., Rice, E.L., Rohrbaugh, L.M., Wender, S.H.: Effects of abscisic acid on phenolic content and lignin biosynthesis in tobacco tissue culture. Physiol. Plant. 23, 928–936 (1970)
Litvinenko, V.I., Popova, T.P., Simonjon, A.V., Zoz, I.G., Sokolov, V.S.: “Gerbstoffe” und Oxyzimtsäureabkömmlinge in Labiaten. Planta Med. 27, 372–380 (1975)
Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randell, R.J.: Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193, 265–275 (1951)
Margna, U.: Control at the level of substrate supply—an alternative in the regulation of phenylpropanoid accumulation in plant cells. Phytochemistry 16, 419–426 (1977)
Rhodes, M.J.C., Wooltorton, L.S.C.: The enzymic conversion of hydroxy-cinnamic acids to p-coumarylquinic and chlorogenic acids in tomato fruits. Phytochemistry 15, 947–951 (1976)
Scarpati, M.L., Oriente, G.: Isolament e constituzione dell'acido rosmarinico (del Rosmarinus off.). Ricerca sci. 28, 2329–2333 (1958)
Staba, E.J.: Plant tissue culture as a technique for the phytochemist. In: Recent Advances in Phytochemistry vol. 2, pp. 75–106. Seikel, M.K., Runeckles, V.C., Eds. New York: Appleton-Century-Crofts, 1969
Stärk, D., Alfermann, A.W., Reinhard, E.: Verlauf von Phenylalanin-Ammonium-Lyase-Aktivität, Anthocyan- und Chlorogensäurebildung in verschiedenen Zellstämmen von Daucus carota. Planta Med. 30, 104–117 (1976)
Stickland, R.G., Sunderland, N.: Production of anthocyanins, flavonols and chlorogenic acids by cultured callus tissues of Haplopappus gracilis. Ann. Bot. 36, 443–457 (1972)
Stöckigt, J., Zenk, M.H.: Enzymatic synthesis of chlorogenic acid from caffeoyl CoA and quinic acid. FEBS Lett. 42, 131–134 (1974)
Widholm, J.M.: Control of aromatic amino acid biosynthesis in cultured plant tissues: effects of intermediates and aromatic amino acids on free levels. Physiol. Plant. 30, 13–18 (1974)
Widholm, J.M.: Selection and characterization of cultured carrot and tobacco lines resistant to lysine, methionine and proline analogues. Canad. J. Bot. 54, 1523–1529 (1976)
Zenk, M.H., El-Shagi, H., Schulte, V.: Anthraquinone production by cell suspension cultures of Morinda citrifolia. Planta Med. 1975 Suppl., 79–101 (1975)
Zucker, M.: Light and enzymes. Ann. Rev. Plant Physiol. 23, 133–156 (1972)
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Razzaque, A., Ellis, B.E. Rosmarinic acid production in Coleus cell cultures. Planta 137, 287–291 (1977). https://doi.org/10.1007/BF00388164
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DOI: https://doi.org/10.1007/BF00388164