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Molecular Imaging Using Nanobodies: A Case Study

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Single Domain Antibodies

Part of the book series: Methods in Molecular Biology ((MIMB,volume 911))

Abstract

Molecular imaging is a noninvasive method to measure specific biological processes in animal models and patients using imaging. In recent years there has been a tremendous evolution in hardware and software for imaging purposes. This progress has created an urgent need for new labeled targeted molecular probes. The unique physicochemical and pharmacokinetic properties of Nanobodies match the requirements of the ideal molecular imaging tracer. Preclinical studies show strong and specific targeting in vivo with rapid clearance of unbound probe resulting in high contrasted images at early time points after intravenous administration. These data suggest that the Nanobody platform might become a generic method for the development of next generation molecular imaging probes.

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Author information

Authors and Affiliations

  1. In Vivo Cellular and Molecular Imaging (ICMI) Laboratory, Vrije Universiteit Brussel (VUB), Brussels, Belgium

    Nick Devoogdt, Catarina Xavier, Sophie Hernot, Ilse Vaneycken, Matthias D’Huyvetter, Jens De Vos, Sam Massa, Vicky Caveliers & Tony Lahoutte

  2. Laboratory of Cellular and Molecular Immunology (CMIM), Vrije Universiteit Brussel (VUB), Brussels, Belgium

    Jens De Vos, Sam Massa & Patrick De Baetselier

  3. Department of Molecular and Cellular Interactions, Vlaams Instituut voor Biotechnologie (VIB), Brussels, Belgium

    Jens De Vos, Sam Massa & Patrick De Baetselier

  4. Department of nuclear Medicine, UZ Brussel, Brussels, Belgium

    Vicky Caveliers

  5. Nuclear Medicine Department, UZ Brussel, Brussels, Belgium

    Tony Lahoutte

Authors
  1. Nick Devoogdt
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  2. Catarina Xavier
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  3. Sophie Hernot
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  4. Ilse Vaneycken
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  5. Matthias D’Huyvetter
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  6. Jens De Vos
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  7. Sam Massa
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  8. Patrick De Baetselier
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  9. Vicky Caveliers
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  10. Tony Lahoutte
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Corresponding author

Correspondence to Tony Lahoutte .

Editor information

Editors and Affiliations

  1. , Molecular & Cellular Interactions, Vrije Universiteit Brussel, Pleinlaan 2, Brussels, 1050, Belgium

    Dirk Saerens

  2. , Molecular & Cellular Interactions, Vrije Universiteit Brussel, Pleinlaan 2, Brussels, 1050, Belgium

    Serge Muyldermans

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Devoogdt, N. et al. (2012). Molecular Imaging Using Nanobodies: A Case Study. In: Saerens, D., Muyldermans, S. (eds) Single Domain Antibodies. Methods in Molecular Biology, vol 911. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-968-6_35

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  • DOI: https://doi.org/10.1007/978-1-61779-968-6_35

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-967-9

  • Online ISBN: 978-1-61779-968-6

  • eBook Packages: Springer Protocols

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