Single Domain Antibodies

Methods and Protocols

  • Dirk Saerens
  • Serge Muyldermans

Part of the Methods in Molecular Biology book series (MIMB, volume 911)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Overview of Single Domain Antibodies

    1. Front Matter
      Pages 1-1
    2. Cécile Vincke, Serge Muyldermans
      Pages 15-26
  3. Single Domain Antibody Library Construction

    1. Front Matter
      Pages 37-37
    2. Olga Ignatovich, Laurent Jespers, Ian M. Tomlinson, Ruud M. T. de Wildt
      Pages 39-63
    3. Aurelien Olichon, Ario de Marco
      Pages 65-78
  4. Selection of Single Domain Antibodies

    1. Front Matter
      Pages 79-79
    2. Kristof De Schutter, Nico Callewaert
      Pages 125-134
    3. Mireille Pellis, Serge Muyldermans, Cécile Vincke
      Pages 135-150
    4. Tomoyuki Tanaka, Terence H. Rabbitts
      Pages 151-173
    5. Edward Dolk, Theo Verrips, Hans de Haard
      Pages 199-209
    6. Greg Hussack, Mehdi Arbabi-Ghahroudi, C. Roger MacKenzie, Jamshid Tanha
      Pages 211-239
    7. Rinse Klooster, Kim S. Rutgers, Silvère M. van der Maarel
      Pages 241-253
  5. Expression of Single Domain Antibodies and Derivatives

    1. Front Matter
      Pages 225-225

About this book


The development of the hybridoma technology created the possibility to obtain unlimited amounts of monoclonal antibodies (mAb) with high specificity and affinity for any target and to introduce mAbs in a wide range of applications; however, the bulky size of mAbs, costly production, and cumbersome engineering hampered regularly their streamlined development in some applications.  In Single Domain Antibodies: Methods and Protocols, expert researchers examine single variable domain antibody fragments, referred to as VH, VL, VHH or VNAR. These fragments are the smallest intact antigen-binding fragments that can be produced recombinantly at low cost.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Heavy chain antibodies Single variable domain V-NAR VHH sdAbs

Editors and affiliations

  • Dirk Saerens
    • 1
  • Serge Muyldermans
    • 2
  1. 1., Molecular & Cellular InteractionsVrije Universiteit BrusselBrusselsBelgium
  2. 2., Molecular & Cellular InteractionsVrije Universiteit BrusselBrusselsBelgium

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2012
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-967-9
  • Online ISBN 978-1-61779-968-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book