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Laser Capture Microdissection: Methods and Applications

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Laser Capture Microdissection

Part of the book series: Methods in Molecular Biology ((MIMB,volume 755))

Abstract

Laser microdissection is a nonmolecular, minimally disruptive method to obtain cytologically and/or phenotypically defined cells or groups of cells from heterogeneous tissues. It is a versatile technology and allows the preparation of homogenous isolates of specific subpopulations of cells from which RNA/DNA or protein can be extracted for RT-polymerase chain reaction (PCR), quantitative PCR, Western blot analyses, and mass spectrophotometry.

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Author information

Authors and Affiliations

  1. Dermatopathology Section, Department of Dermatology, Boston University School of Medicine, Boston, MA, USA

    Andrew Emley & Meera Mahalingam

  2. Boston University School of Medicine, Boston, MA, USA

    Kristen DeCarlo

  3. Dermatopathology Section, St John’s Institute of Dermatology, St. Thomas’ Hospital, London, UK

    Ophelia E. Dadzie

Authors
  1. Kristen DeCarlo
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  2. Andrew Emley
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  3. Ophelia E. Dadzie
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  4. Meera Mahalingam
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Corresponding author

Correspondence to Meera Mahalingam .

Editor information

Editors and Affiliations

  1. , Department of Pathology, University of Aberdeen, Foresterhill, Aberdeen, AB25 2ZD, United Kingdom

    Graeme I. Murray

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© 2011 Springer Science+Business Media, LLC

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DeCarlo, K., Emley, A., Dadzie, O.E., Mahalingam, M. (2011). Laser Capture Microdissection: Methods and Applications. In: Murray, G. (eds) Laser Capture Microdissection. Methods in Molecular Biology, vol 755. Humana Press. https://doi.org/10.1007/978-1-61779-163-5_1

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  • DOI: https://doi.org/10.1007/978-1-61779-163-5_1

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-162-8

  • Online ISBN: 978-1-61779-163-5

  • eBook Packages: Springer Protocols

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