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Laser Capture Microdissection

Methods and Protocols

  • Graeme I. Murray

Part of the Methods in Molecular Biology book series (MIMB, volume 755)

Table of contents

  1. Front Matter
    Pages i-xvii
  2. Kristen DeCarlo, Andrew Emley, Ophelia E. Dadzie, Meera Mahalingam
    Pages 1-15
  3. Lori A. Field, Brenda Deyarmin, Craig D. Shriver, Darrell L. Ellsworth, Rachel E. Ellsworth
    Pages 17-45
  4. Jian-Xin Lu, Cheuk-Chun Szeto
    Pages 47-56
  5. Michael A. Tangrea, Jeffrey C. Hanson, Robert F. Bonner, Thomas J. Pohida, Jaime Rodriguez-Canales, Michael R. Emmert-Buck
    Pages 57-66
  6. Danny Jonigk, Friedrich Modde, Clemens L. Bockmeyer, Jan Ulrich Becker, Ulrich Lehmann
    Pages 67-75
  7. Clemens L. Bockmeyer, Danny Jonigk, Hans Kreipe, Ulrich Lehmann
    Pages 85-94
  8. Donald J. Johann, Sumana Mukherjee, DaRue A. Prieto, Timothy D. Veenstra, Josip Blonder
    Pages 95-106
  9. Sarkawt M. Khoshnaw, Des G. Powe, Ian O. Ellis, Andrew R. Green
    Pages 119-142
  10. René B. H. Braakman, Theo M. Luider, John W. M. Martens, John A. Foekens, Arzu Umar
    Pages 143-154
  11. Isabelle Cadron, Toon Van Gorp, Philippe Moerman, Etienne Waelkens, Ignace Vergote
    Pages 155-163
  12. Patricia A. Meitner, Murray B. Resnick
    Pages 165-180
  13. Christopher C. Thorn, Deborah Williams, Thomas C. Freeman
    Pages 203-221
  14. Aamer M. Qazi, Sita Aggarwal, Christopher S. Steffer, David L. Bouwman, Donald W. Weaver, Scott A. Gruber et al.
    Pages 245-256
  15. Kerstin Stemmer, Daniel R. Dietrich
    Pages 279-290
  16. Anne Jørgensen, Marlene Danner Dalgaard, Si Brask Sonne
    Pages 307-313
  17. Charmaine Y. Pietersen, Maribel P. Lim, Laurel Macey, Tsung-Ung W. Woo, Kai C. Sonntag
    Pages 327-343
  18. Nivetha Murugesan, Jennifer Macdonald, Shujun Ge, Joel S. Pachter
    Pages 385-394
  19. Tomoatsu Kaneko, Takashi Okiji, Reika Kaneko, Hideaki Suda, Jacques E. Nör
    Pages 395-403
  20. Michael Beer, Sandra Doepping, Markus Hildner, Gabriele Weber, Rolf Grabner, Desheng Hu et al.
    Pages 417-428
  21. Yuditiya Purwosunu, Akihiko Sekizawa, Takashi Okai, Tetsuhiko Tachikawa
    Pages 477-489
  22. Back Matter
    Pages 491-493

About this book

Introduction

Laser microdissection techniques have revolutionized the ability of researchers in general, and pathologists in particular, to carry out molecular analysis on specific types of normal and diseased cells and to fully utilize the power of current molecular technologies including PCR, microarrays, and proteomics.  In second edition of Laser Capture Microdissection: Methods and Protocols, experts in the field provide the reader with practical advice on how to carry out tissue-based laser microdissection successfully in their own laboratory using the different laser microdissection systems that are available and to apply a wide range of molecular technologies.  The individual chapters encompass detailed descriptions of the individual laser based micro-dissection systems.  The downstream applications of the laser microdissected tissue described in the book include PCR in its many different forms as well as gene expression analysis including application to microarrays and proteomics. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, Laser Capture Microdissection: Methods and Protocols, Second Edition is an ideal resource for researchers striving to move forward our understanding of normal physiology and pathology.

Keywords

Downstream applications Gene expression analysis Microarrays Molecular analysis Molecular technologies PCR Proteomics Tissue-based laser microdissection

Editors and affiliations

  • Graeme I. Murray
    • 1
  1. 1., Department of PathologyUniversity of AberdeenAberdeenUnited Kingdom

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-61779-163-5
  • Copyright Information Springer Science+Business Media, LLC 2011
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-162-8
  • Online ISBN 978-1-61779-163-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site