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A Comparison of In Vitro and In Vivo Asexual Embryogenesis

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In Vitro Embryogenesis in Higher Plants

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1359))

Abstract

In plants, embryogenesis generally occurs through the sexual process of double fertilization, which involves a haploid sperm cell fusing with a haploid egg cell to ultimately give rise to a diploid embryo. Embryogenesis can also occur asexually in the absence of fertilization, both in vitro and in vivo. Somatic or gametic cells are able to differentiate into embryos in vitro following the application of plant growth regulators or stress treatments. Asexual embryogenesis also occurs naturally in some plant species in vivo, from either ovule cells as part of a process defined as apomixis, or from somatic leaf tissue in other species. In both in vitro and in vivo asexual embryogenesis, the embryo precursor cells must attain an embryogenic fate without the act of fertilization. This review compares the processes of in vitro and in vivo asexual embryogenesis including what is known regarding the genetic and epigenetic regulation of each process, and considers how the precursor cells are able to change fate and adopt an embryogenic pathway.

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Authors and Affiliations

  1. Commonwealth Scientific and Industrial Research Organization (CSIRO), Agriculture, Waite Campus, Urrbrae, South Australia

    Melanie L. Hand & Anna M. G. Koltunow

  2. Department of Biochemistry, University of Wageningen, Wageningen, 6703 HA, The Netherlands

    Sacco de Vries

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  1. Melanie L. Hand
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  2. Sacco de Vries
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  3. Anna M. G. Koltunow
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Correspondence to Anna M. G. Koltunow .

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  1. Dipartimento DEMETRA, Università degli Studi di Palermo, Palermo, Italy

    Maria Antonietta Germana

  2. National Research Council (CNR) IVALSA/Istituto per la Valorizzazione, Sesto Fiorentino, Florence, Italy

    Maurizio Lambardi

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Hand, M.L., de Vries, S., Koltunow, A.M.G. (2016). A Comparison of In Vitro and In Vivo Asexual Embryogenesis. In: Germana, M., Lambardi, M. (eds) In Vitro Embryogenesis in Higher Plants. Methods in Molecular Biology, vol 1359. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3061-6_1

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  • DOI: https://doi.org/10.1007/978-1-4939-3061-6_1

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3060-9

  • Online ISBN: 978-1-4939-3061-6

  • eBook Packages: Springer Protocols

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