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Applications of Yeast Surface Display for Protein Engineering

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Yeast Surface Display

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1319))

Abstract

The method of displaying recombinant proteins on the surface of Saccharomyces cerevisiae via genetic fusion to an abundant cell wall protein, a technology known as yeast surface display, or simply, yeast display, has become a valuable protein engineering tool for a broad spectrum of biotechnology and biomedical applications. This review focuses on the use of yeast display for engineering protein affinity, stability, and enzymatic activity. Strategies and examples for each protein engineering goal are discussed. Additional applications of yeast display are also briefly presented, including protein epitope mapping, identification of protein-protein interactions, and uses of displayed proteins in industry and medicine.

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Acknowledgements

Gerald M. Cherf is supported by the National Cancer Institute of the National Institutes of Health under Award Number F31CA186478, and funding from the Stanford Bioengineering Department. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.

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Authors and Affiliations

  1. Department of Bioengineering, Stanford University, Stanford, CA, 94305, USA

    Gerald M. Cherf & Jennifer R. Cochran

  2. Department of Chemical Engineering, Stanford University, Stanford, CA, 94305, USA

    Jennifer R. Cochran

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  1. Gerald M. Cherf
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  2. Jennifer R. Cochran
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Correspondence to Jennifer R. Cochran .

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  1. UCSF, San Francisco, California, USA

    Bin Liu

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Cherf, G.M., Cochran, J.R. (2015). Applications of Yeast Surface Display for Protein Engineering. In: Liu, B. (eds) Yeast Surface Display. Methods in Molecular Biology, vol 1319. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2748-7_8

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  • DOI: https://doi.org/10.1007/978-1-4939-2748-7_8

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2747-0

  • Online ISBN: 978-1-4939-2748-7

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