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Engineering of glycosylation in yeast and other fungi: current state and perspectives

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Abstract

With the increasing demand for recombinant proteins and glycoproteins, research on hosts for producing these proteins is focusing increasingly on more cost-effective expression systems. Yeasts and other fungi are promising alternatives because they provide easy and cheap systems that can perform eukaryotic post-translational modifications. Unfortunately, yeasts and other fungi modify their glycoproteins with heterogeneous high-mannose glycan structures, which is often detrimental to a therapeutic protein’s pharmacokinetic behavior and can reduce the efficiency of downstream processing. This problem can be solved by engineering the glycosylation pathways to produce homogeneous and, if so desired, human-like glycan structures. In this review, we provide an overview of the most significant recently reported approaches for engineering the glycosylation pathways in yeasts and fungi.

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Acknowledgments

The authors thank Dr. Amin Bredan for the help in preparing the manuscript. This research was supported by a Marie Curie Excellence Grant (MEXT-014292) and the Fund for Scientific Research-Flanders (FWO).

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  1. Unit for Molecular Glycobiology, Department for Molecular Biomedical Research, VIB-Ghent University, Technologiepark 927, 9052, Ghent-Zwijnaarde, Belgium

    Karen De Pourcq, Kristof De Schutter & Nico Callewaert

  2. Unit for Molecular Glycobiology, Department of Biomedical Molecular Biology, Ghent University, Technologiepark 927, 9052, Ghent, Belgium

    Karen De Pourcq & Kristof De Schutter

  3. Unit for Molecular Glycobiology, Department of Biochemistry and Microbiology, Ghent University, K.L.-Ledeganckstraat 35, 9000, Ghent, Belgium

    Nico Callewaert

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  1. Karen De Pourcq
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Correspondence to Nico Callewaert.

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De Pourcq, K., De Schutter, K. & Callewaert, N. Engineering of glycosylation in yeast and other fungi: current state and perspectives. Appl Microbiol Biotechnol 87, 1617–1631 (2010). https://doi.org/10.1007/s00253-010-2721-1

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  • DOI: https://doi.org/10.1007/s00253-010-2721-1

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