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Display of adenoregulin with a novel Pichia pastoris cell surface display system

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Abstract

Two Pichia pastoris cell surface display vectors were constructed. The vectors consisted of the flocculation functional domain of Flo 1p with its own secretion signal sequence or the α-factor secretion signal sequence, a polyhistidine (6×His) tag for detection, an enterokinase recognition site, and the insertion sites for target proteins. Adenoregulin (ADR) is a 33-amino-acid antimicrobial peptide isolated from Phyllomedusa bicolor skin. The ADR was expressed and displayed on the Pichia pastoris KM71 cell surface with the system reported. The displayed recombinant ADR fusion protein was detected by fluorescence microscopy and confocal laser scanning microscopy (CLSM). The antimicrobial activity of the recombinant adenoregulin was detected after proteolytic cleavage of the fusion protein on cell surface. The validity of the Pichia pastoris cell surface display vectors was proved by the displayed ADR.

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Authors and Affiliations

  1. State Key Laboratory of Bioreactor Engineering, Ne w Would Institute of Biotechnology, East China University of Science and Technology, 200237, Shanghai, P. R. China

    Ren Ren, Zhengbing Jiang, Meiyun Liu, Xinyi Tao, Yushu Ma & Dongzhi Wei

Authors
  1. Ren Ren
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  2. Zhengbing Jiang
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  3. Meiyun Liu
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  4. Xinyi Tao
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  5. Yushu Ma
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  6. Dongzhi Wei
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Corresponding authors

Correspondence to Yushu Ma or Dongzhi Wei.

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Ren, R., Jiang, Z., Liu, M. et al. Display of adenoregulin with a novel Pichia pastoris cell surface display system. Mol Biotechnol 35, 103–108 (2007). https://doi.org/10.1007/BF02686102

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  • DOI: https://doi.org/10.1007/BF02686102

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