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Coffee (Coffea arabica L.)

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Agrobacterium Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1224))

Abstract

Coffee (Coffea sp.) is a perennial plant widely cultivated in many tropical countries. It is a cash crop for millions of small farmers in these areas. As for other tree species, coffee has long breeding cycles, which makes conventional breeding programs time-consuming. For that matter, genetic transformation can be an effective way to introduce a desired trait in elite varieties or for functional genomics. In this chapter, we describe two highly efficient and reliable Agrobacterium-mediated transformation techniques developed for the C. arabica cultivated species: (1) A. tumefaciens to study and introduce genes conferring resistance/tolerance to biotic (coffee leaf rust, insects) and abiotic stress (drought, heat, seed desiccation) in fully transformed plants and (2) A. rhizogenes to study candidate gene expression for nematode resistance in transformed roots.

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Author information

Authors and Affiliations

  1. CIRAD, Centre de Coopération Internationale en Recherche Agronomique pour le Développement, UMR RPB, 911 Avenue Agropolis, BP 64501, 34398, Montpellier, France

    Eveline Déchamp & Jean-Christophe Breitler Ph.D.

  2. Centre de Coopération Internationale en Recherche Agronomique pour le Développement, UMR AGAP, 34398, Montpellier, France

    Thierry Leroy Ph.D.

  3. CIRAD, Centre de Coopération Internationale en Recherche Agronomique pour le Développement, UMR RPB, TA-A98/IRD, 911 Avenue Agropolis, BP 64501, 34398, Montpellier Cedex 5, France

    Hervé Etienne Ph.D.

Authors
  1. Eveline Déchamp
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  2. Jean-Christophe Breitler Ph.D.
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  3. Thierry Leroy Ph.D.
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  4. Hervé Etienne Ph.D.
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Corresponding author

Correspondence to Hervé Etienne Ph.D. .

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Editors and Affiliations

  1. Plant Sciences Institute, and Department of Agronomy, Center for Plant Transformation, Iowa State University, Ames, Iowa, USA

    Kan Wang

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Déchamp, E., Breitler, JC., Leroy, T., Etienne, H. (2015). Coffee (Coffea arabica L.). In: Wang, K. (eds) Agrobacterium Protocols. Methods in Molecular Biology, vol 1224. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-1658-0_22

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  • DOI: https://doi.org/10.1007/978-1-4939-1658-0_22

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  • Print ISBN: 978-1-4939-1657-3

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