Abstract
The production of ethanol and other biofuels through the biochemical conversion of lignocellulosic biomass represents a promising path towards sustainably achieving the immense global demand for liquid transportation fuels. While numerous cellulosic ethanol production process configurations exist, the one known as Consolidated Bioprocessing (CBP) stands alone in combining all biologically mediated events into the action of a single organism (i.e., production and secretion of saccharolytic enzymes, hydrolysis of cellulose and hemicellulose, and fermentation of six-carbon and five-carbon sugars into biofuels such as ethanol). We discuss here the major issues with developing CBP technologies including the promises and challenges, the two prominently pursued routes to achieve this technology and several of the most promising candidate organisms. CBP represents a low-risk, high-reward proposition and its pursuit by researchers is most certainly warranted as we look to the future.
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References
Lynd LR, Wyman CE, Gerngross TU (1999) Biocommodity engineering. Biotechnol Prog 15(5):777–793
Himmel ME (2007) Biomass recalcitrance: engineering plants and enzymes for biofuels production (vol 315, pg 804, 2007). Science 316(5827):982
Hill J, Nelson E, Tilman D et al (2006) Environmental, economic, and energetic costs and benefits of biodiesel and ethanol biofuels. Proc Natl Acad Sci USA 103(30):11206–11210
Lynd LR (1996) Overview and evaluation of fuel ethanol from cellulosic biomass: technology, economics, the environment, and policy. Annu Rev Energy Environ 21:403–465
Lynd LR, Weimer PJ, van Zyl WH et al (2002) Microbial cellulose utilization: fundamentals and biotechnology. Microbiol Mol Biol Rev 66(3):506–577
Den Haan R, Rose SH, Lynd LR et al (2007) Hydrolysis and fermentation of amorphous cellulose by recombinant Saccharomyces cerevisiae. Metab Eng 9(1):87–94
Lynd LR, van Zyl WH, McBride JE et al (2005) Consolidated bioprocessing of cellulosic biomass: an update. Curr Opin Biotechnol 16(5):577–583
Hong J, Wang Y, Kumagai H et al (2007) Construction of thermotolerant yeast expressing thermostable cellulase genes. J Biotechnol 130(2):114–123
van Zyl WH, Lynd LR, den Haan R et al (2007) Consolidated bioprocessing for bioethanol production using Saccharomyces cerevisiae. Adv Biochem Eng Biotechnol 108:205–235
Carere CR, Sparling R, Cicek N et al (2008) Third generation biofuels via direct cellulose fermentation. Int J Mol Sci 9(7):1342–1360
Lu YP, Zhang YHP, Lynd LR (2006) Enzyme-microbe synergy during cellulose hydrolysis by Clostridium thermocellum. Proc Natl Acad Sci USA 103(44):16165–16169
Xu Q, Singh A, Himmel ME (2009) Perspectives and new directions for the production of bioethanol using consolidated bioprocessing of lignocellulose. Curr Opin Biotechnol 20(3):364–371
Himmel ME, Ding SY, Johnson DK et al (2007) Biomass recalcitrance: engineering plants and enzymes for biofuels production. Science 315(5813):804–807
Foust TS, Ibsen KN, Dayton DC, Hess JR, Kenney KE (2008) The biorefinery, in biomass recalcitrance. In: Himmel ME (ed) Deconstructing the plant cell wall for bioenergy. Blackwell Publishing, London
McBee RH (1954) The characteristics of Clostridium thermocellum. J Bacteriol 67(4):505–506
Freier D, Mothershed CP, Wiegel J (1988) Characterization of Clostridium-thermocellum Jw20. Appl Environ Microbiol 54(1):204–211
Sai RM, Seenayya G (1989) Ethanol-production by Clostridium-thermocellum Ss8, a newly isolated thermophilic bacterium. Biotechnol Lett 11(8):589–592
Ram MS, Seenayya G (1991) Production of ethanol from straw and bamboo pulp by primary isolates of Clostridium thermocellum. World J Microbiol Biotechnol 7(3):372–378
Ram MS, Rao CV, Seenayya G (1991) Characteristics of Clostridium thermocellum strain Ss8—a broad saccharolytic thermophile. World J Microbiol Biotechnol 7(2):272–275
Herrero AA, Gomez RF, Snedecor B et al (1985) Growth-inhibition of Clostridium thermocellum by carboxylic-acids—a mechanism based on uncoupling by weak acids. Appl Microbiol Biotechnol 22(1):53–62
Tailliez P, Girard H, Longin R et al (1989) Cellulose fermentation by an asporogenous mutant and an ethanol-tolerant mutant of Clostridium thermocellum. Appl Environ Microbiol 55(1):203–206
Tailliez P, Girard H, Millet J et al (1989) Enhanced cellulose fermentation by an asporogenous and ethanol-tolerant mutant of Clostridium thermocellum. Appl Environ Microbiol 55(1):207–211
Rani KS, Seenayya G (1999) High ethanol tolerance of new isolates of Clostridium thermocellum strains SS21 and SS22. World J Microbiol Biotechnol 15(2):173–178
Pienkos PT, Zhang M (2009) Role of pretreatment and conditioning processes on toxicity of lignocellulosic biomass hydrolysates. Cellulose 16(4):743–762
Tyurin MV, Desai SG, Lynd LR (2004) Electrotransformation of Clostridium thermocellum. Appl Environ Microbiol 70(2):883–890
Tripathi SA, Olson DG, Argyros DA et al (2010) Development of pyrF-based genetic system for targeted gene deletion in Clostridium thermocellum and creation of a pta mutant. Appl Environ Microbiol 76(19):6591–6599
Zverlov VV, Klupp M, Krauss J et al (2008) Mutations in the scaffoldin gene, cipA, of Clostridium thermocellum with impaired cellulosome formation and cellulose hydrolysis: insertions of a new transposable element, IS1447, and implications for cellulase synergism on crystalline cellulose. J Bacteriol 190(12):4321–4327
Mai V, Lorenz WW, Wiegel J (1997) Transformation of Thermoanaerobacterium sp. strain JW/SL-YS485 with plasmid pIKM1 conferring kanamycin resistance. FEMS Microbiol Lett 148(2):163–167
Tyurin MV, Sullivan CR, Lynd LR (2005) Role of spontaneous current oscillations during high-efficiency electrotransformation of thermophilic anaerobes. Appl Environ Microbiol 71(12):8069–8076
Shaw AJ, Jenney FE, Adams MWW et al (2008) End-product pathways in the xylose fermenting bacterium, Thermoanaerobacterium saccharolyticum. Enzyme Microb Technol 42(6):453–458
Shaw AJ, Podkaminer KK, Desai SG et al (2008) Metabolic engineering of a thermophilic bacterium to produce ethanol at high yield. Proc Natl Acad Sci USA 105(37):13769–13774
Rabinovich ML, Melnik MS, Boloboba AV (2002) Microbial cellulases (review). Appl Biochem Microbiol 38(4):305–321
Harrison MJ, Nouwens AS, Jardine DR et al (1998) Modified glycosylation of cellobiohydrolase I from a high cellulase-producing mutant strain of Trichoderma reesei. Eur J Biochem 256(1):119–127
Srisodsuk M, Reinikainen T, Penttila M et al (1993) Role of the interdomain linker peptide of Trichoderma-reesei cellobiohydrolase-I in its interaction with crystalline cellulose. J Biol Chem 268(28):20756–20761
Knowles J, Lehtovaara P, Teeri T (1987) Cellulase families and their genes. Trends Biotechnol 5(9):255–261
Kumar R, Singh S, Singh OV (2008) Bioconversion of lignocellulosic biomass: biochemical and molecular perspectives. J Ind Microbiol Biotechnol 35(5):377–391
Ingram LO, Aldrich HC, Borges AC et al (1999) Enteric bacterial catalysts for fuel ethanol production. Biotechnol Prog 15(5):855–866
Tao H, Gonzalez R, Martinez A et al (2001) Engineering a homo-ethanol pathway in Escherichia coli: increased glycolytic flux and levels of expression of glycolytic genes during xylose fermentation. J Bacteriol 183(10):2979–2988
Zhou S, Davis FC, Ingram LO (2001) Gene integration and expression and extracellular secretion of Erwinia chrysanthemi endoglucanase CelY (celY) and CelZ (celZ) in ethanologenic Klebsiella oxytoca P2. Appl Environ Microbiol 67(1):6–14
Dien BS, Cotta MA, Jeffries TW (2003) Bacteria engineered for fuel ethanol production: current status. Appl Microbiol Biotechnol 63(3):258–266
Wood BE, Yomano LP, York SW et al (2005) Development of industrial-medium-required elimination of the 2,3-butanediol fermentation pathway to maintain ethanol yield in an ethanologenic strain of Klebsiella oxytoca. Biotechnol Prog 21(5):1366–1372
Yanase H, Nozaki K, Okamoto K (2005) Ethanol production from cellulosic materials by genetically engineered Zymomonas mobilis. Biotechnol Lett 27(4):259–263
Okamoto T, Yamano S, Ikeaga H et al (1994) Cloning of the Acetobacter xylinum cellulase gene and its expression in Escherichia coli and Zymomonas mobilis. Appl Microbiol Biotechnol 42(4):563–568
Brestic-Goachet N, Gunasekaran P, Cami B et al (1989) Transfer and expression of an Erwinia chrysanthemi cellulase gene in Zymomonas mobilis. J Gen Microbiol 135(4):893–902
Lejeune A, Eveleigh DE, Colson C (1988) Expression of an endoglucanase gene of Pseudomonas fluorescens var cellulosa in I. FEMS Microbiol Lett 49(3):363–366
Misawa N, Okamoto T, Nakamura K (1988) Expression of a cellulase gene in Zymomonas mobilis. J Biotechnol 7(3):167–178
Linger JG, Adney WS, Darzins A (2010) Heterologous expression and extracellular secretion of cellulolytic enzymes by Zymomonas mobilis. Appl Environ Microbiol 76(19):6360–6369
Penttila ME, Andre L, Lehtovaara P et al (1988) Efficient secretion of 2 fungal cellobiohydrolases by Saccharomyces cerevisiae. Gene 63(1):103–112
Van Rensburg P, Van Zyl WH, Pretorius IS (1998) Engineering yeast for efficient cellulose degradation. Yeast 14(1):67–76
van Rensburg P, van Zyl WH, Pretorius IS (1996) Co-expression of a Phanerochaete chrysosporium cellobiohydrolase gene and a Butyrivibrio fibrisolvens endo-beta-1,4-glucanase gene in Saccharomyces cerevisiae. Curr Genet 30(3):246–250
Agbogbo FK, Coward-Kelly G (2008) Cellulosic ethanol production using the naturally occurring xylose-fermenting yeast, Pichia stipitis. Biotechnol Lett 30(9):1515–1524
Prior BA, Kilian SG, Dupreez JC (1989) Fermentation of D-Xylose by the Yeasts Candida shehatae and Pichia stipitis—prospects and problems. Proc Biochem 24(1):21–32
Slininger PJ, Bolen PL, Kurtzman CP (1987) Pachysolen tannophilus—properties and process considerations for ethanol-production from D-xylose. Enzyme Microb Technol 9(1):5–15
Jeffries TW (2006) Engineering yeasts for xylose metabolism. Curr Opin Biotechnol 17(3):320–326
Agbogbo FK, Coward-Kelly G, Torry-Smith M et al (2006) Fermentation of glucose/xylose mixtures using Pichia stipitis. Proc Biochem 41(11):2333–2336
Delgenes JP, Moletta R, Navarro JM (1996) Effects of lignocellulose degradation products on ethanol fermentations of glucose and xylose by Saccharomyces cerevisiae, Zymomonas mobilis, Pichia stipitis, and Candida shehatae. Enzyme Microb Technol 19(3):220–225
Schneider H, Wang PY, Chan YK et al (1981) Conversion of D-xylose into ethanol by the yeast Pachysolen tannophilus. Biotechnol Lett 3(2):89–92
Wisselink HW, Toirkens MJ, Berriel MDF et al (2007) Engineering of Saccharomyces cerevisiae for efficient anaerobic alcoholic fermentation of L-arabinose. Appl Environ Microbiol 73(15):4881–4891
Becker J, Boles E (2003) A modified Saccharomyces cerevisiae strain that consumes L-arabinose and produces ethanol. Appl Environ Microbiol 69(7):4144–4150
Richard P, Verho R, Putkonen M et al (2003) Production of ethanol from L-arabinose by Saccharomyces cerevisiae containing a fungal L-arabinose pathway. FEMS Yeast Res 3(2):185–189
Karhumaa K, Wiedemann B, Hahn-Hagerdal B et al (2006) Co-utilization of L-arabinose and D-xylose by laboratory and industrial Saccharomyces cerevisiae strains. Microb Cell Fact 5:18
Ostergaard S, Olsson L, Nielsen J (2000) Metabolic engineering of Saccharomyces cerevisiae. Microbiol Mol Biol Rev 64(1):34–50
Vanarsdell JN, Kwok S, Schweickart VL et al (1987) Cloning, characterization, and expression in Saccharomyces cerevisiae of endoglucanase-I from Trichoderma-reesei. Biotechnology 5(1):60–64
Idiris A, Tohda H, Kumagai H et al (2010) Engineering of protein secretion in yeast: strategies and impact on protein production. Appl Microbiol Biotechnol 86(2):403–417
Karsch T, Stahl U, Esser K (1983) Ethanol production by Zymomonas and Saccharomyces, advantages and disadvantages. Eur J Appl Microbiol Biotechnol 18(6):387–391
van Rooyen R, Hahn-Hagerdal B, La Grange DC et al (2005) Construction of cellobiose-growing and fermenting Saccharomyces cerevisiae strains. J Biotechnol 120(3):284–295
McBride JE, Zietsman JJ, Van Zyl WH et al (2005) Utilization of cellobiose by recombinant beta-glucosidase-expressing strains of Saccharomyces cerevisiae: characterization and evaluation of the sufficiency of expression. Enzyme Microb Technol 37(1):93–101
Wood TM (1992) Fungal cellulases. Biochem Soc Trans 20(1):46–53
Den Haan R, Mcbride JE, La Grange DC et al (2007) Functional expression of cellobiohydrolases in Saccharomyces cerevisiae towards one-step conversion of cellulose to ethanol. Enzyme Microb Technol 40(5):1291–1299
Millis NF (1956) A study of the cider-sickness bacillus; a new variety of Zymomonas anaerobia. J Gen Microbiol 15(3):521–528
Rogers PL, Jeon YJ, Lee KJ et al (2007) Zymomonas mobilis for fuel ethanol and higher value products. Adv Biochem Eng Biotechnol 108:263–288
Lee KJ, Lefebvre M, Tribe DE et al (1980) High productivity ethanol fermentations with Zymomonas mobilis using continuous cell recycle. Biotechnol Lett 2(11):487–492
Lee KJ, Skotnicki ML, Tribe DE et al (1980) Kinetic-studies on a highly productive strain of Zymomonas mobilis. Biotechnol Lett 2(8):339–344
Rogers PL, Lee KJ, Tribe DE (1980) High productivity ethanol fermentations with Zymomonas mobilis. Proc Biochem 15(6):7–11
Lee KJ, Tribe DE, Rogers PL (1979) Ethanol-production by Zymomonas mobilis in continuous culture at high glucose concentrations. Biotechnol Lett 1(10):421–426
Rogers PL, Lee KJ, Tribe DE (1979) Kinetics of alcohol production by Zymomonas mobilis at high sugar concentrations. Biotechnol Lett 1(4):165–170
Skotnicki ML, Lee KJ, Tribe DE et al (1981) Comparison of ethanol-production by different Zymomonas strains. Appl Environ Microbiol 41(4):889–893
Swings J, Deley J (1977) Biology of Zymomonas. Bacteriol Rev 41(1):1–46
Spangler DJ, Emert GH (1986) Simultaneous saccharification fermentation with Zymomonas mobilis. Biotechnol Bioeng 28(1):115–118
Lee JH, Pagan RJ, Rogers PL (1983) Continuous simultaneous saccharification and fermentation of starch using Zymomonas mobilis. Biotechnol Bioeng 25(3):659–669
McMillan JD, Newman MM, Templeton DW et al (1999) Simultaneous saccharification and co-fermentation of dilute-acid pretreated yellow poplar hardwood to ethanol using xylose-fermenting Zymomonas mobilis. Appl Biochem Biotechnol 77–9:649–665
Metabolic Engineering of a Pentose Metabolism Pathway in Ethanologenic Zymomonas mobilis Min Zhang, Christina Eddy, Kristine Deanda, Mark Finkelstein, and Stephen Picataggio Science 13 January 1995: 267 (5195), 240-243. [DOI:10.1126/science.267.5195.240]
Deanda K, Zhang M, Eddy C et al (1996) Development of an arabinose-fermenting Zymomonas mobilis strain by metabolic pathway engineering. Appl Environ Microbiol 62(12):4465–4470
Carey VC, Walia SK, Ingram LO (1983) Expression of a lactose transposon (Tn951) in Zymomonas mobilis. Appl Environ Microbiol 46(5):1163–1168
Yanase H, Kurii J, Tonomura K (1986) Construction of a promoter-cloning vector in Zymomonas mobilis. Agric Biol Chem 50(11):2959–2961
Byun MOK, Kaper JB, Ingram LO (1986) Construction of a new vector for the expression of foreign genes in Zymomonas mobilis. J Ind Microbiol 1(1):9–15
Yoon P (1988) Pack, transfer of bacillus subtilis endo-β-1,4-glucanase gene into Zymomonas anaerobia. Biotechnol Lett 10(3):213–216
Sandkvist M (2001) Biology of type II secretion. Mol Microbiol 40(2):271–283
Lee PA, Tullman-Ercek D, Georgiou G (2006) The bacterial twin-arginine translocation pathway. Annu Rev Microbiol 60:373–395
Mergulhao FJ, Summers DK, Monteiro GA (2005) Recombinant protein secretion in Escherichia coli. Biotechnol Adv 23(3):177–202
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Linger, J.G., Darzins, A. (2013). Consolidated Bioprocessing. In: Lee, J. (eds) Advanced Biofuels and Bioproducts. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-3348-4_16
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