Summary
The endo-β-1,4-glucanase gene ofBacillus subtilis origin cloned previously in a plasmid pBS1 was subcloned in a new plasmid pSCR815, and with the new plasmidZymomonas anaerobia was transformed. TheBacillus glucanase gene expressed in theZymomonas cells with efficiency much lower than inEscherichia coli.
Similar content being viewed by others
References
Birnboim, H. C. and Doly, J. (1979). Nucleic Acid Res. 7, 1515–1523.
Browne, G. M., Skotnicki, M. L., Goodman, A. E. and Rogers, P. L. (1984). Plasmid. 12, 211–214.
Goodman, H. M. and Macdonald, R. J. (1979). In: Method in Enzymology, R., Wu, eds. vol. 68, pp. 75–90, New York: Academic Press.
Park, S. H. and Pack, M. Y. (1986). Enzyme Micro. Technol. 8, 725–728.
Teather, R. M. and Wood, P. J. (1982). Appl. Environ. Microbiol. 43, 777–780.
Yoon, K. H. and Pack, M. Y. (1987). Biotechnol. Letter. 9, 163–168.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Yoon, K.H., Park, S.H. & Pack, M.Y. Transfer ofBacillus subtilis endo-β-1,4-glucanase gene intoZymomonas anaerobia . Biotechnol Lett 10, 213–216 (1988). https://doi.org/10.1007/BF01134832
Issue Date:
DOI: https://doi.org/10.1007/BF01134832