Summary
Ethanol production byZ.mobilis has been studied in continuous culture with 10, 15 and 20% glucose media. At 10% glucose, steady state conditions were achieved under glucose-limited conditions. At 15 and 20% glucose, the glucose was not fully metabolized even at low dilution rates and oscillatory behavior was evident. It is proposed that ethanol inhibition of growth is responsible for these phenomena. Comparison of kinetic parameters with those from previously published batch data revealed similar values. The maintenance energy coefficient (m) forZ.mobilis was relatively high and was calculated as 1.6 g/g/h for 10% glucose and 3.1 g/g/h for 15% glucose.
Similar content being viewed by others
References
Dean, A.C.R. and Rogers, P.L. (1967)Biochim. Biophys. Acta, 148, 280–292.
Gray, P.P. and Rogers, P.L. (1971a)Biochim. Biophys. Acta, 230, 393–400.
Gray, P.P. and Rogers, P.L. (1971b)Biochim. Biophys. Acta, 230, 401–410.
Hempfling, W.P. and Mainzer, S.E. (1975)J.Bacteriol. 123, 1076–1087.
Pirt, S.J. (1975) Principles of Microbe and Cell Cultivation. Blackwell Scientific Publications, p.67.
Rogers, P.L., Kye Joon Lee and Tribe, D.E. (1979)Biotechnology Letters, 1, 165–170.
Sawada, H. and Rogers, P.L. (1977).J.Ferm.Technol. 55, 297–310.
Swings, J. and De Ley, J. (1977)Bacteriol. Reviews, 41, 1–46.
Stouthamer, A.H. and Bettenhaussen, C. (1973)Biochim. Biophys. Acta, 301, 53–70.
Stouthamer, A.H. (1977)Symp.Soc.Gen.Microbiol. 27, 285–315.
Tempest, D.W. (1978)Trends in Biochem. Sciences, 3, 180–185.
Watson, T.G. (1970)J.Gen.Microbiol. 64, 91–99.
Zines, D.O. and Rogers, P.L. (1970)Biotech. Bioeng. 12, 561–575.
Zines, D.O. and Rogers, P.L. (1971)Biotech. Bioeng. 13, 293–308.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Joon Lee, K., Tribe, D.E. & Rogers, P.L. Ethanol production byZymomonas mobilis in continuous culture at high glucose concentrations . Biotechnol Lett 1, 421–426 (1979). https://doi.org/10.1007/BF01388079
Issue Date:
DOI: https://doi.org/10.1007/BF01388079