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Aerobic Utilization of Methanol for Microbial Growth and Production

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One-Carbon Feedstocks for Sustainable Bioproduction

Part of the book series: Advances in Biochemical Engineering/Biotechnology ((ABE,volume 180))

Abstract

Methanol is a reduced one-carbon (C1) compound. It supports growth of aerobic methylotrophs that gain ATP from reduced redox equivalents by respiratory phosphorylation in their electron transport chains. Notably, linear oxidation of methanol to carbon dioxide may yield three reduced redox equivalents if methanol oxidation is NAD-dependent as, e.g., in Bacillus methanolicus. Methanol has a higher degree of reduction per carbon than glucose (6 vs. 4), and thus, lends itself as an ideal carbon source for microbial production of reduced target compounds. However, C–C bond formation in the RuMP or serine cycle, a prerequisite for production of larger molecules, requires ATP and/or reduced redox equivalents. Moreover, heat dissipation and a high demand for oxygen during catabolic oxidation of methanol may pose challenges for fermentation processes. In this chapter, we summarize metabolic pathways for aerobic methanol utilization, aerobic methylotrophs as industrial production hosts, strain engineering, and methanol bioreactor processes. In addition, we provide technological and market outlooks.

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Authors and Affiliations

  1. Genetics of Prokaryotes, Faculty of Biology and CeBiTec, Bielefeld University, Bielefeld, Germany

    Volker F. Wendisch

  2. Acies Bio d.o.o., Ljubljana, Slovenia

    Gregor Kosec

  3. LISBP, Université de Toulouse, CNRS, INRA, INSA, Toulouse, France

    Stéphanie Heux

  4. Department of Biotechnology and Food Sciences, Norwegian University of Science and Technology (NTNU), Trondheim, Norway

    Trygve Brautaset

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  1. Volker F. Wendisch
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  4. Trygve Brautaset
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  1. Institute for Bioprocess and Biosystems Engineering, Hamburg University of Technology, Hamburg, Germany

    An-Ping Zeng

  2. Laboratory of Microbiology, Wageningen University, WAGENINGEN, The Netherlands

    Nico J. Claassens

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Wendisch, V.F., Kosec, G., Heux, S., Brautaset, T. (2021). Aerobic Utilization of Methanol for Microbial Growth and Production. In: Zeng, AP., Claassens, N.J. (eds) One-Carbon Feedstocks for Sustainable Bioproduction. Advances in Biochemical Engineering/Biotechnology, vol 180. Springer, Cham. https://doi.org/10.1007/10_2021_177

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