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Alleviation of cold stress in wheat with psychrotrophic phosphorus solubilizing Acinetobacter rhizosphaerae EU-KL44

  • Soil and Agricultural Microbiology - Research Paper
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Abstract

Low-temperature stress can seriously impair plant physiology. Chilling injury leads to a complex array of cellular dysfunctions, and symptoms include chlorosis, sterility, loss of vigor, wilting, and even death of the plants. Furthermore, phosphorus limitations additionally halt the growth of plants. Low-temperature adaptive plant growth–promoting microbes through various direct and indirect mechanisms help in the survival of plants under stress conditions. The present investigation deals with isolation of P-solubilizing psychrotrophic bacteria from diverse cultivars of wheat grown in the Keylong region of Himachal Pradesh. A total of 33 P-solubilizing bacterial isolates were obtained. P-solubilizers were screened for different plant growth–promoting (PGP) attributes of K and Zn solubilization, production of IAA, siderophores, and different hydrolytic enzymes. Among 33 P-solubilizers, 8 efficient strains exhibiting multiple PGP attributes were used as bioinoculants for wheat under low-temperature stress in different in vitro and in vivo experiments. The psychrotrophic bacterial isolates positively influenced the growth and physiological parameters as well as nutrient uptake and yield of wheat and efficiently alleviated low-temperature stress. The potential of low-temperature stress adaptive and PGP microbes can be utilized in agricultural sector for amelioration of low-temperature stress and plant growth promotion. The present study deals with the isolation of psychrotrophic P-solubilizers with multiple PGP attributes and their role in alleviation of cold stress in wheat.

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Acknowledgements

The authors are grateful to Department of Biotechnology, Dr. Khem Singh Gill Akal College of Agriculture, Eternal University, Baru Sahib and the Department of Environment, Science & Technology (DEST), Shimla-funded project “Development of Microbial Consortium as Bio-inoculants for Drought and Low Temperature Growing Crops for Organic Farming in Himachal Pradesh” for providing the facilities, to undertake the investigations.

Funding

The Department of Environment, Science & Technology (DEST), Shimla-funded project “Development of Microbial Consortium as Bio-inoculants for Drought and Low Temperature Growing Crops for Organic Farming in Himachal Pradesh” provided financial support, to undertake the investigations.

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  1. Department of Microbiology, Akal College of Basic Sciences, Eternal University, Baru Sahib, Sirmour, 173101, India

    Divjot Kour

  2. Department of Biotechnology, Dr. Khem Singh Gill Akal College of Agriculture, Eternal University, Baru Sahib, Sirmour, 173101, India

    Ajar Nath Yadav

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Kour, D., Yadav, A.N. Alleviation of cold stress in wheat with psychrotrophic phosphorus solubilizing Acinetobacter rhizosphaerae EU-KL44. Braz J Microbiol 54, 371–383 (2023). https://doi.org/10.1007/s42770-023-00913-7

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