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Alleviation of cold stress in inoculated wheat (Triticum aestivum L.) seedlings with psychrotolerant Pseudomonads from NW Himalayas

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Abstract

Twelve psychrotolerant Pseudomonad strains were selected on the basis of various plant growth-promoting (PGP) activities at cold temperature (4°C). The effect of inoculation with Pseudomonad strains on cold alleviation and growth of wheat seedling at cold temperature (8°C) was investigated under greenhouse condition. Inoculation with Pseudomonad strains significantly enhanced root/shoot biomass and nutrients uptake as compared to non-bacterized control at 60 days of plant growth. Bacterization significantly improved the level of cellular metabolites like chlorophyll, anthocyanin, free proline, total phenolics, starch content, physiologically available iron, proteins, and amino acids that are sign of alleviation of cold stress in wheat plants. Increased relative water content, reduced membrane injury (electrolyte leakage), and Na+/K+ ratio were also recorded in bacterized wheat plants. Electrolyte leakage and Na+/K+ were found inversely proportional to plant growth at cold temperature. Statistical analysis of twenty-three measured parameters revealed that uninoculated control was under cold stress while eight bacterial strains were positively alleviating cold stress in wheat plants. Thus, the psychrotrophic Pseudomonad strains could effectively provide a promising solution to overcome cold stress, which is major factor hindering wheat productivity under cold climatic condition.

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Acknowledgments

This research work was supported by Indian Council of Agricultural Research through the project “Application of Microorganisms in Agriculture and Allied Sectors (AMAAS)”, and our special thanks to Mr. Sanjay Kumar for his technical assistance during plant nutrient and soil analysis and Mr. K. K. S. Bisht, In-charge, Social Science Section, VPKAS, Almora, India, for the valuable suggestion during statistical analysis.

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  1. Vivekananda Institute of Hill Agriculture, Indian Council of Agricultural Research, Almora, 263601, Uttarakhand, India

    Pankaj Kumar Mishra, Shekhar Chandra Bisht, Pooja Ruwari, Jaideep Kumar Bisht & Jagdish Chandra Bhatt

  2. Division of Soil Sciences, Indian Institute of Horticultural Research, Bangalore, India

    Govindan Selvakumar

  3. Department of Biotechnology, HNB Garhwal University, Srinagar, 246174, Uttarakhand, India

    Gopal Krishna Joshi

  4. Indian Agricultural Research Institute, (I.C.A.R.), Pusa, New Delhi, India

    Hari Shankar Gupta

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Mishra, P.K., Bisht, S.C., Ruwari, P. et al. Alleviation of cold stress in inoculated wheat (Triticum aestivum L.) seedlings with psychrotolerant Pseudomonads from NW Himalayas. Arch Microbiol 193, 497–513 (2011). https://doi.org/10.1007/s00203-011-0693-x

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