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A Bacillaceae consortium positively impacts arbuscular mycorrhizal fungus colonisation, plant phosphate nutrition, and tuber yield in Solanum tuberosum cv. Jazzy

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Abstract

In soils, phosphorus (P) is present in relatively large amounts, but for plants and microorganisms, P remains complexed under unavailable forms. To access the unavailable forms of P, plants interact with soil microorganisms such as arbuscular mycorrhizal fungi (AMF) and phosphate degrading bacteria (PDB) that can act synergically to improve plant P nutrition. In practice, we aim to stimulate microorganism properties for phosphate degradation and transport to the plant in agricultural soils.

Firstly, 17 bacterial isolates were characterized for their ability to degrade insoluble P complexes and plant growth promoting rhizobacteria (PGPR) traits such as the production of auxins and the formation of biofilms via the production of extracellular polymeric substances. Secondly, two Bacillaceae isolates were selected regarding their compatibility in co-culture and ability to promote PGPR traits. In the greenhouse and in pots containing an agricultural soil, tubers of Solanum tuberosum cv. Jazzy were inoculated with each individual isolate, and both isolates. We showed that bacterial inoculation positively impacted plant P nutrition, growth, and yield as well as indigenous arbuscular mycorrhizal rate. Our results suggest that the bacterial consortium synergically interacts with indigenous AMF community to improve plant P nutrition and yield, without changing associated microbial communities.

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Acknowledgements

This research was jointly supported by the CIFRE program (CIFRE industrial research agreements n°2019/0129) between Premier Tech Producteurs et Consommateurs, France and the General Direction for Higher Education of the Ministry for Education and Research of France. The help of Célien Durney, Louise Védrenne, Valérie Monfort-Pimet, Noureddine El-Mjiyad, Felix LaRoche-Johnston, Chantal Brosseau, and Geneviève Lachance in performing the experiments was greatly acknowledged.

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Authors and Affiliations

  1. Agroécologie, Institut Agro Dijon, CNRS, Université de Bourgogne, INRAE, Université de Bourgogne Franche-Comté, Dijon, France

    Raphael Boussageon, Antoine Sportes, Jean-Paul Lemaitre, Diederick van Tuinen, Daniel Wipf & Pierre-Emmanuel Courty

  2. Premier Tech Producteurs et Consommateurs, Maine et Loire, 28 Rue Des Epinettes, 49680, Vivy, France

    Raphael Boussageon & Carmen Lapadatescu

  3. Premier Tech Growers & Consumers, 1 avenue Premier, Campus Premier Tech, G5R 6C1, QC, Rivière-du-Loup, Canada

    Martin Trépanier

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  1. Raphael Boussageon
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  2. Antoine Sportes
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  4. Diederick van Tuinen
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  8. Pierre-Emmanuel Courty
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Contributions

R.B., A.S performed the experiments. R.B., C.L., M.T., P-E.C., D.W. conceived and designed the experiments. R.B., J.L., C.L., M.T., P-E.C., D.W. wrote and revised the paper.

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Correspondence to Pierre-Emmanuel Courty.

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Boussageon, R., Sportes, A., Lemaitre, JP. et al. A Bacillaceae consortium positively impacts arbuscular mycorrhizal fungus colonisation, plant phosphate nutrition, and tuber yield in Solanum tuberosum cv. Jazzy. Symbiosis 89, 235–250 (2023). https://doi.org/10.1007/s13199-023-00904-0

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