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Transgenic tetraploid Isatis indigotica expressing Bt Cry1Ac and Pinellia ternata agglutinin showed enhanced resistance to moths and aphids

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Abstract

Co-expression of multiple genes encoding different kinds of insect resistant proteins has been developed to confer a broader spectrum of pest control. Tetraploid Isatis indigotica Fort was transformed with a plasmid, p3300BP, containing Bacillus thuringiensis Cry1Ac gene (Bt) and Pinellia ternata agglutinin gene (Pta) and the selectable marker herbicide resistance gene (Bar) driven by the CaMV35S promoter via Agrobacterium tumefaciens-mediated transformation. The integration and expression of introduced genes in regenerated transgenic plants were confirmed by PCR and Western blot assays. Insect bioassay test demonstrated transgenic lines had significant inhibition to diamondback moths (Plutella xylostella L.) and peach potato aphids (Myzus persicae Sulzer) simultaneously. Our study reported here would be a great motivation for field culture of tetraploid I. indigotica, also providing an efficient molecular breeding strategy to provide insect tolerant plants.

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Acknowledgments

This research was financially supported by National Natural Science Foundation of China (30900786); Modernization of Traditional Chinese Medicine Foundation (08DZ1971502) and Domestic Science and Technology Cooperation Projects (10495801400, 10395820200), Shanghai Science and Technology Committee.

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Authors and Affiliations

  1. Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, Shanghai, 200433, China

    Ying Xiao, Kai Wang, Ruxian Ding, Hanming Zhang & Lei Zhang

  2. Department of Pharmacy, Changzheng Hospital, Second Military Medical University, Shanghai, 200003, China

    Ying Xiao, Peng Di, Junfeng Chen & Wansheng Chen

  3. Modern Research Center for Traditional Chinese Medicine, Second Military Medical University, Shanghai, 200433, China

    Junfeng Chen & Wansheng Chen

Authors
  1. Ying Xiao
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  2. Kai Wang
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  3. Ruxian Ding
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  4. Hanming Zhang
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  5. Peng Di
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  6. Junfeng Chen
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  7. Lei Zhang
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  8. Wansheng Chen
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Correspondence to Lei Zhang or Wansheng Chen.

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Xiao, Y., Wang, K., Ding, R. et al. Transgenic tetraploid Isatis indigotica expressing Bt Cry1Ac and Pinellia ternata agglutinin showed enhanced resistance to moths and aphids. Mol Biol Rep 39, 485–491 (2012). https://doi.org/10.1007/s11033-011-0762-x

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  • DOI: https://doi.org/10.1007/s11033-011-0762-x

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