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Developing new markers and QTL mapping for greenbug resistance in sorghum [Sorghum bicolor (L.) Moench]

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Abstract

Greenbug is a major damaging insect to sorghum production in the United States. Among various virulent greenbug biotypes, biotype I is the most predominant and severe for sorghum. To combat with the damaging pest, greenbug resistant sources were obtained from screening sorghum germplasm collection. This experiment was conducted to identify the genomic regions contributing resistance to greenbug biotype I in a sorghum accession, PI 607900. An F2 mapping population consisting of 371 individuals developed from a cross of the resistant line with an elite cultivar, BTx623 (susceptible) were tested and scored for their response to greenbug feeding in the greenhouse. Significant differences in resistance were observed between the two parental lines and among their F2 progeny in response to greenbug feeding at 7, 10, 14 and 21 days after infestation. A linkage map spanning a total length of 729.5 cM across the genome was constructed with 102 polymorphic SSR markers (69 genomic and 33 EST SSRs). Of those microsatellite markers, 48 were newly developed during this study, which are a useful addition for sorghum genotyping and genome mapping. Single marker analysis revealed 29 markers to be significantly associated with the plant response to greenbug feeding damage. The results from interval mapping, composite interval mapping and multiple interval mapping analyses identified four major QTLs for greenbug resistance on chromosome 9. These QTLs collectively accounted for 34–82 % of the phenotypic variance in greenbug resistance. Minor QTLs located on chromosome 3 explained 1 % of the phenotypic variance in greenbug resistance. The major allele for greenbug resistance was on chromosome 9 close to receptor-like kinase Xa21-binding protein 3. These markers are useful to screen more resistant genotypes. Furthermore, the markers tagged to QTL regions can be used to enhance the sorghum breeding program for greenbug resistance through marker-assisted selection and map-based cloning.

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Acknowledgments

The author would like to thank Dr. Kathleen Yeaters, Area Statistician, ARS, College Station, TX for her assistance in statistical analysis. The author would also thank Ms. Angela Phillips for technical support during the project. The author would like to acknowledge the funding support received from Oklahoma Sorghum Commission for the project.

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Authors and Affiliations

  1. Department of Botany, Oklahoma State University, Stillwater, OK, 74078, USA

    Somashekhar Punnuri, Yinghua Huang & Janette Steets

  2. USDA-ARS Plant Science Research Laboratory, 1301 N. Western Road, Stillwater, OK, 74075, USA

    Yinghua Huang

  3. Department of Plant and Soil Sciences, Oklahoma State University, Stillwater, OK, 74078, USA

    Yanqi Wu

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  1. Somashekhar Punnuri
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Correspondence to Yinghua Huang.

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Punnuri, S., Huang, Y., Steets, J. et al. Developing new markers and QTL mapping for greenbug resistance in sorghum [Sorghum bicolor (L.) Moench]. Euphytica 191, 191–203 (2013). https://doi.org/10.1007/s10681-012-0755-4

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