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Molecular mapping of QTLs for resistance to the greenbug Schizaphis graminum (Rondani) in Sorghum bicolor (Moench)

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Abstract

Sorghum is a worldwide important cereal crop and widely cultivated for grain and forage production. Greenbug, Schizaphis graminum (Rondani) is one of the major insect pests of sorghum and can cause serious damage to sorghum plants, particularly in the US Great Plains. Identification of chromosomal regions responsible for greenbug resistance will facilitate both map-based cloning and marker-assisted breeding. Thus, a mapping experiment was conducted to dissect sorghum genetic resistance to greenbug biotype I into genomic regions. Two hundred and seventy-seven (277) F2 progeny and their F2:3 families from a cross between Westland A line (susceptible parent) and PI550610 (resistant parent) combined with 118 polymorphic simple sequence repeat (SSR) markers were used to map the greenbug resistance QTLs. Composite interval mapping (CIM) and multiple interval mapping (MIM) revealed two QTLs on sorghum chromosome nine (SBI-09) consistently conditioned the resistance of host plant to the greenbug. The two QTLs were designated as QSsgr-09-01 (major QTL) and QSsgr-09-02 (minor QTL), accounting for approximately 55–80%, and 1–6% of the phenotypic variation for the resistance to greenbug feeding, respectively. These resistance QTLs appeared to have additive and partially dominant effects. The markers Xtxp358, Xtxp289, Xtxp67 and Xtxp230 closely flanked the respective QTLs, and can be used in high-throughput marker-assisted selections (MAS) for breeding new resistant parents and producing commercial hybrids.

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Acknowledgments

Authors would like to acknowledge Ms. Angela Phillips and Miss Lindsey Hollaway for their excellent technical assistance. Special thanks are due to Dr. S. C. Wang of Bioinformatics Research Center at North Carolina State University for his constructive suggestions in the use of Windows QTL cartographer, and to Dr. J. Burd for providing original greenbug cultures.

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  1. Yanqi Wu

    Present address: Department of Plant and Soil Sciences, Oklahoma State University, Stillwater, OK, 74078, USA

Authors and Affiliations

  1. USDA-ARS Plant Science Research Laboratory, 1301 N. Western Rd, Stillwater, OK, 74075, USA

    Yanqi Wu & Yinghua Huang

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  1. Yanqi Wu
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  2. Yinghua Huang
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Correspondence to Yinghua Huang.

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Communicated by M. Bohn.

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Wu, Y., Huang, Y. Molecular mapping of QTLs for resistance to the greenbug Schizaphis graminum (Rondani) in Sorghum bicolor (Moench). Theor Appl Genet 117, 117–124 (2008). https://doi.org/10.1007/s00122-008-0757-8

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  • DOI: https://doi.org/10.1007/s00122-008-0757-8

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