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Characterization and mapping of simple sequence repeats (SSRs) in Sorghum bicolor

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Abstract

 A total of 13 SSR loci were characterized in Sorghum bicolor. Ten of these loci were isolated by screening sorghum genomic AG-enriched libraries with labelled poly(AG)/poly(CT), the other three were derived from database searches. In order to explore the degree of polymorphism detectable in this species by this type of molecular marker, the SSR markers were tested on nine inbred lines of S. bicolor of different geographic origin. PCR analysis on acrylamide gels revealed a high degree of polymorphism (δT=0.80). One locus, in particular, allowed the identification of all of the nine inbred lines used in our study. Seven of these SSR markers were mapped, using an existing sorghum RFLP map.

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Authors and Affiliations

  1. Dipartimento di Genetica e di Biologia dei Microrganismi, Universita’ degli studi di Milano, Via Celoria 26, 20133 Milano, Italy, , , , , , IT

    G. Taramino, R. Tarchini, S. Ferrario & M. E. Pe’

  2. Department of Agronomy, Iowa State University, Ames, IA 50011 USA, , , , , , US

    M. Lee

Authors
  1. G. Taramino
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  2. R. Tarchini
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  3. S. Ferrario
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  4. M. Lee
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  5. M. E. Pe’
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Received: 13 June 1996 / Accepted: 27 September 1996

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Taramino, G., Tarchini, R., Ferrario, S. et al. Characterization and mapping of simple sequence repeats (SSRs) in Sorghum bicolor. Theor Appl Genet 95, 66–72 (1997). https://doi.org/10.1007/s001220050533

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  • DOI: https://doi.org/10.1007/s001220050533

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