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Development of SCAR and CAPS markers linked to a recessive male sterility gene in lettuce (Lactuca sativa L.)

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Abstract

Genetic male sterility (GMS) has been a useful system for the production of hybrid varieties in self-pollinated plants. We obtained a GMS line developed from a spontaneous mutation in lettuce (Lactuca sativa L.). Genetic analysis in our previous study revealed that the sterility was controlled by a recessive gene which was named ms-S. For simple and quick screening of individuals showing male sterility, we attempted molecular mapping of the ms-S locus using an amplified fragment length polymorphism (AFLP) technique. From the examination of 4,096 AFLP primer combinations, 63 AFLP markers were found to be linked to the gene and nine of them were successfully converted into sequence characterized amplified region (SCAR) markers and cleaved amplified polymorphic sequence (CAPS) markers. Linkage analysis indicated that these nine markers were closely linked to the ms-S gene and all were located on the same side of the gene. The minimum genetic distance between the ms-S gene and a marker was 3.1 cM. These results provide additional information for map-based cloning of the ms-S gene and will be of great help for lettuce breeding using GMS to produce F1 hybrids.

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References

  • Chen J, Hu J, Vick BA, Jan CC (2006) Molecular mapping of a nuclear male-sterility gene in sunflower (Helianthus annuus L.) using TRAP and SSR markers. Theor Appl Genet 113:122–127

    Article  PubMed  CAS  Google Scholar 

  • Dogget H (1972) Recurrent selection in Sorghum populations. Heredity 28:9–29

    Article  Google Scholar 

  • Dovora L, Frankel R (1961) Practical aspects and the use of male sterility in the production of hybrid tomato seed. Euphytica 16:300–310

    Google Scholar 

  • Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small quantities of flesh leaf tissue. Phytochem Bull 19:11–15

    Google Scholar 

  • Farrara B, Hott TW, Michelmore RW (1987) Genetic analysis of factors for resistance to downy mildew (Bremia lactucae) in lettuce (Lactuca sativa). Plant Pathol 36:499–514

    Article  Google Scholar 

  • Goubara M, Takasaki T (2004) Pollination effect of the sweet bee Lasioglossum villosulum trichopse (Hymenoptera: Halictidae) on genic male-sterile lettuce. Appl Entomol Zool 39:163–169

    Article  Google Scholar 

  • Hayashi M, Miyahara A, Sato S, Kato T, Yoshikawa M, Taketa M, Hayashi M, Pedrosa A, Onda R, Imaizumi-Anraku H, Bachmair A, Sandal N, Stougaard J, Murooka Y, Tabata S, Kawasaki S, Kawaguchi M, Harada K (2001) Construction of a genetic linkage map of the model legume Lotus japonicus using an intraspecific F2 population. DNA Res 8:301–310

    Article  PubMed  CAS  Google Scholar 

  • Hockett EA, Eslick RF (1970) Genetic male sterile genes useful in hybrid barley production. In: Nilan RA (ed) Barley genetics II. Washington State University Press, Pullman, pp 298–307

    Google Scholar 

  • Hubbard JC, Gerikj JS (1993) New wilt disease of lettuce incited by Fusarium oxysporum f. sp. lactucum forma specialis nov. Plant Dis 77:750–755

    Article  Google Scholar 

  • Konieczny A, Ausubel F (1993) A procedure for mapping Arabidopsis mutations using co-dominant ecotype-specific PCR based markers. Plant J 4:403–410

    Article  PubMed  CAS  Google Scholar 

  • Kosambi DD (1944) The estimation of map distances from recombination value. Ann Eugene 12:172–175

    Article  Google Scholar 

  • Kuwata S, Kubo S, Yamashita S, Doi Y (1983) Rod-shaped particles, a probable entity of lettuce big vein virus. Ann Phytopathol Soc Jpn 49:246–251

    Google Scholar 

  • Langton FA, Smith JWM, Edmondson RN (1990) Heterosis in crisphead lettuce (Lactuca sativa L.) hybrids. Euphytica 49:15–23

    Article  Google Scholar 

  • Michelmore RW (2009) California leafy greens research program. http://calgreens.org/control/uploads/Genetic_Variation_in_Lettuce_-_Michelmore.pdf

  • Michelmore RW, Paran I, Kesseli RV (1991) Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci USA 88:9828–9832

    Article  PubMed  CAS  Google Scholar 

  • Mienie CMS, Liebenberg MM, Pretorius ZA, Miklas PN (2005) SCAR markers linked to the common bean rust resistance gene Ur-13. Theor Appl Genet 111:972–979

    Article  PubMed  CAS  Google Scholar 

  • Neff MM, Neff JD, Chory J, Pepper AE (1998) dCAPS, a simple technique for the genetic analysis of single nucleotide polymorphisms: experimental applications in Arabidopsis thaliana genetics. Plant J 14:387–392

    Article  PubMed  CAS  Google Scholar 

  • Paran I, Michelmore RW (1993) Development of reliable PCR-based markers linked to downy mildew resistance genes in lettuce. Theor Appl Genet 85:985–993

    Article  CAS  Google Scholar 

  • Robinson RW, McCreight JD, Ryder EJ (1983) The genes of lettuce and closely related species. In: Janick J (ed) Plant breeding reviews, vol 1. Avi, Westport, pp 267–293

    Google Scholar 

  • Rozen S, Skaletsky H (2000) Primer3 on the WWW for general users and for biologist programmers. In: Krawetz S, Misener S (eds) Bioinformatics methods and protocols in the series methods in molecular biology. Humana Press, Totowa, pp 365–386. Source code available at http://primer3.sourceforge.net/

  • Ryder EJ (1967) A recessive male sterility gene in lettuce (Lactuca sativa L.). Proc Am Soc Hortic Sci 91:366–368

    Google Scholar 

  • Sasazaki S, Itoh K, Arimitsu S, Imada T, Takasuga A, Nagaishi H, Takano S, Mannen H, Tsuji S (2004) Development of breed identification markers derived from AFLP in beef cattle. Meat Sci 67:275–280

    Article  CAS  Google Scholar 

  • Tanksley SD, Young ND, Paterson AH, Bonierbale MW (1989) RFLP mapping in plant breeding: new tools for an old science. Biotechnology 7:257–264

    Article  CAS  Google Scholar 

  • Thomas CM, Vos P, Zebeau M, Jones DA, Norcott KA, Chardwick BP, Jones JDG (1995) Identification of amplified restriction fragment polymorphism (AFLP) markers tightly linked to the tomato Cf-9 gene for resistance to Cladosporium fulvum. Plant J 8:785–794

    Article  PubMed  CAS  Google Scholar 

  • Tsuchiya N (2009) Chouya No. 37, a fusarium root rot (race2)-resistant lettuce. J Jpn Soc Hortic Sci 78:206–210

    Article  Google Scholar 

  • Tsuchiya N, Yoshida K, Usui T, Tsukada M (2004) ‘Shinano hope’, a fusarium root rot-resistant lettuce. J Jpn Soc Hortic Sci 73:429–434

    Article  Google Scholar 

  • Van Ooijen JW, Voorrips RE (2001) JoinMap 3.0, software for the calculation of genetic linkage maps. Plant Research International, Wageningen

    Google Scholar 

  • Vos P, Hogers R, Bleeker M, Reijans M, van de Lee T, Hornes M, Freijters A, Pot J, Peleman J, Kuiper M, Zabeau M (1995) AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res 23:4407–4414

    Article  PubMed  CAS  Google Scholar 

  • Whitaker TW (1969) Salads for everyone—a look at the lettuce plant. Econ Bot 23:261–264

    Article  Google Scholar 

  • Zhang Y, Stommel JR (2001) Development of SCAR and CAPS markers linked to the Beta gene in tomato. Crop Sci 41:1602–1608

    Article  CAS  Google Scholar 

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Acknowledgments

The work was partly supported by a grant, Research Project for Utilizing Advanced Technologies in Agriculture, Forestry and Fisheries, from the Ministry of Agriculture, Forestry and Fisheries, Japan.

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Authors and Affiliations

  1. Laboratory of Genetics and Plant Breeding, Graduate School of Horticulture, Chiba University, 648 Matsudo, Matsudo City, Chiba, 271-8510, Japan

    M. Hayashi, A. Ujiie, H. Sassa, H. Kakui, T. Oda & T. Koba

  2. Nagano Vegetable and Ornamental Crops Experimental Station, 1066-1 Aza Tokoo, Oaza Souga, Siojiri City, Nagano, 399-6461, Japan

    H. Serizawa

Authors
  1. M. Hayashi
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  2. A. Ujiie
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  3. H. Serizawa
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  4. H. Sassa
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  5. H. Kakui
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  6. T. Oda
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  7. T. Koba
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Correspondence to T. Koba.

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Hayashi, M., Ujiie, A., Serizawa, H. et al. Development of SCAR and CAPS markers linked to a recessive male sterility gene in lettuce (Lactuca sativa L.). Euphytica 180, 429–436 (2011). https://doi.org/10.1007/s10681-011-0417-y

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  • DOI: https://doi.org/10.1007/s10681-011-0417-y

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