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In vivo efficacy of low-level laser therapy on bone regeneration

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Abstract

Purpose

In clinical use of low-level laser therapy for bone regeneration (LLLT), application protocol (dose, duration, and repetitions) has not been established. This study aimed to depict a reliable dosage of LLLT by evaluating the efficacy of different dosing of LLLT (diode) on the healing of rabbit cranial defects.

Methods

Critical size defects were prepared in calvarias of 26 New Zealand White Rabbits in such each animal containing both test and control groups. Test groups were irradiated with 4 Joule/cm2 (j/cm2), 6 j/cm2, and 8 j/cm2. The rabbits were subjected to six times of laser treatments in 10 days. At the end of the second week, 5 rabbits were sacrificed for histopathological and immunohistochemical analyses. At the 4th and 8th weeks, 20 rabbits (10 each) were sacrificed for micro-CT and histopathological analyses.

Results

Micro-CT evaluation revealed improved new bone formation in all test groups compared to the control group. 6 j/cm2 group demonstrated the highest bone formation. The highest bone morphogenic protein -2 levels were found in the 4 j/cm2 group. Osteocalcin expression was significantly higher in 4 j/cm2 group.

Conclusions

Our findings indicate that LLLT have a positive effect on new bone formation. The high efficacy of doses of 4 j/cm2 and 6 j/cm2 is promising to promote early bone healing.

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Acknowledgements

The authors express their gratitude to Hacettepe University Advanced Technologies Application and Research Center and also like to thank Ayhan Parmaksiz due to his significant contribution to statistical analyses of the study.

Funding

This project had been supported by Hacettepe University Scientific Research Projects Coordination Unit (Grant no: TSA-2017–14499).

Hacettepe Üniversitesi,Grant no: TSA-2017–14499,Birtan Tolga Yılmaz

Author information

Authors and Affiliations

  1. Periodontology Department, Faculty of Dentistry, Hacettepe University, Ankara, Turkey

    Birtan Tolga Yılmaz, Abdullah C. Akman, Güliz N. Güncü & Rahime M. Nohutcu

  2. Surgical Research Lab, Faculty of Medicine, Hacettepe University, Ankara, Turkey

    Alper Çetinkaya

  3. Computer Engineering, Middle East Technical University, Ankara, Turkey

    Can Colak

  4. Oral Pathology Department, Faculty of Dentistry, Gazi University, Ankara, Turkey

    Benay Yıldırım

  5. Department of Periodontology, Cyprus Health and Social Sciences University, Ankara, Turkey

    Özlem Özer Yücel

Authors
  1. Birtan Tolga Yılmaz
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  2. Abdullah C. Akman
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  3. Alper Çetinkaya
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  4. Can Colak
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  5. Benay Yıldırım
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  6. Özlem Özer Yücel
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  7. Güliz N. Güncü
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  8. Rahime M. Nohutcu
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Correspondence to Birtan Tolga Yılmaz.

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The authors declare no competing interests.

Ethics approval

The study protocol was approved by Hacettepe University Animal Experiments Local Ethics Committee (date/number:28.03.2017/52338575).

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Yılmaz, B.T., Akman, A.C., Çetinkaya, A. et al. In vivo efficacy of low-level laser therapy on bone regeneration. Lasers Med Sci 37, 2209–2216 (2022). https://doi.org/10.1007/s10103-021-03487-8

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  • DOI: https://doi.org/10.1007/s10103-021-03487-8

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