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Low-level laser therapy induces the expressions of BMP-2, osteocalcin, and TGF-β1 in hypoxic-cultured human osteoblasts

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Abstract

The aim of this study was to examine the effect of low-level laser therapy (LLLT) on the cell viability and the expression of hypoxia-inducible factor-1s (HIF-1s), bone morphogenic protein-2 (BMP-2), osteocalcin, type I collagen, transforming growth factor-β1 (TGF-β1), and Akt in hypoxic-cultured human osteoblasts. Human fetal osteoblast cells (cell line 1.19) were cultured under 1 % oxygen tension for 72 h. Cell cultures were divided into two groups. At the experimental side, low-level laser (808 nm, GaAlAs diode) was applied at 0, 24, and 48 h. After irradiation, each cell culture was incubated 24 h more under hypoxia. Total energy was 1.2, 2.4, and 3.6 J/cm2, respectively. Non-irradiated cultures served as controls. Comparisons between the two groups were analyzed by t test; a p value <0.05 was considered statistically significant. Hypoxia resulted in a decrease in the expression of type I collagen, osteocalcin, and TGF-β1 (p < 0.001, p < 0.001, and p < 0.01, respectively). Cell viability and BMP-2 expression were not decreased by hypoxic condition. On the other hand, LLLT on hypoxic-cultured osteoblast promoted the expression of BMP-2, osteocalcin, and TGF-β1 (p < 0.05, p < 0.01, and p < 0.001, respectively). Cell proliferation was also increased time-dependently. However, hypoxia decreased in type I collagen expression (p < 0.001), and LLLT did not affect type I collagen expression in hypoxic-cultured osteoblasts. Furthermore, LLLT inhibited HIF-1 and Akt expression in hypoxic conditioned osteoblasts. We concluded that LLLT induces the expression of BMP-2, osteocalcin, and TGF- β1 in 1 % hypoxic-cultured human osteoblasts.

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Acknowledgments

This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2011–0026921).

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Authors and Affiliations

  1. Department of Oral and Maxillofacial Surgery, School of Dentistry, Pusan National University, Beomeo, Mulgum, Yangsan, 626-770, Republic of Korea

    Se-Jeong Pyo, Won-Wook Song, Sang-Hun Shin, In-Kyo Chung & Yong-Deok Kim

  2. Department of Oral Anatomy, School of Dentistry, Pusan National University, Yangsan, Republic of Korea

    In-Ryoung Kim & Bong-Soo Park

  3. Department of Dental Anesthesia and Pain Medicine, School of Dentistry, Pusan National University, Yangsan, Republic of Korea

    Cheul-Hong Kim

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  1. Se-Jeong Pyo
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Correspondence to Yong-Deok Kim.

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Dr. Song WW and Dr. Pyo SJ equally contributed to this study as the first authors.

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Pyo, SJ., Song, WW., Kim, IR. et al. Low-level laser therapy induces the expressions of BMP-2, osteocalcin, and TGF-β1 in hypoxic-cultured human osteoblasts. Lasers Med Sci 28, 543–550 (2013). https://doi.org/10.1007/s10103-012-1109-0

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