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Effects of low-level laser therapy on bone formed after distraction osteogenesis

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Abstract

This study evaluated the effect of low-level laser therapy (LLLT) on the chemical composition, crystallinity and crystalline structure of bone at the site of distraction osteogenesis. Five rabbits were subjected to distraction osteogenesis (latency = 3 days; rate and frequency = 0.7 mm/day for 7 days; consolidation = 10 days), and three were given LLLT with arsenide–gallium–aluminum (AsGaAl; 830 nm, 40 mW): 10 J/cm2 dose per spot, applied directly to the distraction osteogenesis site during the consolidation stage at 48 h intervals. Samples were harvested at the end of the consolidation stage. X-ray fluorescence and X-ray diffraction were used to analyze chemical composition, crystallinity and crystalline structure of bone at the distraction osteogenesis site. The analysis of chemical composition and calcium (Ca) and phosphorus (P) ratios revealed greater mineralization in the LLLT group. Diffractograms showed that the crystalline structure of the samples was similar to that of hydroxyapatites. Crystallinity percentages were greater in rabbits that were given LLLT. Crystallinity (41.14% to 54.57%) and the chemical composition of the bone at the distraction osteogenesis site were similar to the that of the control group (42.37% to 49.29%). The results showed that LLLT had a positive effect on the biomodulation of newly formed bone.

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Acknowledgments

We thank the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Brazil, for financial support for this study. We also thank Dr. Adriana Etges from Universidade Federal de Pelotas, Brazil.

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Authors and Affiliations

  1. School of Physics, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Av. Ipiranga, 6681, Prédio 10, sala 222, Caixa Postal 1429, 90619-900, Porto Alegre, RS, Brazil

    Roberto Hübler & Eduardo Blando

  2. School of Dentistry, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Porto Alegre, RS, Brazil

    Lêonilson Gaião, Paulo Eduardo Kreisner & Marília Gerhardt de Oliveira

  3. School of Dentistry, Universidade Federal de Pelotas (UFPel), Pelotas, RS, Brazil

    Letícia Kirst Post & Cristina Braga Xavier

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  1. Roberto Hübler
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  2. Eduardo Blando
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  3. Lêonilson Gaião
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  4. Paulo Eduardo Kreisner
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  5. Letícia Kirst Post
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  7. Marília Gerhardt de Oliveira
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Correspondence to Marília Gerhardt de Oliveira.

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Hübler, R., Blando, E., Gaião, L. et al. Effects of low-level laser therapy on bone formed after distraction osteogenesis. Lasers Med Sci 25, 213–219 (2010). https://doi.org/10.1007/s10103-009-0691-2

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  • DOI: https://doi.org/10.1007/s10103-009-0691-2

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