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Aptamer based surface plasmon resonance sensor for aflatoxin B1

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Abstract

The authors describe a surface plasmon resonance (SPR) based aptasensor for the carcinogenic mycotoxin aflatoxin B1 (AFB1) in a direct assay format. The aptamer is immobilized on the surface of a commercial sensor chip, and the SPR signal increases on binding of AFB1. The sensor chip can be fully regenerated by passing a flow of buffer over it upon which bound AFB1 dissociates from the aptamer. The biosensor works in the 0.4 nM to 200 nM AFB1 concentration range and has a 0.4 nM detection limit. It allows AFB1 to be determined in complex samples such as diluted red wine and beer. The assay is sensitive, and the chip is easily regenerated and stable. The method therefore overcomes certain limitations of antibody-based SPR assays and of competitive SPR assays for AFB1.

Schematic presentation of the assay: Aptamer is coated on the chip of SPR, and the binding between aflatoxin B1 (AFB1) and the aptamer on chip causes SPR responses, allowing sensitive detection of AFB1.

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Acknowledgements

This work was supported by National Natural Science Foundation of China (Grant No. 21575153, 21435008), Strategic Priority Research Program of the Chinese Academy of Sciences (XDB14030200), and the Key Research Program of the Chinese Academy of Sciences (KFZD-SW-203). We would be grateful to Yuanyuan Chen, Zhenwei Yang (Institute of Biophysics, Chinese Academy of Sciences) for technical help with SPR experiments on Biacore instrument.

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Authors and Affiliations

  1. State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China

    Linlin Sun & Qiang Zhao

  2. University of Chinese Academy of Sciences, Beijing, 100049, China

    Linlin Sun & Qiang Zhao

  3. National Institute of Metrology, Beijing, 100029, China

    Liqing Wu

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  1. Linlin Sun
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  2. Liqing Wu
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Correspondence to Qiang Zhao.

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Sun, L., Wu, L. & Zhao, Q. Aptamer based surface plasmon resonance sensor for aflatoxin B1. Microchim Acta 184, 2605–2610 (2017). https://doi.org/10.1007/s00604-017-2265-5

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