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Recent trends in SELEX technique and its application to food safety monitoring

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Abstract

The method referred to as “systemic evolution of ligands by exponential enrichment” (SELEX) was introduced in 1990 and ever since has become an important tool for the identification and screening of aptamers. Such nucleic acids can recognize and bind to their corresponding targets (analytes) with high selectivity and affinity, and aptamers therefore have become attractive alternatives to traditional antibodies not the least because they are much more stable. Meanwhile, they have found numerous applications in different fields including food quality and safety monitoring. This review first gives an introduction into the selection process and to the evolution of SELEX, then covers applications of aptamers in the surveillance of food safety (with subsections on absorptiometric, electrochemical, fluorescent and other methods), and then gives conclusions and perspectives. The SELEX method excels by its features of in vitro, high throughput and ease of operation. This review contains 86 references.

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Aptamers, novel recognition probes screened with SELEX, have been adopted as substitution to antibody in various fields and also widely applied in food safety monitoring.

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Acknowledgments

This work is financially supported by the Huangshan Young Scholar Fund of Hefei University of Technology (407-037025), the National Natural Science Foundation of China with grant 31328009 and the NSF of Jiangsu Province (BK20130379, 13KJB550001), the Science and Technology Research Project of General Administration of Quality Supervision, Inspection and Quarantine of P. R. China (201210127, 201310135), the 12th Five Years Key Programs (2012BAK08B01-2, 2012BAK17B10, SS2012AA101001), Suzhou Science and Technology Committee Program (SS201335) and the Fundamental Research Funds for the Central Universities (2013HGCH0008, 2012HGCX0003).

G Liu acknowledges financial support from the National Cancer Institute (Grant number: R21CA137703) and the National Institute of General Medicine (NIGMS) (5P30 GM103332). Its contents are solely the responsibility of the authors and do not necessarily represent the official views of the NIH.

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Authors and Affiliations

  1. School of Biotechnology and Food Engineering, Anhui Provincial Key Lab of Functional Materials & Devices, Hefei University of Technology, Hefei, 230009, People’s Republic of China

    Jingjing Wu, Feng Xue, Zhanlong Mei, Li Yao, Xin Wang, Jian Liu & Wei Chen

  2. School of Biotechnology and Food Engineering, Changshu Institute of Technology, Changshu, 215500, People’s Republic of China

    Yingyue Zhu

  3. School of Medical Engineering, Hefei University of Technology, Hefei, 230009, People’s Republic of China

    Lei Zheng

  4. Department of Chemistry and Biochemistry, North Dakota State University, Fargo, ND, 58108, USA

    Guodong Liu

  5. School Food Science & Technology, Jiangnan University, Wuxi, 214122, People’s Republic of China

    Chifang Peng

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  1. Jingjing Wu
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Correspondence to Guodong Liu or Wei Chen.

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Jingjing Wu, Yingyue Zhu, and Feng Xue contributed equally to this paper.

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Wu, J., Zhu, Y., Xue, F. et al. Recent trends in SELEX technique and its application to food safety monitoring. Microchim Acta 181, 479–491 (2014). https://doi.org/10.1007/s00604-013-1156-7

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