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Molecular alterations of bone quality in sequesters of bisphosphonates-related osteonecrosis of the jaws

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Abstract

Summary

Compared to healthy bone, the intrinsic bone materials properties in the pre-existing lamellar bone are altered in jaw bone sequesters of bisphosphonates (BP)-related osteonecrosis.

Introduction

The aim of this study was to evaluate the human jaw bone quality, especially intrinsic bone material properties among sequesters of osteonecrosis of the jaw (ONJ) induced by BP.

Methods

Bone sequesters were obtained from 24 patients suffering from ONJ following a BP treatment. Within BP-exposed bone samples, benign-BP and malignant-BP groups were distinguished in relation to the underlying disease: osteoporosis and bone metastases or multiple myeloma, respectively. Healthy cadaveric cortical jaw bone samples were used as controls. The physicochemical parameters of bone samples — mineral/organic ratio, relative proteoglycan content, crystallinity, monohydrogen phosphate content, and type-B carbonate substitution — were evaluated by Raman microspectroscopy. Representative Raman spectral features of bones control and BP-exposed bone sequesters were identified with the Partial-Least-Square Discriminant Analysis (PLS-DA).

Results

BP-exposed bone sequesters are characterized by a significant increase of mineral to organic ratio (+12 %) and a significant decrease of relative proteoglycan content (−35 %), thus regulating initial collagen matrix mineral deposition. Structural changes on mineral components are revealed by a significant decrease of both crystallinity (−2 %) and mineral maturation (−41 %) in the BP-exposed bone sequesters compared to healthy bones. These modifications were also observed distinctly in both benign-BP and malignant-BP groups. In addition, a shift of the phosphate ν1 band was highlighted by PLS-DA between bones control and BP-exposed bone sequesters, revealing a disruption of the apatitic phosphate environment in the jaw bone sequesters.

Conclusions

The present data show that jaw bone quality can be altered with an overmineralization and ultrastructural modifications of apatitic mineral in bone sequesters of BP-related ONJ.

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Abbreviations

ALN:

Alendronate

BP:

Bisphosphonate

CLO:

Clodronate

CTL:

Control

GAG:

Glycosaminoglycan

IBN:

Ibandronate

ONJ:

Osteonecrosis of the jaw

PAM:

Pamidronate

PG:

Proteoglycan

PLS-DA:

Partial-Least-Square Discriminant Analysis

ZOL:

Zoledronate

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Acknowledgments

The authors thank the Anatomy Laboratory of Medical School, Lille, France, for supplying bone control samples. We also thank O. Devos of Laboratoire de Spectrochimie Infrarouge et Raman (LASIR), UMR 8516 for his help on the PLS-DA analyses. We thanks the Institut Français pour la Recherche Odontologique (IFRO) and the Société Française de Chirurgie Orale (SFCO) for their financial support.

Conflicts of interest

BC: occasional interventions: consultancy or speaker fees from Amgen, Daiichi-Sankyo, Ferring, GSK, Lilly, MSD, Medtronic, Novartis, and Servier. Indirect interests: financial support for research programs or investigator fees from Amgen, Lilly, MSD, Novartis, and Roche. MHV: occasional interventions: consultancy or speaker fees from Amgen, Medtronic and Novartis.

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Authors and Affiliations

  1. Université Lille Nord de France, 59000, Lille, France

    C. Olejnik, G. Falgayrac, A. During, M. H. Vieillard, B. Cortet & G. Penel

  2. EA 4490 PMOI, Faculté de Chirurgie Dentaire, Place de Verdun, IFR 114 IMPRT, 59000, Lille, France

    C. Olejnik, G. Falgayrac, A. During, M. H. Vieillard, B. Cortet & G. Penel

  3. Service de Rhumatologie, Hôpital Roger Salengro, CHRU de Lille, 59000, Lille, France

    M. H. Vieillard & B. Cortet

  4. Service d’Oncologie Générale, Centre Oscar Lambret, Lille, 59020, France

    M. H. Vieillard

  5. Service de Chirurgie Maxillo-Faciale et Stomatologie, Hôpital Roger Salengro, CHRU de Lille, 59000, Lille, France

    J. M. Maes

  6. Service d’Odontologie, Centre Abel Caumartin, CHRU de Lille, 59000, Lille, France

    G. Penel

  7. Faculté de Chirurgie Dentaire, Place de Verdun, 59000, Lille, France

    G. Penel

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  1. C. Olejnik
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  2. G. Falgayrac
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  3. A. During
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  4. M. H. Vieillard
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  7. G. Penel
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Correspondence to C. Olejnik.

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Olejnik, C., Falgayrac, G., During, A. et al. Molecular alterations of bone quality in sequesters of bisphosphonates-related osteonecrosis of the jaws. Osteoporos Int 25, 747–756 (2014). https://doi.org/10.1007/s00198-013-2514-3

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  • DOI: https://doi.org/10.1007/s00198-013-2514-3

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