Abstract
Ammonia exchange with the atmosphere was studied in barley (Hordeum vulgare L. cv. Golf) grown in nutrient solution. Ammonia emission from the leaves was evident when NH4 + was taken up by the roots or when the plants had been subjected to darkness for 3 to 7 days. Also NH4 + concentrations in shoot and root tissues increased with these treatments while the activity of the ammonium assimilating enzyme glutamine synthetase (GS) increased in the roots with increasing NH4 + concentrations supplied to the medium and decreased in both shoot and root after 3 days of dark-induced senescence.
Barley mutant plants (cv. Maris Mink) with only 66 or 47% of normal GS activity showed higher tissue NH4 + concentrations, higher NH3 emission and a greater sensitivity to increased temperature than wild type barley plants. The 66% GS mutant always showed higher NH3 emission compared to plants with the lowest GS activity (47%), probably due to a mechanism preventing tissue NH4 + concentrations from increasing too much. Apoplastic NH4 + and pH also increased in the GS mutants and estimated compensation points for NH3 were higher compared to wild type plants.
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© 1997 Kluwer Academic Publishers
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Mattsson, M., Schjoerring, J.K. (1997). Ammonia exchange between plants and the atmosphere: Effects of ammonium supply to the roots, dark-induced senescence and reduced GS activity. In: Ando, T., Fujita, K., Mae, T., Matsumoto, H., Mori, S., Sekiya, J. (eds) Plant Nutrition for Sustainable Food Production and Environment. Developments in Plant and Soil Sciences, vol 78. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-0047-9_268
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DOI: https://doi.org/10.1007/978-94-009-0047-9_268
Publisher Name: Springer, Dordrecht
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