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Scale-up of 5-keto-Gluconic Acid Production by Gluconobacter oxydans HGI-1

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Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012)

Part of the book series: Lecture Notes in Electrical Engineering ((LNEE,volume 249))

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Abstract

Gluconobacter oxydans is known to oxidize glucose to gluconic acid (GA), and subsequently, to 2-keto-gluconic acid (2KGA) and 5-keto-gluconic acid (5KGA). 5KGA can be converted to L-(+)-tartaric acid which is important in industry. In order to increase the production of 5KGA, G. oxydans HGI-1 which converts GA only to 5KGA was chosen in this study. The fermentation process occurred in shake flask was studied in different inoculation, and 10 % inoculation amount was chosen. Furthermore, it was scaled-up to a 5-L stirred-tank fermenter, limitation of oxygen could not happen and pH could be adjusted by automated titration of 6 M KOH. 5KGA was accumulated up to 106.33 g/L in fermentation broth, while the strain was fed-batch cultured, the concentration of 5KGA was up to 179.4 g/L and the conversion rate of glucose was 89.0 %. G. oxydans HGI-1 was suitable for industrial production.

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Authors and Affiliations

  1. Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, College of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, People’s Republic of China

    Yanyan Li, Shiru Jia, Cheng Zhong, Hongcui Wang, Ainan Guo & Xintong Zheng

Authors
  1. Yanyan Li
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  2. Shiru Jia
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  3. Cheng Zhong
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  4. Hongcui Wang
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  5. Ainan Guo
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  6. Xintong Zheng
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Corresponding author

Correspondence to Shiru Jia .

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Editors and Affiliations

  1. College of Bioengineering, Tianjin University of Science and Technology, Tianjin, People's Republic of China

    Tong-Cun Zhang

  2. Nanjing University of Technology, Nanjing, People's Republic of China

    Pingkai Ouyang

  3. Department of Microbiology & Molecular Genetics, University of Texas Houston Medical School, Texas, Texas, USA

    Samuel Kaplan

  4. Wellcome Trust Sanger Institute, Cambridge, United Kingdom

    Bill Skarnes

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Li, Y., Jia, S., Zhong, C., Wang, H., Guo, A., Zheng, X. (2014). Scale-up of 5-keto-Gluconic Acid Production by Gluconobacter oxydans HGI-1. In: Zhang, TC., Ouyang, P., Kaplan, S., Skarnes, B. (eds) Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012). Lecture Notes in Electrical Engineering, vol 249. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-37916-1_31

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  • DOI: https://doi.org/10.1007/978-3-642-37916-1_31

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  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-37915-4

  • Online ISBN: 978-3-642-37916-1

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