Abstract
Gluconobacter oxydans converts glucose to gluconic acid and subsequently to 2-keto-d-gluconic acid (2-KGA) and 5-keto-d-gluconic acid (5-KGA) by membrane-bound periplasmic pyrroloquinoline quinone-dependent and flavin-dependent dehydrogenases. The product pattern obtained with several strains differed significantly. To increase the production of 5-KGA, which can be converted to industrially important l-(+)-tartaric acid, growth parameters were optimized. Whereas resting cells of G. oxydans ATCC 621H converted about 11% of the available glucose to 2-KGA and 6% to 5-KGA, with growing cells and improved growth under defined conditions (pH 5, 10% pO2, 0.05% pCO2) a conversion yield of about 45% 5-KGA from the available glucose was achieved. As the accumulation of the by-product 2-KGA is highly disadvantageous for an industrial application of G. oxydans, a mutant was generated in which the membrane-bound gluconate-2-dehydrogenase complex was inactivated. This mutant, MF1, grew in a similar way to the wild type, but formation of the undesired 2-KGA was not observed. Under improved growth conditions, mutant MF1 converted the available glucose almost completely (84%) into 5-KGA. Therefore, this newly developed recombinant strain is suitable for the industrial production of 5-KGA.
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The project was carried out within the framework of the Competence Network Göttingen “Genome research on bacteria” (GenoMik) financed by the German Federal Ministry of Education and Research (BMBF).
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Elfari, M., Ha, SW., Bremus, C. et al. A Gluconobacter oxydans mutant converting glucose almost quantitatively to 5-keto-d-gluconic acid. Appl Microbiol Biotechnol 66, 668–674 (2005). https://doi.org/10.1007/s00253-004-1721-4
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DOI: https://doi.org/10.1007/s00253-004-1721-4