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The Role of Fungal Transcriptome Analysis and Side-Chain Hydrolyzing Enzymes in Sugarcane Bagasse Breakdown

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Advances of Basic Science for Second Generation Bioethanol from Sugarcane

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Abstract

Lignocellulose represents one of the main sources of renewable energy for biofuel production. Within this context, sugarcane bagasse, which is a discarded by-product of sugarcane processing, is a rich source of lignin, hemicellulose, and cellulose. Since sugarcane bagasse is a complex lignocellulosic structure, its complete enzymatic hydrolysis requires the action of an enzyme system of main- and side-chain enzymes. The xylan component in sugarcane bagasse is arabinosylated at positions C-2 and/or C-3 and esterified with ferulic acid at the C-5 position. Accessory enzymes such as α-arabinofuranosidase and feruloyl esterase are responsible for enhanced accessibility and enzymatic hydrolysis of xylan. Under industrial conditions, numerous filamentous fungi can secrete enzymes or enzyme complexes belonging to the glycosyl hydrolases, carbohydrate esterases, and polysaccharide lyases for the hydrolysis of cellulose, hemicellulose, and lignin. Next-generation sequencing approaches for whole-genome and transcriptome analyses enable the identification of novel genes and isoforms in fungi encoding hydrolytic enzymes, thus increasing our understanding of mechanisms involved in gene expression induction in the presence of plant cell wall or its metabolic products. The downstream application will accelerate strain improvement for increased enzymatic efficiencies and hydrolytic enzyme cocktail development, contributing to reducing cellulosic bioethanol production costs.

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Acknowledgements

The authors acknowledge the financial support from the Brazilian Research Council (CNPq), Coordination for the Improvement of Higher Level Personnel (CAPES), the Foundation for Research Support of Federal District (FAPDF), and the National Institute of Science and Technology of Bioethanol.

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    1. Department of Cellular Biology, University of Brasília, Brasília, DF, CEP 70910-900, Brazil

      Leonora Rios de Souza Moreira, Camila Louly Corrêa, Helder Andrey Rocha Gomes, Glaucia Emy Okida Midorikawa, Robert Neil Gerard Miller & Edivaldo Ximenes Ferreira Filho

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    1. Leonora Rios de Souza Moreira
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    2. Camila Louly Corrêa
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    Correspondence to Edivaldo Ximenes Ferreira Filho .

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    1. Department of Botany, Laboratory of Plant Physiological Ecology (LAFIECO), Institute of Biosciences, University of São Paulo, São Paulo, Brazil

      Marcos S. Buckeridge

    2. Department of Botany, Laboratory of Plant Physiological Ecology (LAFIECO), Institute of Biosciences, University of São Paulo, São Paulo, Brazil

      Amanda P. De Souza

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    de Souza Moreira, L.R., Corrêa, C.L., Gomes, H.A.R., Midorikawa, G.E.O., Miller, R.N.G., Filho, E.X.F. (2017). The Role of Fungal Transcriptome Analysis and Side-Chain Hydrolyzing Enzymes in Sugarcane Bagasse Breakdown. In: Buckeridge, M., De Souza, A. (eds) Advances of Basic Science for Second Generation Bioethanol from Sugarcane. Springer, Cham. https://doi.org/10.1007/978-3-319-49826-3_6

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