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Transcriptional analysis of genes for energy catabolism and hydrolytic enzymes in the filamentous fungus Aspergillus oryzae using cDNA microarrays and expressed sequence tags

  • Genomics and Proteomics
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Abstract

Aspergillus oryzae is a fungus used extensively in the fermentation industry. We constructed cDNA microarrays comprising 2,070 highly expressed cDNAs selected from the ∼6,000 non-redundant expressed sequence tags (ESTs) in the A. oryzae EST database (http://www.aist.go.jp/RIODB/ffdb/index.html). Using the cDNA microarrays, we analyzed the gene expression profiles of A. oryzae cells grown under the glucose-rich (AC) and glucose-depleted (AN) liquid culture conditions used during the construction of the EST database. The sets of genes identified by the cDNA microarray as highly expressed under each culture condition agreed well with the highly redundant ESTs obtained under the same conditions. In particular, transcription levels of most catabolic genes of the glycolytic pathway (EMP) and tricarboxylic acid (TCA) cycle were higher under AC than AN conditions, suggesting that A. oryzae uses both EMP and TCA for glucose metabolism under AC conditions. We further studied the expression of genes encoding hydrolytic enzymes and enzymes involved in energy catabolism by using three industrial solid-phase biomass media, including wheat-bran. The wheat-bran culture gave the richest gene expression profile of hydrolytic enzymes and the lowest expression levels of catabolic genes (EMP, TCA) among the three media tested. The low expression levels of catabolic genes in the wheat-bran culture may release catabolite repression, consequently leading to the rich expression profiles of the hydrolytic enzymes.

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Acknowledgements

This work was supported in part by a grant-in-aid (Proposal-based Energy/Environment International Joint Research Program) from the New Energy and Industrial Technology Development Organization (NEDO) of Japan as well as a grant-in-aid (Bio Design Program) from the Ministry of Agriculture, Forestry and Fisheries of Japan (BDP-03-VI-1-6). Hiroshi Maeda is supported in part by a grant-in-aid from Noda Institute for Scientific Research. We thank for Jiujiang Yu and Gary Payne for providing several cDNA clones. This manuscript is in memory of Makoto Watanabe.

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Authors and Affiliations

  1. Department of Molecular and Cell Biology, Graduate School of Agricultural Science, Tohoku University, 1-1 Amamiya, Tsutsumi-dori, Sendai, 981-8555, Japan

    Hiroshi Maeda, Yutaka Maruyama, Takeki Tanno, Yoshiteru Totsuka, Youhei Yamagata, Keietsu Abe, Katsuya Gomi & Tasuku Nakajima

  2. The New Industry Creation Hatchery Center, Tohoku University, Sendai, 980-8579, Japan

    Hiroshi Maeda, Yutaka Maruyama, Youhei Yamagata, Fumihiko Hasegawa, Keietsu Abe, Katsuya Gomi, Tasuku Nakajima & Yasutaka Iguchi

  3. Tohoku Technoarch Co. Ltd, Sendai, 981-0845, Japan

    Hiroshi Maeda

  4. Research Center for Glycoscience, National Institute of Advanced Industrial Science and Technology, Tsukuba, 305-8566, Japan

    Motoaki Sano & Masayuki Machida

  5. National Research Institute of Brewing, Higashi-Hiroshima, 739-0046, Japan

    Takeshi Akao & Osamu Akita

  6. Industrial Technology Institute, Miyagi Prefectural Government, Sendai, 981-3206, Japan

    Misako Endo & Rumi Sakurada

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  1. Hiroshi Maeda
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  2. Motoaki Sano
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  3. Yutaka Maruyama
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  9. Youhei Yamagata
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  10. Masayuki Machida
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  11. Osamu Akita
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  12. Fumihiko Hasegawa
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  13. Keietsu Abe
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  15. Tasuku Nakajima
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  16. Yasutaka Iguchi
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Correspondence to Keietsu Abe.

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Maeda, H., Sano, M., Maruyama, Y. et al. Transcriptional analysis of genes for energy catabolism and hydrolytic enzymes in the filamentous fungus Aspergillus oryzae using cDNA microarrays and expressed sequence tags. Appl Microbiol Biotechnol 65, 74–83 (2004). https://doi.org/10.1007/s00253-004-1608-4

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  • DOI: https://doi.org/10.1007/s00253-004-1608-4

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