Abstract
The introduction, establishment, and expression of foreign genes in the nuclei of plant cells involves three basic steps: the introduction of DNA into the cell, the identification and propagation of the transformed cell, and the evaluation of expression levels of the gene or genes of interest. This chapter examines the molecular tools required for the successful completion of plant transformation and desired gene expression. The goal is to illustrate some of the ideas and selection criteria that enter into the decision of which of the many choices available may best fit the object of the exercise. Particular components are mentioned to illustrate a specific point, because, as the field advances, the number of options steadily increases. A wide range of published information is available for more in-depth pursuit of a specific topic.
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Merlo, D.J. (2003). Molecular Tools for Engineering Plant Cells. In: Vinci, V.A., Parekh, S.R. (eds) Handbook of Industrial Cell Culture. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59259-346-0_9
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